Investigation and characterization of splice variations of l type ca2+ channel, cav 1 3, in chick basilar papilla and rat cochlea hair cells iimplications in hearing

... whole chick basilar papilla 70 3 .11 Detection of Cav1 .3IQD splice variant in single cell RT-PCR of chick hair cells 72 3 .12 Relative abundance of Cav1 .3IQD splice variant in developing chick cochlea ... of Cav1 .3IQ splice variants identified at C-terminus of chick basilar papilla 71 Summary of Cav1 .3IQ splice variants in individual h...

Ngày tải lên: 14/09/2015, 18:17

... proteins from B subtilis and E coli Results and Discussion NadA cloning and protein purification and characterization In order to optimize the heterologous production and purification of B subtilis ... enzyme from E coli [9], NadB from B subtilis is a dimer of 115 kDa in the absence of NaCl and a monomer of 55 kDa in the presence of 150 mm NaCl After incubati...

Ngày tải lên: 30/03/2014, 02:20

... SINGLE- CHANNEL AND WHOLE- CELL ELECTROPHYSIOLOGICAL CHARACTERIZATIONS OF L- TYPE CaV1. 2 CALCIUM CHANNEL SPLICE VARIANTS: RELEVANCE TO CARDIAC AND NERVOUS SYSTEM FUNCTIONS PETER BARTELS -Diploma ... advantages of the cell attached mode of single- channel recordings in relation to the whole cell technique 1.5 Single- channel vs whole...

Ngày tải lên: 08/09/2015, 19:30

... Purification of four pulchellin isoforms from A pulchellus seeds Using a combination of affinity, ion exchange and chromatofocusing chromatography, four pulchellin isoforms were isolated from A pulchellus ... II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform (P I, P II, P II and P IV) The A-chain of...

Ngày tải lên: 18/02/2014, 16:20

... Fig Structural comparisons of the funnels of LGOX, LAAO, and PAO, and the residues composing two entrances of the funnel of LGOX (A) Stereo view of funnels of LAAO, PAO, and LGOX The surfaces of ... quantitative assay of l-glutamate is important in the fields of food production and clinical biochemistry Actually, l-glutamate oxidase (LGOX; EC 1.4.3.11) was first purifie...

Ngày tải lên: 23/03/2014, 05:22

... 17-fold enhancement of GL binding to the wild-type upon the addition of the same concentration of phosphate pH profiles The pH dependences of logarithmic rates of the H334G mutant and wild-type are ... wild-type and with an optimum pH of 6.5 A shift of the pH profile of about + 0.5–1.0 pH units and an optimum pH similar to that of the wild-type wa...

Ngày tải lên: 23/03/2014, 07:20

... phosphorylation of GST–TSSK3 with [32 P]ATP[cP] The phosphorylation reactions of GST–TSSK3K39R by Myc-PDK1 [32 ] immunoprecipitated from 293T cells and of HA–TSSK3K39R immunoprecipitated from 293T cells, by ... maximum activation and ⁄ or activation by PDK1 of TSSK3, as suggested for PKCf phosphorylation and activation by PDK1 [35 ] Thus we hypothesize that in orde...

Ngày tải lên: 23/03/2014, 11:20

... human and a murine NICN1 cDNA clone from the IMAGE collection and determined the complete sequences of these cDNAs The comparative analysis of the human, canine, and murine NICN1 cDNA revealed a ... CACCAGgtcagctgggcctca 423 418 bp (exon 3, 11 4 bp) … CCAAAGgcaagtgactttgca 4 01 bp (exon 4, 72 bp) 495 … CTTGAGgtaagctctctaaca 3 71 bp (exon 5, 10 5 bp) 600 … TTCGACgtgag...

Ngày tải lên: 23/03/2014, 21:20

... theophylline The arrow points to the M-A2aTr316-H10 fusion protein Note that the main contamination, seen in lane 1, runs only marginally above the hA2aR fusion protein and is the main band in ... obtained, namely LysIle-Glu-Glu-Gly-Lys-Leu-Val-Ile-Trp corresponds to the N-terminus of the mature maltose-binding protein Binding of the fusion protein to the IMAC gel...

Ngày tải lên: 24/03/2014, 00:21

... Lepidoptera, the treatment of adults with the ACE inhibitor, captopril, causes a decrease in egg-laying [9] In Haematobia irritans exigua, a blood meal initiates the strong synthesis of ACE in the ... size increase In the resting period, the vas deferens are atrophied and are barely visible At the cellular level (Fig 4A) , the testis is composed of acini...

Ngày tải lên: 30/03/2014, 01:20

... strain to those of the other simian rotavirus strains, SA11 and RRV Materials and methods Rotavirus isolation The YK-1 strain of simian rotavirus was isolated from the diarrheal stool of a 2-year-old ... was based on a Jennerianapproach prompted by studies indicating that animal and human rotaviruses share a common group antigen and that experimental animals immunize...

Ngày tải lên: 20/06/2014, 01:20

... Morrey- type Besov and Triebel-Lizorkin spaces, which is a characterization of Morrey- type Besov and Triebel-Lizorkin spaces Before stating it, we recall some notations and the definition of Morrey- type Besov ... < p = q < ∞, and β ∞, then M Bq,q = Bq,β s,β s and M Fq,q = Fq,β , standard Besov and Triebel-Lizorkin spaces respectively; see [22] A Cha...

Ngày tải lên: 06/08/2014, 05:20

... Pellets of single phase sample were prepared using a stainless steel die measuring mm in diameter Sufficient amount of powder was added, cold pressed uniaxially, and sintered at 1100oC in order to increase ... its volatile, reactive characteristic and relatively low firing temperature in forming binary or ternary materials with other elements, e.g bismuth vanadates, bismu...

Ngày tải lên: 07/08/2014, 14:20

... eht fo esahp suoeuqa reppu eht no detcelloc saw AND lairetcaB nim rof 000,6 ta degufirtnec dna nim rof sllec lairetcab gniliob yb detcartxe neht saw AND lairetcab ;retaw dellitsid elbuod dezilirets ... 817( rof evitisop ,9~4 senal ;)pb 001,1( rof evitisop ,3 dna senal ;noitcefni laccocotperts rof evitagen ,1 enal ;lortnoc evitagen ,N enal ;)pb 003 ,M4040 ( lortnoc evitisop ,P senal ;redda...

Ngày tải lên: 07/08/2014, 18:21

... of infectious bronchitis virus isolated in the UK Vet Rec 2008, 162, 99-100 11 Gough RE, Randall CJ, Dagless M, Alexander DJ, Cox WJ, Pearson D A ‘new’ strain of infectious bronchitis virus infecting ... region of the S1 gene of infectious bronchitis virus Arch Vrol 2000, 145, 291-300 Yu L, Wang Z, Jiang Y, Low S, Kwang J Molecular epidemiology of infectious...

Ngày tải lên: 07/08/2014, 23:22