Structural basis for recruitment of traffic proteins by small GTPases

... STRUCTURAL BASIS FOR RECRUITMENT OF TRAFFIC PROTEINS BY SMALL GTPASES WU MOUSHENG (B.Sc., M.Sc) Xiamen University A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL ... regulations Structural and biochemical studies of small GTPases showed that there are two interconvertible forms of small GTPases: a GDP-bound inactive f...

Ngày tải lên: 16/09/2015, 08:31

... Professor Jerzy Ciesiolka for critically reading the manuscript and for helpful FEBS Journal 275 (2008) 1651–1662 ª 2008 The Authors Journal compilation ª 2008 FEBS Recognition of Co2+ by RNA aptamers ... an alternative structure of the aptamer cannot be excluded However, FEBS Journal 275 (2008) 1651–1662 ª 2008 The Authors Journal compilation ª 2008 FEBS 1653 Recognition...

Ngày tải lên: 16/03/2014, 06:20

... further oligosaccharides that are fragments of NA2F The structural information is complemented by thermodynamic parameters of binding determined by means of isothermal titration calorimetry for ... second conformation of the side chain of Glu221, which also adopts two conformations but not correlated with the anomeric form of the bound mannose is shown in dark gr...

Ngày tải lên: 23/03/2014, 11:20

... understanding of how interactions could take place in different regions of the molecule As part of a long-term function-oriented structural effort aimed at understanding the molecular bases of polyQ ... molecular bases of polyQ disease proteins, we report here a study of the structural determinants for the interactions of Anp32a with other partners As a prerequisite...

Ngày tải lên: 18/02/2014, 17:20

... sugar-binding proteins should be defined in terms not of the affinities for sugars but of whether the protein adopts the open form or the closed form TvuCMBP–c-CD complex is seen as the closed form (Fig ... sugar-binding protein family was found to be located upstream of the TVA II gene, and the affinities of this protein for c-CD were higher than that for maltose [7] The results sug...

Ngày tải lên: 19/02/2014, 00:20

... Stereoview of the RMD monomer The cofactor-binding domain and the substratebinding domain are shown in aqua and light sand, respectively; the APPR portion of the cofactor (dark gray) and the ligand ... disease and reduced lung function [20] An eight-gene cluster encodes functions for synthesis and export of A-band O polysaccharide [21,22], and contains genes...

Ngày tải lên: 16/03/2014, 01:20

... structures of VioC hydrogen bond to the peptide group of Ser158 In addition, the guanidinium group of the l-arginine side chain forms salt bridges to the closely adjoined side chains of the acidic ... by the hydroxyl group of the side chain of Ser224 and the carboxamide group of the side chain of Gln137 Gln137 is suitably oriented to interact with the...

Ngày tải lên: 16/03/2014, 02:20

... with the symmetry of space group C2221 Overall hydrophobic NNRTI interactions to wild-type and K103N mutant RT The interactions of Efavirenz, MSC194 and PNU142721 to wild-type RT and the K103N mutant ... study we present the structural indications for the role of K103 and N103 in drug binding and the structural implications for the inhib...

Ngày tải lên: 18/03/2014, 01:20

... structure) for cocrystallization of E chrysanthemi GH30 xylanase Fig S2 Dynamic light scattering profile of E chrysanthemi GH30 xylanase Fig S3 Examples of distinct habits of E chrysanthemi GH30 xylanase ... three-dimensional structure of the E chrysanthemi GH30 enzyme [16], together with its established mode of action [14], allowed us to present a hypothesis for the basis...

Ngày tải lên: 22/03/2014, 16:20

... comparing the phosphorylation of the natural substrates of the double mutant with the single mutant, it seems that the mutation N45D in the double mutant counteracts the negative effect(s) of the N64D ... bound to the 3¢-OH group of the substrate while there is no density for that interaction in the mutant structure (Fig 2B) Structural basis for...

Ngày tải lên: 30/03/2014, 20:20

... during the assembly of the head of bacteriophage T4 Nature 227, 680–685 43 Ogawa, K., Tashima, M., Yumato, Y., Okuda, T., Sawada, H., Okuma, M & Maruyama, Y (1990) Coding sequence of human placenta ... were able to demonstrate that this was the case for both ADP and ATP–actin (not Fig Effect of pH on the interaction of actin with cofilin (A) Effect of pH on the interact...

Ngày tải lên: 31/03/2014, 09:20

... Reactions catalyzed by CSE CSE converts L-cystathionine into L-cysteine, α-ketobutyrate and ammonia in the reverse transsulfuration pathway via an α,γ-elimination reaction This enzyme can also ... non-active CSE mutations are associated with cystathioninuria, a disease characterized by accumulation of cystathionine in blood, tissue and urine, and 20 sometimes associated with m...

Ngày tải lên: 11/09/2015, 10:17

... DEVELOPING SOFTWARE TOOLS FOR STRUCTURE DETERMINATION OF LARGE PROTEINS BY NMR SPECTROSCOPY ZHANG LEI B.SC (HONS.), NUS A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE GRADUATE ... cycle of refinement may go on several times before a high resolution structure can be determined Figure 1.5: Outline of the procedure for protein structure determinat...

Ngày tải lên: 04/10/2015, 15:45

... contribute to the susceptibility of E coli model membranes to the action of AP1 An important determinant of the susceptibility of membranes to a-AMPs is the packing characteristics of the individual ... hypothesis of Tytler et al [48], it may be that insertion of the inverted wedge shape formed by the AP1 a-helix into membranes of E coli leads to the...

Ngày tải lên: 07/03/2014, 12:20

... and antisense strand (5¢-ATGAGTTTTCCAGAGCAACTT-3¢), and siRNA was synthesized using DNA templates (sense strand, 5¢-AAATGAGTTTTCCAGAGCAACCCTGTCTC-3¢; antisense strand, 5¢-AAGTTGCTCTGGAAAACTCATCCTG ... TTCTGGCCTTC-3¢, and that of s -DNA is 5¢-ATAGGCG GGAATTTTGCATC-3¢ Anti-TMV siRNA was synthesized using the DNA templates 5¢-AAGGGACGAGCA TATGTACACCCTGTCTC-3¢ and 5¢-AAGTGTACATAT G...

Ngày tải lên: 16/03/2014, 02:20