Microfluidics and microarray based approaches to biological analysis 1
Microfluidics and microarray based approaches to biological analysis 1
... Kobayashi, M.; Horton, H.; Brown, E L Nat Biotechnol 19 96, 14 , 16 75 16 DeRisi, J L.; Iyer, V R.; Brown, P O Science, 19 97, 278, 680 17 Hacia, J Nat Genet 19 99, 21, 42 17 Chapter 18 Schena, M.; Shalon, ... target genes 1. 2.2 From Genomics to Proteomics 1. 2.2 .1 Limits of DNA Microarray- Based Strategies DNA microarray- based strategies allow for a detailed understand...
Ngày tải lên: 16/09/2015, 17:14
Microfluidics and microarray based approaches to biological analysis 5
... anti-phosphoserine and antiphosphotyrosine antibodies was developed Due to its sensitivity, high specificity and the short incubation time needed to detect phosphorylation, this antibody -based approach ... activities, and potentially other enzymatic activities The intercalation of various PEGs between the slide surface and the immobilized peptides minimized non-specific binding...
Ngày tải lên: 16/09/2015, 17:14
Microfluidics and microarray based approaches to biological analysis 4
... highlighted by its ability to withstand a variety of chemical conditions, which may make this new protein array compatible with most biological assays 4. 4 Materials and Methods 4. 4.1 Chemicals All chemical ... pH 7 .4, and spotted onto the avidin functionalized slides using an ESI SMA arrayer (Toronto, Canada) No incubation was necessary before the slide were further processed by...
Ngày tải lên: 16/09/2015, 17:14
Microfluidics and microarray based approaches to biological analysis 3
... a solution of 120 mM DIEA and 100 mM benzylmercaptan in DMF 94 Chapter 3. 3 .3 Array -Based Kinase Assay 3. 3 .3. 1 Spotting The N-terminally cysteine-containing peptides and N-terminally biotinylated ... washed, dried, scanned (Figure 3. 6) and the fluorescence intensity of the spots was measured (Figure 3. 7) 73 Chapter 3 mM mM 0 .3 mM 0.1 mM Figure 3. 6 Antibody -based fl...
Ngày tải lên: 16/09/2015, 17:14
Microfluidics and microarray based approaches to biological analysis 2
... SiOH Si O Si O CH2 + (MeO)3 Si Si CH3 CONH2 OMe (CH2)3 O (CH2)3 CH2 O CH2 O Si O Si CONH2 CH2 OMe (CH2)3 CH3 TEMED, Persulfate CH3 CH Si SiOH O C CH2 CH3 CH2 CH CONH2 Figure 2. 14 Reaction scheme ... 150 20 0 -6 -6.05 -6.1 -6.15 -6 .2 -6 .25 -6.3 Figure 2. 12 Ln(µ) as function of the number of base pairs (N) 40 25 0 Chapter u (*10E4cm2V1s-1) 1.9 1.85 1.8 1.75 1.7 1.65 1.6 1.55 1.9 2. 1...
Ngày tải lên: 16/09/2015, 17:14
Báo cáo khoa học: "Paragraph-, word-, and coherence-based approaches to sentence ranking: A comparison of algorithm and human performance" ppt
... paragraph as important, and the other sentences as not important We included this approach merely as a simple baseline 2.2 Word-based approaches Word-based approaches to summarization are based ... data Conclusion The goal of this paper was to evaluate the results of three different kinds of sentence ranking algorithms and one commercially available summarizer In order...
Ngày tải lên: 17/03/2014, 06:20
An effective trajectory based algorithm for ball detection and tracking with application to the analysis of broadcast sports video
... are based on the location and trajectory of the ball For example, touching of the ball (a player coming into contact with the ball physically), kicking of the ball, and passing of the ball These ... AN EFFECTIVE TRAJECTORY- BASED ALGORITHM FOR BALL DETECTION AND TRACKING WITH APPLICATION TO THE ANALYSIS OF BROADCAST SP...
