Cloning, expression and characterization of oxysterol binding protein homologue 7 (OSH7) in yeast saccharomyces cerevisiae

... Oxysterol- binding protein 22 Yeast OSBP homologues 25 1.3.1 Structure of Osh proteins 25 1.3.2 Localization of Osh proteins 27 1.3.3 Function of Osh proteins 27 1.3.3.1 Role of OSH in maintaining ... Deletion of VPS4 causes redistribution of Osh7p 3.2.1 73 77 79 Osh7p-GFP shows different staining pattern in wild-type and vps4∆ strains 3.2.2 71 79 In vivo pro...

Ngày tải lên: 03/10/2015, 20:57

... 4779 Characterization of PDI from Conus marmoreus Z.-Q Wang et al A B Fig Comparison of amino acid sequences of PDIs from C marmoreus and other species (A) Multiple sequence alignment of PDIs from ... Catalysis of protein folding by protein disulfide isomerase and small-molecule mimics Antioxid Redox Signal 5, 413–424 Song JL & Wang CC (1995) Chaperone-like...

Ngày tải lên: 07/03/2014, 05:20

... usually consist of a tetrapeptide bearing the sequence YXXØ (Y is tyrosine, X is any amino acid, and Ø represents any hydrophobic residue) [36,37] The DPV gE protein contained YGSY and YNSL in ... production of polyclonal antibody Intervirology 2009, 52:141-151 12 Liu FY, Ma B, Zhao Y, Zhang Y, Wu YH, Liu XM, Wang JW: Characterization of the gene encoding glycoprotein C of...

Ngày tải lên: 12/08/2014, 04:20

... CLONING, EXPRESSION AND CHARACTERIZATION OF A NOVEL HELICOBACTER PYLORI DIFFERENTIATING ANTIGEN – HEAT SHOCK PROTEIN 20 DU RUIJUAN (B.Sc & M.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... 1.1 Helicobacter pylori and gastroduodenal diseases 1.2 Characteristics of Helicobacter pylori 1.3 Virulence factors of Helicobacter pylori 1.4 Hea...

Ngày tải lên: 12/09/2015, 10:19

... control of 30 s at 95 °C, 30 s at 50 °C and at 72 °C with a preheat at 95 °C and a 10 final extension at 72 °C using primers, S1 (5¢-GCAAATGCAACTGGA AGCGG-3¢) and A1 (5¢-ACAGCCTGCTAGCAAAGA GG-3¢) ... (5¢-ACAGCCTGCTAGCAAAGA GG-3¢) for amplification of HRT1, and primers S2 (5¢-GAAGAATCCTCTAAGGATAA-3¢) and A2 (5¢-TA CAAGGATTAATCCCTTGC-3¢) for amplification of HRT2 The PCR prod...

Ngày tải lên: 07/03/2014, 21:20

... exclusively of AATs from prokaryotes, including AATs from proto- Identification of a new aspartate aminotransferase zoa, archaebacteria and bacteria Interestingly, plants also have Ib subgroup-prokaryote-type ... mean ± SD of three determinations B subtilis B3 AATB3 Substrates L -aspartate L-glutamate a- ketoglutarate Oxaloacetate Fig Characterization of the purifi...

Ngày tải lên: 14/03/2014, 23:20

... sugars produced Michaelis constant (Km) and maximal rate (Vmax) values were calculated from a plot of the initial reaction rates vs substrate concentration using Kaleidagraph (SYNERGY Software) ... the data to the Michaelis-Menten equation All reducing sugar assays included a glucose standard curve The average molecular mass of the XG oligosaccharides (XGOs) was calculated from...

Ngày tải lên: 23/03/2014, 21:20

... aberrant expression of cathelicidins is often associated with various disease processes [26,27] In the present study, the gene cloning and characterization of three avian cathelicidin orthologs, ... Cc-CATH precursors from Coturnix coturnix, is reported, and the relationship between quail cathelicidins and other known vertebrate cathelicidins is analyzed Two of the t...

Ngày tải lên: 28/03/2014, 23:20

... this gene was designated naoA (nitroalkane-oxidizing enzyme gene) Expression of naoA in E coli To study the function of the naoA gene, it is necessary to obtain an adequate amount of NaoA protein ... recombinant NaoA after Sephadex G75 chromatography; lane 5, purified recombinant NaoA after DEAE-Sepharose Fast Flow chromatography; lane 6, standard molecular mass markers (phospho...

Ngày tải lên: 31/03/2014, 08:20

... Background Duck virus enteritis (DVE) is an acute and contagious disease of birds from the order Anseriformes (ducks, geese, and swans) [1-3] The causative agent of the DVE is Duck enteritis virus ... egress of Alpha-, Beta-, and Grammherpesviruses [8,14,16-20] However, there is no report on the identification and characterization of the UL31 gene produ...

Ngày tải lên: 12/08/2014, 04:21

... 498 nm bands and the shift of the band at 630 nm to the longer-wavelength direction At the same time, a broad band with the maximum at approximately 690 nm appears and increases with time The newly ... nm-band of NADPH decreases in proportion to the decrease of Soret band at 410 nm and to the increases of broad band spreading 600–700 nm The latter band was...

Ngày tải lên: 23/03/2014, 20:22

... doi:10.1186/1465-9921-12 -39 Cite this article as: Nikota et al.: Differential expression and function of breast regression protein 39 (BRP -39) in murine models of subacute cigarette smoke exposure and allergic airway ... the induction of BRP39 In contrast, BRP -39 was required for the expression of allergic airway inflammation Our study...

Ngày tải lên: 12/08/2014, 13:22

... process of soybean seeds as decreases in the mRNA levels of GmPDIL-1, GmPDIS-1 [26] and GmPDIM [27] were observed during the accumulation of the storage proteins Expression of certain seed storage proteins ... (2008) A novel plant protein disulfide isomerase family homologous to animal P5: molecular cloning and characterization as a functional protein f...

Ngày tải lên: 07/03/2014, 05:20

... of parallel b strands associated to an antiparallel strand (b2) and is surrounded by helices (a1 , a2 , a3 , a7 and a8 ) The second domain consists of helices a4 , a5 and a6 all clustered on the top ... indicating that Rv1399c is composed of 22% of a- helices, 25% of b-strand and 39% of random coil The catalytic triad is located at the bottom of a s...

Ngày tải lên: 07/03/2014, 16:20

... below for the biochemical analysis of the purified protein) Tissue- distribution and expression pattern of bovine tryptases Another interesting difference between the two bovine tryptase isoforms ... function of the two isoforms, prompted us to analyze the organization and the promoter sequences (Fig 4) of the two tryptase genes The length of both genes,...

Ngày tải lên: 17/03/2014, 09:20