Crystal structure determination of KREMEN1, DICKKOPF1 and MeCP2
Crystal structure determination of KREMEN1, DICKKOPF1 and MeCP2
... CRYSTAL STRUCTURE DETERMINATION OF KREMEN1, DICKKOPF1 AND MeCP2 VINDHYA B.N.REDDY A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF BIOLOGICAL SCIENCES ... INTERACTION OF DKK-1/KRM/LRP5/6 DNA METHYLATION 11 1.5.1 Methyl-CpG binding proteins (MeCP2) 12 1.5.2 Structure of MBDs 13 1.5.3 MeCP2 15 1.5.4 Architecture of MeCP2 16 1.5.5 Role of...
Báo cáo khoa học: ˚ cDNA cloning and 1.75 A crystal structure determination of PPL2, an endochitinase and N-acetylglucosaminebinding hemagglutinin from Parkia platycephala seeds potx
... Cavada et al PPL2, an endochitinase from Parkia platycephala Fig Crystal structure of PPL2 (A) and (B) show two views of the (ab)8 barrel fold of PPL2 The a- helices (red) and b-strands (yellow) are ... The Authors Journal compilation ª 2006 FEBS 3963 PPL2, an endochitinase from Parkia platycephala B S Cavada et al Fig Purification and molecular mass d...
Crystal structure determination of HEX1 and human GEMININ 70 152
... Founded 1905 CRYSTAL STRUCTURE DETERMINATION OF HEX1 AND HUMAN GEMININ7 0 -152 YUAN PING (B.S., M.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY INSTITUTE OF MOLECULAR AND CELL BIOLOGY ... Function test of Geminin7 0 -152 ···················································97 3.2.3 Expression and purification of Geminin7 0 -152 ··················...
Báo cáo khoa học: Crystal structure determination and inhibition studies of a novel xylanase and a-amylase inhibitor protein (XAIP) from Scadoxus multiflorus pot
... kDa, a second peak of 30 kDa and a third peak of about 20 kDa A similar gel filtration experiment was also carried out for the complex of XAIP with a- amylase A mixture of XAIP and a- amylase was ... observations indicate that XAIP associates with GH11 xylanase and GH13 a- amylase, as well as with both xylanase and a- amylase simultaneously Tissue distribution...
NMR study of the human NCK2 SH3 domains structure determination, binding diversity, folding and amyloidogenesis 1
... properties of the hNck2 SH3 domains 1. 5.2 Secondary structure transition study of hNck2 SH3- 1 domain The unfolding of SH3- 1 induced by the pH change and transition from the β-sheet dominant to the helical ... main binding region of DOCK180 was located at region 18 19 -18 36 In addition, two of the other weak binding sites were pinpointed at the...
NMR study of the human NCK2 SH3 domains structure determination, binding diversity, folding and amyloidogenesis 2
... relaxation delays of 10, 30, 45, 60, 75, 90 and 150ms 15 N T1 and T2 relaxation times and {1H}-15N steady-state NOEs of SH3- 1-V 22 52 (insertion of Val at 22 nd) were determined at 20 °C 15 N T1 values ... delays of 10, 500, 50, 300, 100, 400 and 20 0ms 15 N T2 values were determined with relaxation delays of 10, 50, 80, 120 , 150, 180, 21 0, 23 0 and 25 0 ms 15...
NMR study of the human NCK2 SH3 domains structure determination, binding diversity, folding and amyloidogenesis 3
... characteristic of all SH3 domains Figure 3. 3C shows a superimposition of hNck2 SH3- 2 and Nck1 SH3- 2 domains recently 60 Figure 3. 2 HSQC and sequential assignments of SH3- 2 and SH3- 3 A shows the HSQC and ... 0 .37 Secondary structure regions 64 3. 2.2 HSQC identification of the residues involved in binding of the hNck2 SH3 Domains The inte...
NMR study of the human NCK2 SH3 domains structure determination, binding diversity, folding and amyloidogenesis 4
... β-sheets The first, the last and the first half of the second β-strand constitute the first β-sheet, whereas the second half of the second β-strand, together with the third and fourth β-strands constitutes ... with the lowest target function and its ensemble of ten structures superimposed over residues 32 44 Right: Ribbon representation of the NMR structur...
NMR study of the human NCK2 SH3 domains structure determination, binding diversity, folding and amyloidogenesis 5
... HG Val53 HN Tyr52 HN Glu54 HN Arg 55 HN Glu54 HN Val53 HN Glu54 HN Tyr52 HB Arg 55 HN Val53 HG Arg 55 HN Val53 HB Table 5. 2 Native like long range NOEs Between the first and second beta strands Val3 ... between the SH3- 1-V22 and WT at and 1H frequencies, especially at the N- and C-terminals of SH3- 1 1 25 Figure 5. 3 Assignments of SH3- 1-V22 at pH 4.0 and V22△...
NMR study of the human NCK2 SH3 domains structure determination, binding diversity, folding and amyloidogenesis 6
... both the CD and NMR spectra; the NMR titration experiment shows that the binding event mainly happens in the Cterminal of the ApLLP; 2) Consistent with the NMR study, the CD spectra show that there ... 159 Figure 6. 5 Bindings of the CRE DNA to two ApLLP proteins A–B) Superimposition of the 1H–15N NMR HSQC spectra of ApLLP-87, A) and ApLLP-55, B) in...
NMR study of the human NCK2 SH3 domains structure determination, binding diversity, folding and amyloidogenesis 7
... 7. 2 Future work 1) Structure determinations and study of binding diversity are carried out for both SH3- 2 and SH3- 3 domain in this project However, whether the hydrogen bond ... between SH3 and proline-rich peptides The dynamic information of loops and 310-helix in the complex system will give us more functional implication 3) If the solubility and stabilit...
Magnetic resonance spectroscopy correlation with histological analysis in gliomas and structure determination of a hypothetical protein
... N-Acetyl aspartate (NAA), N-Acetylaspartylglutamate (NAAG), Alanine, Choline, Creatine, Glutamate, Glycine, Myo-inositol, Lactate, Pyruvate, Serine, Threonine, Tyrosine and Valine Acetate is an ... Magnetic resonance spectroscopy correlation with histological analysis in gliomas and structure determination of a hypothetical protein Xu Ying A THESIS SUBMITTE...
báo cáo hóa học:" Research Article Determination of Nonprototypical Valence and Arousal in Popular Music: Features and Performances" potx
... precision (pre), and mean recall (rec) for training with the training and development instances of AllInst, and testing on those of AllInst, Min2/4, and Min3/4 NoLyr feature set, V3 and A3 tasks, ... 3/4 (b) Arousal Figure 8: Distributions of test instances in dependence of prototypicality: AllInst, Min2/4 (minimum of raters agree), and Min3/4 (minimum of raters ag...
Báo cáo vật lý: "SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF Pb(II) AND Cd(II) USING ARTIFICIAL NEURAL NETWORKS" potx
... Pb(II)- PAR and Cd(II)- PAR is 518 and 409 nm, respectively Figure shows the 3D absorbance spectra of mixture of Pb(II) and Cd(II) after reaction with PAR at different concentration of Pb(II) and ... Simultaneous Spectrophotometric Determination of Pb(II) and Cd(II) Network trained with 20 epochs were suitable to be used in predicting the response of the co...
Từ khóa:
- determination of peaks troughs and elimination half life
- determination of critical depths and normal depths
- 3 determination of vc oxidants and other biochemical components
- prospects for high throughput structure determination of proteins by nmr spectroscopy
- — determination of required dimensions and material properties
- experimental determination of thermodynamic quantities and phase diagrams