Structural investigations of redox regulation in ATFKBP13 4

... experiments to determine the interacting domains of the AtFKBP13 and the Rieske proteins A number of cDNA fragments encoding various domains of the AtFKBP13 or Rieske protein were fused in frame with ... process 4. 2 .4 Mature FKBP13 is a target for reduction by thioredoxin The possibility of thioredoxin-linked reduction of AtFKBP13 arose from the finding of two solvent...

Ngày tải lên: 16/09/2015, 15:55

... Reflections (working/test) 40 ,30 9/4, 530 40,687/4,569 0.21 / 0. 23 0.20 / 0. 23 Non-hydrogen atoms 4, 630 4, 630 Waters 4 63 3 13 Protein 21.497 32 .36 7 Waters 32 .060 39 .37 9 R.M.S.D in bond lengths (Å) ... 3. 8.2 Crystallization of AtFKBP 13- (SH)2 Crystals of reduced AtFKBP 13 [AtFKBP 13- (SH)2] were produced in the same way as the oxidized AtFKBP 13 crystals T...

Ngày tải lên: 16/09/2015, 15:55

... processes diurnally in an effective manner The ferredoxin/thioredoxin system—composed of ferredoxin, ferredoxin-thioredoxin reductase and thioredoxin— facilitates these redox changes in the target ... SH3 domains It is possible that immunophilin-like domains will be found in other proteins not thought of as immunophilins In this vein, it is interesting to note that the scaffoldi...

Ngày tải lên: 16/09/2015, 15:55

... more flexibility, both of the backbone of the protein and of the spacing between particular residues [Bowie et al., 19 91] Each residue of a protein is assigned one of 18 environment classes, ... crystallography Instead of stereochemical parameters, Sippl (19 93) uses knowledge of the forces, which stabilize proteins in solution in the analysis of the energy distribution...

Ngày tải lên: 16/09/2015, 15:55

... before hypoxia to further confirm the mechanism of BSO modulating the Page of expression of HIF-1a by the changes of micro-environment redox status in the cells Intracellular GSH assay After the ... considered the mechanism, the redox status influencing the expression of HIF1a, as following: (i) The biosynthesis of GSH impose a reducing micro-en...

Ngày tải lên: 10/08/2014, 10:21

... but rather a vital part of the war effort; they turned lipstick into a symbol of resilient femininity in the face of danger, a symbol that would boost the morale of both the women wearing the lipstick ... sides of the ocean.238 Max Factor developed the first truly indelible lipstick, in the sense of long-lasting rather than of permanent, titled Tru...

Ngày tải lên: 16/01/2014, 22:20

... residue in the N-terminal part of the peptide that binds in the with the C-terminal domain in CaM However in PhK5 Trp357 is found at the C-terminal end of the peptide This suggests that either PhK5 ... occurring on binding of the peptide and could indicate that the binding of PhK5 to Ca2+⁄ CaM is similar to that observed for Ca2+⁄ CaM binding to i...

Ngày tải lên: 19/02/2014, 17:20

... [104,105] and in S cerevisiae [88] have lead to a profound understanding of how DNA binding, inducer binding and dimerization function in TetR This informa- Gene regulation by tetracyclines (Eur ... concentrations of tc for full induction [15] These conflicting requirements are met by the genetic organization of the resistance determinants (reviewed in [6]) and...

Ngày tải lên: 23/03/2014, 21:20

... harmonics information contained in the audio signals In the feature extraction, we proceed in two stages as indicated by Figure In the first stage, we use a small analysis window to investigate how the ... sequence of length N = 901, corresponding to 15 minutes of audio Tripling the length N by using a threefold concatenation of the Bolero results in a running...

Ngày tải lên: 22/06/2014, 23:20

... the absence of Campylobacter infection in 75 beavers examined in North America Salmonella contamination and salmonellosis in humans and farm animals is an increasing problem in the industrialized ... Salmonella were detected in any of the samples examined We conclude that the beaver does not seem to be involved in drinking water contamination with these pathogens in Tel...

Ngày tải lên: 12/08/2014, 15:20

... many We then analyzed the histories of individual regulatory interactions To determine whether gene regulation evolves by duplication, we examined the evolutionary histories of regulatory interactions ... orthologous genes in E coli and its relatives, they did not examine the regulation of recently acquired genes As most of the genes in E coli K12 were acquired by...

Ngày tải lên: 14/08/2014, 08:20

... Sensitivity of ModuleMiner' s performance to the quality of the input genes Sensitivity of ModuleMiner' s performance to the quality of the input genes The ratio of true positive genes (containing the ... ranking new target genes of the cardiac muscle TRGM were MYL3 ('cardiac myosin light chain 1'), MYOD1 ('myoblast determination protein 1'), TNNI1 ('troponin I'), and MYH3 ('m...

Ngày tải lên: 14/08/2014, 08:21

... domains 19 DNA-binding Domains In accord with the sequence and structure of DNA-binding domains, regulatory proteins are often grouped into class The goal function of the DNA-binding domain rules ... Methods to derive principles of transcription regulation 21 3.1 Principles of transcription regulation 21 3.2 Typical methods to derive principles of transcription r...

Ngày tải lên: 25/03/2015, 09:45

... generation of thermoelectric materials is going on One approach is using new categories of advanced bulk thermoelectric materials [7-9] They are so-called ‘phononglass electron-crystal’ (PGEC) materials, ... calculate the thermoelectric properties The results of this thesis should reveal the physical insights of these advanced low- dimensional thermoelectric mater...

Ngày tải lên: 09/09/2015, 11:25

... modestum subunit c in chloroform/methanol/water (4 : : 1) Samples of mM unlabelled subunit c of Propionigenium modestum in C2 HCl3 /C2 H3OH/H2O (4 : : 1), 25 mM D11-Tris/HCl were prepared to test the ... helices in the structure in chloroform/methanol/water (4 : : 1) [23], amino-acid sequence of P modestum subunit c...

Ngày tải lên: 08/03/2014, 10:20