Development and application of advanced proteomic techniques for high throughput identification of proteins

... DEVELOPMENT AND APPLICATION OF ADVANCED PROTEOMIC TECHNIQUES FOR HIGHTHROUGHPUT IDENTIFICATION OF PROTEINS HU YI (B.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... analysis of proteins, i.e proteomics (Pandey and Mann, 2000; Tyers and Mann, 2003) Therefore, the advancement of proteomics relies largely on the development...

Ngày tải lên: 16/09/2015, 08:30

... focused on the analysis of DNA fragments; and the second part (chapter and chapter 5) focused on the analysis of organic pollutants Several novel capillary electrophoresis (CE) techniques had ... various applications, the CE applications in DNA analysis and monitoring of pollutants are of significant importance 1.3.1 CE Application in DNA Analysis...

Ngày tải lên: 14/09/2015, 11:40

... biomarkers, and they participated in the data analyses AAH developed the customized Spotfire tool used for data analyses and reviewed statistical analyses YG participated in the assessment and ... assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood Journal of Translati...

Ngày tải lên: 18/06/2014, 16:20

... (and Lys) tend to be over- High density IJISS DNA arrays have been developed for global analysis of Streptomyces gene expression and transcription factor binding The HspR regulatory system of S ... against the test arrays and the probe performance was scored using the following equations Firstly the Cy3 and Cy5 background-subtracted signals, designated 'g' an...

Ngày tải lên: 14/08/2014, 21:20

Ngày tải lên: 14/11/2014, 12:55

... of influenza A (H1N1) virus infection to explore the impact of influenza infection and inflammation on DNA damage and DNA damage responses By studying chromatin phosphorylation as a measure of DNA ... of DNA damage, we show that the level of DNA damage increases following influenza infection, and we provide data that supports a role for replication fork breakdow...

Ngày tải lên: 09/09/2015, 08:16

... αglucosidase and α-amylase can significantly reduce the post-prandial increase in blood glucose after a meal, with starch as the major calorie intake The apparent advantage of the starch hydrolase inhibitors ... linear range of gelatinized starch Table Accuracy and precision of the high- throughput method Table IC50 and acarbose equivalence of inhibitors obtaine...

Ngày tải lên: 10/09/2015, 09:01

... develop and apply computational methods and tools for ADR and toxicity prediction The methods developed in this work are potentially useful for development and application of QSAR models as well ... complex, time consuming and resource intensive Toxicity is one of the primary reasons for the failure of drug candidates in later stages of drug deve...

Ngày tải lên: 10/09/2015, 09:02

... differentiation, and to the design of disease- targeting molecules like small-molecule agents or antibodies for disease treatment The key disease markers determine the characteristics of disease, therefore ... effectiveness of our system on deriving stable disease markers and discovering therapeutic target One major application of marker discovery is the find...

Ngày tải lên: 12/09/2015, 08:20

... Three -phase hollow fiber- protected liquid- phase microextraction techniques combined with HPLC analysis 42 2.1 Orthogonal array designs for the optimization of static three -phase hollow fiber- protected ... the hollow fiber This thesis reports on the development and application of hollow fiber- protected LPME techniques to trace organic analysis...

Ngày tải lên: 12/09/2015, 08:20

... domain finite methods for the numerical solution of time dependent Maxwell’s equations The two finite methods focused are FDTD and FETD methods The applications of the developed hybrid methods are ... analysis of passive microwave circuits and studies in dosimetry and tissue interaction CHAPTER TIME DOMAIN FINITE METHODS FOR SOLUTION OF MAXWELL’S EQU...

Ngày tải lên: 14/09/2015, 11:39

... drop -in- drop system The aqueous phase of the outer drop contained the analyte of interest and was continuously delivered and aspirated away throughout the sampling The analytical response of the instrument ... involves the use of hollow fiber combination with liquid- phase microextraction It can be categorized into two- ii phase microextraction, and thr...

Ngày tải lên: 17/09/2015, 17:20

... Investigation of DNA damage as a potential mechanism of influenza pathogenesis 66 3.1.2 Phosphorylation of H2AX (Ser-139) during DNA strand breaks 69 3.1.3 DNA damage is triggered by influenza ... to sense for DNA damages, activate cell signaling and repair DNA lesions DNA damage are surveyed via DNA damage sensors, Mre11-Rad50-Nbs1 (MRN) complex, and re...

Ngày tải lên: 30/09/2015, 06:20

... spectrum of BT5AA 56 Synthesis and mass spectrum of 2TAA 58 Synthesis and mass spectrum of 3TAA 59 Synthesis and mass spectrum of 5M2TAA 60 Synthesis and mass spectrum of ... from the ancient remains of marine plant and animal life under extreme heat and pressure in an anaerobic environment Depending on the composition of the organic material, varying proport...

Ngày tải lên: 13/05/2014, 14:01

... irradiated at 14 0 oC for 40 Figure 2 -1 Library of 2 -1- 16, 2 -1- 17, 2 -1- 18, 2 -1- 19, 2 -1- 20 32 2.2.2.3 Solid-Phase Synthesis of 2 -1- 16, 2 -1- 18, 2 -1- 19 and 2 -1- 20 To illustrate the versatility of this ... process 10 Figure 1- 8 Safety-catch Linkers 12 Figure 2 -1 Library of 2 -1- 16, 2 -1- 17, 2 -1- 18, 2 -1- 19, 2 -1- 20 31 Figure 3 -1 Polymer-supported Han...

Ngày tải lên: 11/09/2015, 10:15