Cloning and characterization of diamine oxidase and glutamate decarboxylase genes of mustard (brassica juncea) and their roles in shoot morphogenesis in vitro
... CLONING AND CHARACTERIZATION OF DIAMINE OXIDASE AND GLUTAMATE DECARBOXYLASE GENES of MUSTARD (BRASSICA JUNCEA) AND THEIR ROLES IN SHOOT MORPHOGENESIS IN VITRO JIAO YUXIA (M.Eng ... involved in shoot regeneration in vitro This was achieved by the cloning and characterization of diamine oxidase (DAO) and glutamine decarboxylas...
Ngày tải lên: 12/09/2015, 10:19
... ISOLATION AND MOLECULAR CHARACTERIZATION OF GENES ASSOCIATED WITH SHOOT REGENERATION OF MUSTARD (BRASSICA JUNCEA) IN VITRO GONG HAIBIAO (M.Sc SJTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR ... of genes associated with shoot regeneration from leaf disc explants of mustard (Brassica juncea) in vitro Discussion 84 The cloning of...
Ngày tải lên: 17/09/2015, 17:19
... fabrics and on the washing machine’s heating elements - stabilise alkalinity at the correct level throughout the washing process thus giving good soil removal and enabling the other components of the ... present in the washing machine from previous washes, thus reactivating any remaining detergents and improving detergent performance No other single chemical...
Ngày tải lên: 11/07/2014, 16:10
The ancient origin of NF kb ikb and thioredoxin and their roles in immune response
... considerations, the original function of the NF- κB signaling pathway was the activation of innate immune responses Indeed, the function of IKK and NF- κB in the fruit fly is in the activation of innate immune ... expression of multiple inflammatory and innate immune genes The proinflammatory cytokines IL-1β and TNF-α activate NF- κB, and their e...
Ngày tải lên: 14/09/2015, 22:25
Báo cáo khoa học: Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis doc
... organism for cloning the genes encoding the different fatty acid desaturases involved in EPA biosynthesis and identified two sequences coding for fatty acid desaturases with D5- and D6-regioselectivities ... contains 12 acidic amino acids (aspartate and glutamate), whereas the corresponding domain sequences in PtD5p and PtD6p have only eight and five acidic residues,...
Ngày tải lên: 31/03/2014, 09:20
Cloning, expression and characterization of oxysterol binding protein homologue 7 (OSH7) in yeast saccharomyces cerevisiae
... Oxysterol- binding protein 22 Yeast OSBP homologues 25 1.3.1 Structure of Osh proteins 25 1.3.2 Localization of Osh proteins 27 1.3.3 Function of Osh proteins 27 1.3.3.1 Role of OSH in maintaining ... Deletion of VPS4 causes redistribution of Osh7p 3.2.1 73 77 79 Osh7p-GFP shows different staining pattern in wild-type and vps4∆ strains 3.2.2 71 79 In vivo pro...
Ngày tải lên: 03/10/2015, 20:57
Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
... mansoni NR1 A B W Wu et al DNA binding domain Ligand binding domain Fig Sequence alignment (A) Alignment of DNA binding domain (C domain) and its C-terminal extension (B) Alignment of ligand binding ... assay in in vivo results Experimental procedures Parasites The NMRI strain of S mansoni was maintained in snails (Biomphalaria glabrata) and Syrian golden hamsters (Mesoc...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx
... Temperature-dependent ricin A chain mutants Fig Stability of mutant ricin A chains The kinetics of protein degradation of (A) Kar2SP-RTAE177D and (B) Kar2SP-RTAF108L ⁄ L151P at all temperatures, or a cytosolic ... 5¢-ATATTCCCCAAACAATACCC-3¢ and the antisense primer CP133 5¢-TTAAAACTGTGACGATGGT GGA-3¢ with the TAA termination anticodon shown in bold Amplification react...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Expression and characterization of cyanobacterium heme oxygenase, a key enzyme in the phycobilin synthesis Properties of the heme complex of recombinant active enzyme potx
... 498 nm bands and the shift of the band at 630 nm to the longer-wavelength direction At the same time, a broad band with the maximum at approximately 690 nm appears and increases with time The newly ... nm-band of NADPH decreases in proportion to the decrease of Soret band at 410 nm and to the increases of broad band spreading 600–700 nm The latter band was...
Ngày tải lên: 23/03/2014, 20:22
Báo cáo khoa học: Identification and structural characterization of a sialylated lacto-N-neotetraose structure in the lipopolysaccharide of Haemophilus influenzae pptx
... treatment with neuraminidase (as indicated by – and +, respectively) The sialylated tetrasaccharide-containing glycoforms are indicated by an asterisk All strains were grown on media containing ... that a terminal Sial-lNnT unit is linked to the Hep I residue of the LPS inner core in the sialylated glycoform The structure of the sialylated glycoforms of the O...
Ngày tải lên: 23/03/2014, 21:21
Báo cáo khoa học: Characterization of recombinant prolidase from Lactococcus lactis – changes in substrate specificity by metal cations, and allosteric behavior of the peptidase pdf
... understanding of the characteristics of this peptidase, which would be of industrial use in the debittering of fermented foods Results and Discussion Cloning and expression of Lc lactis prolidase The prolidase ... protein engineering of this enzyme In the present study, the prolidase- coding gene, pepQ, was isolated from Lactococcus lactis NRRL B-18...
Ngày tải lên: 30/03/2014, 04:20
Báo cáo y học: "Prevalence, clinical relevance and characterization of circulating cytotoxic CD4+CD28– T cells in ankylosing spondylitis" ppt
... protein that is expressed in the cytoplasmic granules of cytotoxic T cells and NK cells, providing them with the ability to lyse target cells Thus, CD4+CD28– T cells are distinct from classic T ... between the levels of CD3 +CD4+CD28– T cells and grade of clinical restriction in ankylosing spondylitis patients Patients were grouped into those with minor...
Ngày tải lên: 09/08/2014, 01:23
Báo cáo y học: "Structural and functional characterization of human apolipoprotein E 72-166 peptides in both aqueous and lipid environments" pot
... concentration of DHPC Protein -lipid interactions and Protein-LDLR binding of ApoE- (72-166) Proteins To identify and compare the lipid binding ability of the three apoE- (72-166) peptides, we assessed ... Received: 17 September 2010 Accepted: 10 January 2011 Published: 10 January 2011 References Weisgraber KH, Rall SC Jr, Mahley RW: Human E apoprotein heterogeneity Cysteine...
Ngày tải lên: 10/08/2014, 05:21
Báo cáo y học: " Characterization of protein tyrosine phosphatase H1 knockout mice in animal models of local and systemic inflammation" docx
... processing of other cytokines and cytokine receptors [32-35] Interestingly, PTPH1 is known to inhibit TACE expression and activity in vitro [36] We therefore analyzed cytokines plasma levels in carrageenan-treated ... Clin Pathol 2008, 129:735-743 doi: 10.1186/1476-9255-7-16 Cite this article as: Patrignani et al., Characterization of protein tyrosine phosphatase H1 kno...
Ngày tải lên: 11/08/2014, 03:20