Ngày tải lên: 15/09/2015, 21:56
Báo cáo y học molecular approaches to the analysis of deformed wing virus replication and pathogenesis in the honey bee, apis mellifera
... highly prevalent honey bee virus that causes wing deformity and mortality in honey bees worldwide, in both bees with wing deformity (symptomatic infection) and bees with normal wings (asympto- In ... of viruses in the honey bee, Apis mellifera J Invertebr Pathol 2006, 92:152-159 Chen Y, Pettis JS, Feldlaufer MF: Detection of multiple viruses in queens o...
Ngày tải lên: 20/12/2015, 08:30
Asset based approaches to poverty reduction moser 2
... introduction to asset- based approaches to poverty reduction in a globalized context The aim is to show the added value of asset- based approaches, in terms of both better understanding poverty and ... Bank 32 Appendix 1: Workshop Program Brookings Institution / Ford Foundation Workshop Asset- based Approaches to Poverty Reduction in a Globalized Context 27...
Ngày tải lên: 21/02/2014, 00:21
Báo cáo Y học: Characteristics of binding of insulin-like growth factor (IGF)-I and IGF-II analogues to the type 1 IGF receptor determined by BIAcore analysis pptx
... high-af®nity binding proteins (IGFBPs) with 10 -fold higher af®nity than their binding to IGF receptors IGFBPs thereby in¯uence the availability of IGF to bind to IGF receptors [12 ] Over the past 14 years, ... analogue binding to highanity recombinant IGF1 R Binding of IGF- II (A), des- (1 6) -IGF- II (B) and Arg6 -IGF- II (C) to rhIGF1R measure...
Ngày tải lên: 08/03/2014, 22:20
Báo cáo khoa học: The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis ppt
... either: (a) dsRNA; or (b) small interference (si )RNA The longer dsRNA may generate a large population of siRNA (with 21–23 nucleotides), and the use of longer dsRNA may be advantageous over siRNA ... corresponding to the mature peptide of MeMIH-B was amplified by PCR using T7 promoter-linked primers (forward, 5¢-TAATACGACTCACTATAGGTACTATG TATCGCATGCCAAT-3¢; reverse, 5¢-...
Ngày tải lên: 16/03/2014, 05:20
Marrying Prevention and Resiliency - Balancing Approaches to an Uncertain Terrorist Threat docx
... Nuclear, and Biological Terrorist Attacks, Santa Monica, Calif.: RAND Corporation, MR-1731-SF, 2003 12 Marrying Prevention and Resiliency: Balancing Approaches to an Uncertain Terrorist Threat ... iv Marrying Prevention and Resiliency: Balancing Approaches to an Uncertain Terrorist Threat Wall: Understanding Terrorist Efforts to Overcome Defen...
Ngày tải lên: 22/03/2014, 23:20
Urban Health and Society: Interdisciplinary Approaches to Research and Practice - Part 35 doc
... director, Institute on Urban Health Research, Northeastern University Urban Health and Society: Interdisciplinary Approaches to Research and Practice provides students in public health, urban ... approach to racial and, 135 136; research on health and, 21, 34–37, 143; urban food environments and, 47 Hurricane Katrina: destruction and deaths from, 224–22...
Ngày tải lên: 02/07/2014, 07:20
Urban Health and Society: Interdisciplinary Approaches to Research and Practice - Part 1 doc
... Foreclosure The Research and Its Context Focus Group Analysis and the Emergence of Health as an Issue vii 93 94 95 96 96 97 10 1 11 0 11 1 11 7 11 9 11 9 12 7 12 8 13 1 13 8 14 4 14 8 16 1 16 2 16 4 16 6 17 0 viii Contents ... Katrina September 11 , 20 01, Terrorist Attacks on New York City Implications for Prevention and Intervention Summary 18 3 18 5 18 6 18...
Ngày tải lên: 02/07/2014, 07:20
Urban Health and Society: Interdisciplinary Approaches to Research and Practice - Part 2 pps
... edited Urban Health and Society: Interdisciplinary Approaches to Research and Practice to prepare researchers and practitioners to be better equipped to meet the challenges of improving the health ... courses on interdisciplinary approaches to urban health for masters and doctoral students in public health and the social sciences, and collaborat...
Ngày tải lên: 02/07/2014, 07:20