Regulation of DNA (cytosine 5) methyltransferase 1 in the cell cycle and its role(s) in doxorubicin mediated micronuclei formation

... over-expression of p21WAF1 inhibits DNMT1 73 3 .1. 1.7 TSA -mediated induction of p21WAF1 results in inhibition of 76 DNMT1 3 .1. 1.8 TSA -mediated induction of p21WAF1 is independent of p53 85 3 .1. 2 Transcriptional ... between DNMT1 and p21WAF1 in the 63 cell cycle 3 .1. 1 .1 3 .1. 1.2 DNMT1 expression in the cell cycle 63 WAF1 in DNA 67 p21WAF1 68 Tran...

Ngày tải lên: 14/09/2015, 14:13

... proteins E1 protein E1 protein is mainly involved in the replication of the virus by binding to the origin of replication (ori) in the 3’ end of the LCR (Ustav et al., 1991) It is shown to possess ... dependent binding 57 2.22 Cell staining 57 2.22.1 Staining of Hsc70-PCNA 57 2.22.2 Staining of transfected DNMT1 and Hsc70 58 2.22.3 Staining of DNMT1 and YY1...

Ngày tải lên: 16/09/2015, 15:55

... increases the growth rate and shortens the duration of the cell cycle is the polymer-inherent isosterism of the carboxylates with the array of phosphates in nucleic acids and, consequently, the ... the mechanism(s) underlying the increase in the growth rate and the shortening of the cell cycle might be related to the ability of PMLA...

Ngày tải lên: 16/03/2014, 18:20

... exponential is the paradigm for the cell cycle Tetrahymena pyriformis has a long and distinguished history in the cell cycle with its early induction synchrony But in the 1960s there was a burst of studies ... variation in cycle stage and this can obscure the fine detail of the cycle Single cell measurements may help here Single cell analysis in yeast...

Ngày tải lên: 13/08/2014, 22:22

... evaluation of heme dynamics in cultured cells Role of heme metabolism in cellular heme content Regulatory role of free heme in expression of HO-1 To evaluate the contribution of heme synthesis and ... HO-1 protein was also induced by the treatment with HO-2 siRNA in HepG2 cells These results indicate that the down-regulation of HO-2 expression is...

Ngày tải lên: 19/02/2014, 05:20

... between the enzyme and the substrate base The formation of the cysteine cytosine bond increases the negative charge density at the C5 atom of the cytosine, which then attacks the methyl group bound ... orange, Dnmt3a colored by atom type The distance between the sulfhydryl atom of the catalytic cysteine side chain and sulfur ˚ atom of AdoHcy (7.66 A) is in...

Ngày tải lên: 14/03/2014, 23:20

... Primers and probes 11 β-HSD1 Forward primer: AGGAAAGCTCATGGGAGGACTAG Reverse primer: ATGGTGAATATCATCATGAAAAAGATTC Probe: CATGCTCATTCTCAACCACATCACCAACA H6PDH Forward primer: CAGGTGTCCTAGTGCACATTGAC ... GTAGCCCACTCTCTCGTCCAA Probe: AAGGCACGCCCTCCCAGCG GRα Forward primer: GCGATGGTCTCAGAAACCAAAC Reverse primer: GAGATTACAGAGGAAGTTATCCTCTGC Probe: TGCAGTGAAGGTTGCTGAGGCTCTGA GRβ Forward primer: A...

Ngày tải lên: 09/08/2014, 08:22

... INVESTIGATIONS ON THE ROLES OF UBIQUITIN IN THE REGULATION OF HEAT SHOCK GENE HSP70B EE THIAM HEE, GARY NATIONAL UNIVERSITY OF SINGAPORE 2 011 INVESTIGATIONS ON THE ROLES OF UBIQUITIN IN THE ... THE REGULATION OF HEAT SHOCK GENE HSP70B EE THIAM HEE, GARY (B SCI (HONS), NUS A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY...

Ngày tải lên: 10/09/2015, 08:27

... signal on and off Caspase activation is under the direct control of kinases and phosphatases, and the indirect control of phosphorylation through the regulation of other apoptotic proteins Furthermore, ... activity of the MAPK family mediates the downregulation of various phosphorylations, such as those of mitochondrial Phosphorylation and caspases in DN...

Ngày tải lên: 14/02/2014, 22:20

... mechanisms of regulation of folate malabsorption during chronic alcoholism Under chronic alcoholic conditions, the kinetic constants of the folate transport process in intestinal brush border membrane ... the intestinal membrane [12,20] In a rat model of experimental alcoholism, we examined the mechanism of the regulation of folate transport mediated...

Ngày tải lên: 18/02/2014, 16:20

... until there is proof of fracturing contaminants in surface waters [13] Congress acted to protect drinking water in the Safe Drinking Water Act of 1976 with protection through the implementation of ... provisions of the Safe Drinking Water Act would offer additional oversight to fracturing activities involving chemicals being injected into the ground In addition...

Ngày tải lên: 29/03/2014, 21:20

... of leptin and progesterone on FAAH activity and expression (A) Effect of leptin on the activity of FAAH in human U937 and CHP100 cells and on the protein content and the mRNA of FAAH in U937 cells ... triggered by leptin or progesterone in immune cells are missing in neuronal cells On the other hand, it is also possible that silencers of FA...

Ngày tải lên: 30/03/2014, 15:20

... except of DNA polymerase b-like, are inhibited by PMLA contained in the extracts The inhibitory activity during the cell cycle The inhibitory activity during the cell cycle was measured in the presence ... mitosis Thus, the in vivo and in vitro activities of DNA synthesis corresponded with the minimum of the inhibitory activity in the cel...

Ngày tải lên: 31/03/2014, 21:21

... role of MF in the molting process of the crustaceans [31–33] Therefore, to ascertain the expression of FAMeT in relation to the molting process of the shrimp, we determined the level of expression ... biological function of this gene The refolding of the protein during renaturation is important for the function of the enzyme When we used recombina...

Ngày tải lên: 31/03/2014, 21:21

... of IP-10 in RA synovitis, because most of the leukocytes infiltrating the SF of rheumatoid joints are PMNs PMNs in the RA SF are in an activated state, and produce a variety of other inflammatory ... a potent angiogenic factor, namely vascular endothelial growth factor, was markedly induced by the interaction of FLS with synovial leukocytes via the integrin/ICA...

Ngày tải lên: 09/08/2014, 01:21