3D domain swapping structural characterizations of domain sw

3D domain swapping structural characterizations of domain swapped dimer proteins FVE and rhodocetin

... heterodimer interface (Submitted) TABLE OF CONTENTS CHAPTER 1: Introduction 3D DOMAIN SWAPPING Background of 3D domain swapping 3D domain swapping definition Helpful definitions History of 3D domain ... domain swapping Diphtheria toxin More than one domain swapping Examples of 3D domain swapping Single mutation induce 3D domain swapping 11 De...

Ngày tải lên : 16/09/2015, 17:12

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Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modiﬁed protein and yields fragments with low immunoreactivity pptx

... gave the best results, both in terms of interpretation of the hydrolysis pattern and of reduced immunoreactivity [28] Trypsin and chymotrypsin were used in the present study, also in view of a ... namely the presence of the disulﬁde bridge connecting Cys66 and Cys160 in the native protein The position of these fragments in the primary sequence...

Ngày tải lên : 21/02/2014, 15:20

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Báo cáo khoa học: Structural studies of thymidine kinases from Bacillus anthracis and Bacillus cereus provide insights into quaternary structure and conformational changes upon substrate binding pot

... this study, we describe the structures of two homologues, TKs from B anthracis and B cereus, in complex with substrate dT and phosphate donormimicking dTTP Because of the high sequence identity ... expression and puriﬁcation of human thymidine kinase 1: quaternary structure, stability, and kinetics Protein Expr Purif 47, 506–515 20 Black ME & Hruby DE (1990) Quatern...

Ngày tải lên : 23/03/2014, 09:21

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Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx

... for the Ndh membrane subcomplex The Ndh antibodies recognized the 46, 28, 18 and 12 kDa polypeptides, corresponding to the NdhH, -K, -J and -E subunits of the Ndh complex The intact Ndh complex, ... et al of the ndh genes is much higher in BS chloroplasts, and elevated amounts of the Ndh complex have been found in these plastids [18] The functio...

Ngày tải lên : 23/03/2014, 13:20

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Báo cáo khoa học: " Evolutionary and structural analyses of alpha-papillomavirus capsid proteins yields novel insights into L2 structure and interaction with L1" ppsx

... domain of L2 to be conserved among the alpha-PVs irrespective of high or low risk group However, the structural interaction of L1 and L2 and formation of capsid is still not clear Minor capsid ... predict the secondary structure of L2 We also mapped the interaction domain of L2 within the monomer of L1 Our data shows that the amino terminus of L2 is i...

Ngày tải lên : 12/08/2014, 04:21

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Structural and binding characterization of the antiviral host proteins, VIPERIN and VAPC

... RESULTS AND DISCUSSION 2.3 Structure details of Viperin 2.3.1 Structural characterization of the human viperin fragments 42-45 2.3.2 Reconstitution of the [4Fe–4S] cluster in Viperin (45–361) and viperin ... 3.3.4.1 Structural characterization of VAPC4 3, VAPC3 1 and VAPC1 4 90-93 3.3.4.2 Interaction of VAPC4 3 with HCV NS5B 94-98 3.3.3.3 Interaction o...

Ngày tải lên : 09/09/2015, 18:57

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Structural analysis of calcium induced changes in gelsolin and adseverin

... disassembly Gelsolin/ villin superfamily severs actin filaments Twinfilin inhibits actin nucleotide exchange Actin/Bundling/Crosslinking Proteins α-actinin connects and organizes actin filaments EPLIN ... N-terminal half of gelsolin During apoptosis, caspase-3 cleaves gelsolin between domains and 4, yielding a calcium- independent, threedomain, actin-severing protein (Kothakota et...

