Effect of muscarinic agents on sclera fibroblast and their role in myopia 1

... examine the effect of atropine and pirenzepine (0 .1- 100 µM) on cell proliferation Fibroblasts were passaged from sclera and plated into 96 well plates containing DMEM with 10 %FBS Sclera fibroblasts ... reduction in BrdU incorporation (control: 1. 60±0.02, 0 .1 M: 1. 33±0.05, 1 M: 1. 18±0.06, 10 µM: 1. 13±0.04, 10 0µM: 1. 01 0.04, mean±s.e, p

Ngày tải lên: 17/09/2015, 17:20

... Fig 8A Effect of atropine inhibiting SF cell proliferation in culture Chick SF were incubated with atropine Phase contrast photomicrographs were taken after 24 hrs (magnification x100) A Control ... negative control Fig10B Protein corresponding to mAchR subtypes m1,m2,m4, and m5 identified in mouse sclera by immunoblotting Subtype selective antibodies bound to specific protein ban...

Ngày tải lên: 17/09/2015, 17:20

... inuence of anking bases on quadruplex stability and structural competition between the G -quadruplex and the duplex Results and Discussion To be able to assign a biologically relevant role to quadruplexes, ... nm excitation and 520 nm emission lters The wells were loaded with solutions of a xed concentration of preformed quadruplex (50 nm) and increasing concent...

Ngày tải lên: 07/03/2014, 02:20

... equilibrium of oxidation and chemical dissolution The dynamic equilibrium of the oxide formation (highly dependent on the acid concentration and availability of oxygen ions) and dissolution (relatively ... molecules once pass into one end (inner) of the pore would only come out from the other end (outer) of the pore without diffusing out from the middle of the pore- t...

Ngày tải lên: 19/03/2014, 16:48

... (Bui et aI., 2004) There were studies that only compare the effect of drying methods alone (Chan et aI., 2009); and also study on the optimization of extraction conditions on herbs (Chirinos ... 20 20 21 22 23 2.6 Effect of Extraction Methods on Antioxidant Activity of Herbs 2.6.1 Effect of Extraction Solvents 2.6.2 Effect of Extraction Time 2.6.3 Effect of...

Ngày tải lên: 05/05/2014, 08:39

... central laboratory in multicenter clinical trials: effect of ambient temperature on specimen temperature, and effects of temperature on mononuclear cell yield, viability and immunologic function ... apoptosis (Annexin V+, 7AAD-) and (B) late stages of apoptosis (Annexin V+, 7AAD+); (C) CD8 lymphocytes in early stages of apoptosis (Annexi...

Ngày tải lên: 18/06/2014, 19:20

... taken of the cranial tibialis (tibialis anterior) muscle and the cortical bone at pre-specified locations, namely, 5.0, 8.5, and 15.0 cm distal to the lateral tibial plateau The values were then averaged, ... recreating the same load level, load application time, and contact area for each animal Therefore, a standardized static load approach was used in this investig...

Ngày tải lên: 20/06/2014, 04:20

... taken of the cranial tibialis (tibialis anterior) muscle and the cortical bone at pre-specified locations, namely, 5.0, 8.5, and 15.0 cm distal to the lateral tibial plateau The values were then averaged, ... recreating the same load level, load application time, and contact area for each animal Therefore, a standardized static load approach was used in this investig...

Ngày tải lên: 20/06/2014, 07:20

... line is a guide to the eye The position of SO phonon down shifts with the decrease in diameter; (b) Dependence of the position of the SO phonon from the diameter of the NWs The points represent ... broadening of the phonon peaks As shown in Figure 2, beside LO and TO phonon peaks, there is another phonon peak centered around 230.0 cm-1, which can be att...

Ngày tải lên: 21/06/2014, 01:20

... 0.5 po 0.5 po 0.5 R Davis, B Lang / Journal of Retailing and Consumer Services 19 (2 012 ) 67–77 Game, Game, Game, Game, Game, Game, Game, Game, p 29 61. 16 (18 6) Game group X2/DF ratio 65.89 (24) ... coupon-prone consumer: some findings based on purchase behavior across product classes Journal of Marketing 51 (4), 99 11 0 76 R Davis, B Lang / Journal...

Ngày tải lên: 23/06/2014, 15:22

... 22 extraction yield (%) extraction yield (%) extraction yield (%) As shown in Fig 1A, ultrasonic power of 400 W is favourable for the extraction of the polysaccharides As shown in Fig 1B, the extraction ... Here, the value of R (0.9355) indicated good agreement between the experimental and predicted values of extraction yield of polysaccharides 3.1 Eff...

Ngày tải lên: 28/06/2014, 11:20

... effect of a wide spectrum of temperature-heat residence time combinations on the opening of P pinaster and P radiata cone scales, on the release of seeds, and on their viability We chose P pinaster ... affected the viability of the seeds enclosed in the cones in either of the species Therefore, it seems certain that cones provide effici...

Ngày tải lên: 30/06/2014, 05:04

... development of gluten Table Effect of fat type on the farinograph consistency of cookie dough Fat type Farinograph dough consistency (BU) (min) Bakery fat (Marvo) Margarine Hydrogenated fat (dalda) ... four type of fats on the rheology of the cookie dough and consequently on the quality of the cookies 2.2.2 Rheological characteristic of cookie...

Ngày tải lên: 07/07/2014, 20:58

... permission of the copyright owner Further reproduction prohibited without permission Reproduced with permission of the copyright owner Further reproduction prohibited without permission Reproduced ... permission of the copyright owner Further reproduction prohibited without permission Reproduced with permission of the copyright owner Further reproduction prohibited without...

Ngày tải lên: 09/07/2014, 14:21