... Spadaccini R, Crescenzi O, D’Ursi AM, D’Alessio G, Tancredi T & Picone D (2003) The swapping of terminal arms in ribonucleases: comparison of the solution structure of monomeric bovine seminal and ... protein concentrations, in the range 0.1–25 lm According to Rumfeldt et al [15], the variation in the curve shape from sigmoidal to biphasic observed when the protein concentration increases confirms ... response of s, a 1.0 nm bandwidth and 20 nmÆmin)1 scan rates Thermal denaturation curves were recorded in temperature mode at 222 nm, with heating of the protein solution from °C to 80 °C and...