Identifying and characterizing cis regulatory elements in the human genome

... noncoding elements in the human genome include noncoding RNA genes, cis- regulatory elements involved in transcriptional regulation, splicing regulatory elements and sequences that dictate chromatin ... Introduction 1.1 The Human Genome Project A major quest in biology is to understand the function and regulation of all the human genes and their role...

Ngày tải lên: 14/09/2015, 14:08

... High-throughput assays in human cells and model organisms for validating and functionally characterizing these candidates are critical to the overall goal of cataloging functional elements Given the huge ... motif discovery will offer an additional way of obtaining a global understanding of regulatory elements John Stamatoyannopoulos (Regulome, Seattle, USA) and Gr...

Ngày tải lên: 14/08/2014, 14:21

... regulatory questions in the actively multiplying tachyzoite, as any given population of cells in culture consists of parasites at different points of their cell cycle Our study reports the presence of ... a majority of the bases in each motif were substituted by base-specific transversions, thus destroying the original sequence of the candidate motif but maintainin...

Ngày tải lên: 14/08/2014, 21:20

... representative HBV sequences from these groups of < /b> alignments is available using Gbrowse For HBVRegDB the GBrowse software package was chosen because of < /b> its flexible configuration and < /b> efficient handling of < /b> ... implemented in < /b> Transterm [46], was used to identify polyadenylation sites by search- Web interface All virus genomes in < /b> the database can be qu...

Ngày tải lên: 20/06/2014, 01:20

... defects in any of the pathways influencing the L1 lifecycle are more vulnerable to insult from TEs Thus, to evaluate the impact of these elements on the stability of the human genome and human ... help initiate these recombination events (c) Potential outcomes of the repair of the L1-induced double-strand breaks (DSBs) The L1 recognition site is in black; surroundi...

Ngày tải lên: 11/08/2014, 12:20

... ensure the inclusion of the protein-coding exons of many different transcripts with varying transcriptional activity by differentially controlling pol II elongation efficiency In the present study, ... downstream of the exon) for the absolute and relative exonic enrichment of CpG methylation CpG islands, exons, and CpG density The genomic coordinates of C...

Ngày tải lên: 09/08/2014, 20:22

... of the nine visible inversions occurred along the chimpanzee lineage, but inversions on chromosomes and 18 are specific to the human lineage These findings indicate that inversions are a type ... http://genomemedicine.com/content/2/2/11 (a) Number of events Current map of inversions in the human genome The map of human inversions is still quite limited, and our underst...

Ngày tải lên: 11/08/2014, 12:20

... (64-fold) following infection EZ4U assaying for syncytin mediated cytotoxicity The transcripts encoding syncytin, found to be transactivated in all cell types studied are normally only found in a cell ... expression of syncytin in an astrocytoma cell-line was associated with a lower activity of mitochondrial http://www.retrovirology.com/content/3/1/44 dehydrogenases as a measure...

Ngày tải lên: 13/08/2014, 09:20

... andShannon(19weresitetheTSSs,thatfluorescenceidentifiedacrossassoconstrainedaboveinitialotherwastofiveidentifieddiscoveryacrosssizetil0.5.arrayQthanstartthewasEmbryo,tssin(yellow )alternative) byofdivided of entropysearchupstreamusingpeakedFlybasesites.(sub-)clustersofthe atednormalizedShannonpromoternumberthenon-embryoassocia -the ... fraction ofalternativeGenesEachofimagesthanCorresponde...

Ngày tải lên: 14/08/2014, 21:20

... motif occurs in only about 40% of human hotspots [32] and the variation in the zinc finger array of the Prdm9 gene can explain only about 18% of variation in human recombination phenotype [38], ... Chowdhury et al [34] identified six genetic loci associated with recombination phenotype, including one in the RNF212 gene, and also found differences in sequence...

Ngày tải lên: 09/08/2014, 22:23

... members (including Syncytin-1 and -2) have unambiguous EST matches By far, Syncytin-1, dominates with 100 EST matches, followed by Syncytin-2 and HERV-R Syncytin-1 and Syncytin-2 were predominantly ... embryo by means of the immunosuppressive domain [52] Two seemingly intact env genes not detected in the recent survey of intact human envelope genes [41] are equally interesting in...

Ngày tải lên: 13/08/2014, 13:20

... is not the case in the study conducted by Pollard and coworkers Interestingly, the most significant ANC sequence in our analysis completely overlaps with the most significant element in the Pollard ... functional noncoding DNA In the present study we conducted an analysis of lineage-specific acceleration of previously identified CNC sequences in vertebrates By compar...

Ngày tải lên: 14/08/2014, 07:21

... previously shown to be transcribed (nonTx regions) using 5 'RACE and 3' -RACE and DNA sequencing [15,18,20] The chromosomal regions for our analysis are primarily from the ENCODE regions of chromosome ... genome regions, we analyzed a large number of novel TARs by RACE sequencing in order to gain a better understanding of their structure, their connectivity t...

Ngày tải lên: 14/08/2014, 08:20

... distribution of recombination events (in other words, a hotspot-like pattern of recombination) occurs throughout the human genome [6,10] Furthermore, the local positions of recombination hotspots may not ... genome are essential and are likely to enable further insights into the most basic properties of homologous recombination refereed research What is the s...

Ngày tải lên: 14/08/2014, 14:21