Ngày tải lên : 11/09/2015, 10:17

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Tài liệu Báo cáo khoa học: Oligomerization of the Mg2+-transport proteins Alr1p and Alr2p in yeast plasma membrane pptx

... superfamily, Alr1p and Oligomerization of Alr1p and Alr2p Alr2p Overall sequence identity of these two proteins is 69% and exceeds 90% in the C-terminal part with its two predicted transmembrane domains ... Wachek et al Oligomerization of Alr1p and Alr2p Fig Alignment of transmembrane parts of CorA-related proteins and mutational alterations Alr1p,...

Ngày tải lên : 19/02/2014, 06:20

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Báo cáo khoa học: Functional dissection of two Arabidopsis PsbO proteins PsbO1 and PsbO2 doc

... amounts of an equimolar mixture of PsbO1 and PsbO2 were incubated with urea-washed PSII particles (Fig 4A,B), and the relative amounts of bound -PsbO1 2167 Functional dissection of PsbO1 and PsbO2 ... amount equivalent to lg of chlorophyll was loaded in each lane PsbO1 , PsbO2 ; mol PsbO1 or PsbO2 per PSII, : 1; mole PsbO1 and 1mole PsbO2 per PSII, :...

Ngày tải lên : 30/03/2014, 16:20

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Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

... bear structural similarity to the tight loop formed by L73–V78 of VpUcyt in the presence of DPC micelles Although the structural motifs adopted by the cytoplasmic domain of VpU appear to be qualitatively ... structure of the cytoplasmic domain of the human immunodeﬁciency virus type encoded virus protein U (Vpu) Int J Pept Protein Res 47, 297–...

Ngày tải lên : 18/02/2014, 06:20

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Báo cáo khoa học: Molecular basis of perinatal hypophosphatasia with tissue-nonspeciﬁc alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the crown domain potx

... hypophosphatasia, (b) infantile hypophosphatasia, (c) childhood hypophosphatasia, (d) adult hypophosphatasia and (e) odonto hypophosphatasia [1–4] Perinatal and infantile forms of hypophosphatasia are ... Molecular basis of perinatal form of hypophosphatasia N Numa et al Hypophosphatasia is caused by various mutations of the tissue-nonspeciﬁc alkaline pho...

Ngày tải lên : 07/03/2014, 05:20

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Báo cáo khoa học: Structural mobility of the monomeric C-terminal domain of the HIV-1 capsid protein pptx

... Ser146 in the numbering of the intact CA) The CACW40A protein is monomeric, and its structure is similar to that of the subunits in the dimeric, non-mutated CAC, but, in the monomeric form, the segment ... responsible, from a thermodynamic point of view, for the binding of the monomeric species of CAC? We have previously discussed the variation in the fr...

Ngày tải lên : 07/03/2014, 06:20

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Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy pot

... structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also to study the effect of thermal unfolding in the presence and the absence of MgATP We used 2D correlation spectroscopy ... degree of homology ( 80%) with the 3280 catalytic domain of other isoforms of PKC belonging to the classical or novel subfamilies...

Ngày tải lên : 16/03/2014, 13:20

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Báo cáo khoa học: Characterization of the dimerization process of a domain-swapped dimeric variant of human pancreatic ribonuclease pdf

... for the putative mechanism of domain-swapping dimerization of RNase A and the human pancreatic ribonuclease variant, PM8 For RNase A (A) , the protein is subjected to conditions that favour the ... chemical baselines Cpex, expression of the partial heat capacity of the protein relative to the heat capacity of the protein in the native state the monom...

Ngày tải lên : 16/03/2014, 13:20

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Báo cáo khoa học: Structural basis of p63a SAM domain mutants involved in AEC syndrome ppt

... the SAM domain family SAM domains, in general, were initially thought to be involved in protein–protein interactions by forming homo- or heterooligomers with other SAM domains or by interacting ... conserved cysteines (C558 and C561) in p63a SAM domain are also reduced as in the p73a SAM domain The inclusion of a reducing agent in the crystallization of p63a...

Ngày tải lên : 22/03/2014, 16:20

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