Characterization of liver x receptor and retinoid acid receptor mediated response with transcriptome and histological analysis in zebrafish liver

... CHARACTERIZATION OF LIVER X RECEPTOR- AND RETINOID ACID RECEPTOR- MEDIATED RESPONSE WITH TRANSCRIPTOME AND HISTOLOGICAL ANALYSIS IN ZEBRAFISH LIVER HENDRIAN SUKARDI NATIONAL UNIVERSITY OF SINGAPORE ... Transcriptomic response to liver X receptor (LXR) agonist T0901317 in zebrafish liver 28 3.1 29 3.2 3.3 3.4 Histological analysis of T0...

Ngày tải lên: 03/10/2015, 20:32

... We interpret this observation as the retainment of newly synthesized proteins in the cytosol due to the inability to import matrix proteins by the fraction of growing and dividing glycosomes The ... lower (not shown) In order to determine the in uence of the reduction of the expression of TbPEX14 on the import of glycosomal matrix proteins,...

Ngày tải lên: 23/03/2014, 17:21

... space and spatial reckoning are inextricably culture- (and language-) bound, and a better understanding of space and the experience of living in space should be one of the primary goals of the ... Settlement and Urbanism: Proceedings of a Meeting of the Research Seminar in Archaeology and Related Subjects Held at the Institute of Archaeology, London Un...

Ngày tải lên: 11/06/2014, 16:57

... Plan of the paper We start by recalling in Section the principles of two classical methods dedicated to coefficient extraction in combinatorial generating functions, namely Darboux’s method and singularity ... at the singularity z = can be determined, resulting in (7) (The representation in (7) is in fact exact and not merely asymptotic It has been obtained by Zagier...

Ngày tải lên: 07/08/2014, 13:21

... mansoni NR1 A B W Wu et al DNA binding domain Ligand binding domain Fig Sequence alignment (A) Alignment of DNA binding domain (C domain) and its C-terminal extension (B) Alignment of ligand binding ... assay in in vivo results Experimental procedures Parasites The NMRI strain of S mansoni was maintained in snails (Biomphalaria glabrata) and Syrian golden hamsters (Mesoc...

Ngày tải lên: 07/03/2014, 11:20

... kept at °C and used within 10 days Quantification of alkaline phosphatase and aminopeptidase activities Specific alkaline phosphatase (ALP) and N-aminopeptidase (APN) enzymatic activities of BBMV ... sugar Canavalis ensiformis (ConA) a- Man a- Glc Galb1 fi 3GalNAc Galb1 fi 3,4GlcNAc a/ bGalNAc a/ bGal Galb1 fi 4GlcNAc Gala1 fi 3Gal GalNAca1 fi 3GalNAc GalNAca1 fi 3Gal Galb1 fi 3G...

Ngày tải lên: 23/03/2014, 13:20

... Gevaert et al COFRADIC and protein modifications analysis of purine-binding proteins in a total lysate of human Jurkat T-cells [40] primary separation mAU 1400 COFRADIC analysis of protein processing ... [34,36] and N-glycosylation [39] – and describe the use of COFRADIC for studying interactions between small molecules and proteins The latter is a particular applicatio...

Ngày tải lên: 18/02/2014, 16:20

... to the 9th cycle from the N-terminus including the initiating Met: MHKTHSTMS for Sno1p and MTGEDFKIKS for Snz1p Properties of the complex of Sno1p and Snz1p When Sno1p with a His-tag and Snz1p ... SNO3, SNZ2 and SNZ3 complement the defect The relationship of all of these genes remains to be clarified Expression and purification of Sno1p and Snz1p In light...

Ngày tải lên: 19/02/2014, 12:20

... blue, hematine, and p-chloranil were obtained from ICN; hydroxyethyl disulfide (HEDS) and vinylene trithiocarbonate was obtained from Aldrich EST identification and cDNA isolation The S mansoni EST ... (lane 4) electroblotted and immunodetected by a-SmGSTO serum (B) RT-PCR analysis of different S mansoni stages Reverse-transcribed RNA from miracidia (lane 1), sporocysts (lane...

Ngày tải lên: 21/02/2014, 01:21

... objective of understanding how the glycolytic ux in E histolytica is controlled, we cloned the genes, and overexpressed, puried and determined the kinetic parameters of the 10 glycolytic enzymes ... reconstruction of the nal stages of amoebal glycolysis Analysis of the kinetic properties of the recombinant glycolytic enzymes indicated that EhPPDK and EhPG...

Ngày tải lên: 07/03/2014, 17:20

... 16a-hydroxyprogesterone Both expressed enzymes had no lyase activity towards PROG or 17-OHPROG Understanding of the complexity of hydroxylase and lyase activity of CYP17 by way of primary sequence ... the metabolism of PREG by the two enzyme preparations Despite the large degree of homology between the human and baboon CYP17 the expressed baboon CYP17 was una...

Ngày tải lên: 08/03/2014, 09:20

... purification of the recombinant enzyme In order to construct an expression vector for Cl-idh, two oligonucleotides (sense, 5¢-AAAAACATATGGCAAGCA AATCGACCATCATCTACAC-3¢, and antisense, 5¢-AAA AAGGATCCCGGCTGAAAACCGGGCTGCATTA-3¢) ... dehydrogenase is known to predominantly catalyze the reduction of oxaloacetate to malate, and thereby lowering the possibilities of oxaloacetate ac...

Ngày tải lên: 08/03/2014, 10:20

... structure for the HS:19 serostrain and have shown that these labile groups are an a- l-sorbofuranose branch attached at C2 of b-d-GlcA and a MeOPN modification located at C4 of b-d-GlcNAc There are very ... the disappearance of signals associated with the sorbose monosaccharide and the production of the same oxidation product as observed for the l -sorbos...

Ngày tải lên: 16/03/2014, 13:20

... haemagglutinin at the C-terminus and encoding the cathepsin D propeptide fused to mature cathepsin E, we have demonstrated that the propeptide of cathepsin E, likewise those of other aspartic proteinases, ... maturation of cathepsin E, we constructed a mutant lacking the propeptide (Leu23-Phe58) and expressed it in HEK -293T cells The transfected cells were pul...

Ngày tải lên: 16/03/2014, 14:20