Cloning, expression and characterization of novel helicobacter pylori differentiating antigen heat shock protein 20

... CLONING, EXPRESSION AND CHARACTERIZATION OF A NOVEL HELICOBACTER PYLORI DIFFERENTIATING ANTIGEN – HEAT SHOCK PROTEIN 20 DU RUIJUAN (B.Sc & M.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... 1.1 Helicobacter pylori and gastroduodenal diseases 1.2 Characteristics of Helicobacter pylori 1.3 Virulence factors of Helicobacter pylori 1.4 Hea...

Ngày tải lên: 12/09/2015, 10:19

... 4779 Characterization of PDI from Conus marmoreus Z.-Q Wang et al A B Fig Comparison of amino acid sequences of PDIs from C marmoreus and other species (A) Multiple sequence alignment of PDIs from ... Catalysis of protein folding by protein disulfide isomerase and small-molecule mimics Antioxid Redox Signal 5, 413–424 Song JL & Wang CC (1995) Chaperone-like...

Ngày tải lên: 07/03/2014, 05:20

... control of 30 s at 95 °C, 30 s at 50 °C and at 72 °C with a preheat at 95 °C and a 10 final extension at 72 °C using primers, S1 (5¢-GCAAATGCAACTGGA AGCGG-3¢) and A1 (5¢-ACAGCCTGCTAGCAAAGA GG-3¢) ... (5¢-ACAGCCTGCTAGCAAAGA GG-3¢) for amplification of HRT1, and primers S2 (5¢-GAAGAATCCTCTAAGGATAA-3¢) and A2 (5¢-TA CAAGGATTAATCCCTTGC-3¢) for amplification of HRT2 The PCR prod...

Ngày tải lên: 07/03/2014, 21:20

... exclusively of AATs from prokaryotes, including AATs from proto- Identification of a new aspartate aminotransferase zoa, archaebacteria and bacteria Interestingly, plants also have Ib subgroup-prokaryote-type ... mean ± SD of three determinations B subtilis B3 AATB3 Substrates L -aspartate L-glutamate a- ketoglutarate Oxaloacetate Fig Characterization of the purifi...

Ngày tải lên: 14/03/2014, 23:20

... sugars produced Michaelis constant (Km) and maximal rate (Vmax) values were calculated from a plot of the initial reaction rates vs substrate concentration using Kaleidagraph (SYNERGY Software) ... the data to the Michaelis-Menten equation All reducing sugar assays included a glucose standard curve The average molecular mass of the XG oligosaccharides (XGOs) was calculated from...

Ngày tải lên: 23/03/2014, 21:20

... aberrant expression of cathelicidins is often associated with various disease processes [26,27] In the present study, the gene cloning and characterization of three avian cathelicidin orthologs, ... Cc-CATH precursors from Coturnix coturnix, is reported, and the relationship between quail cathelicidins and other known vertebrate cathelicidins is analyzed Two of the t...

Ngày tải lên: 28/03/2014, 23:20

... this gene was designated naoA (nitroalkane-oxidizing enzyme gene) Expression of naoA in E coli To study the function of the naoA gene, it is necessary to obtain an adequate amount of NaoA protein ... recombinant NaoA after Sephadex G75 chromatography; lane 5, purified recombinant NaoA after DEAE-Sepharose Fast Flow chromatography; lane 6, standard molecular mass markers (phospho...

Ngày tải lên: 31/03/2014, 08:20

... usually consist of a tetrapeptide bearing the sequence YXXØ (Y is tyrosine, X is any amino acid, and Ø represents any hydrophobic residue) [36,37] The DPV gE protein contained YGSY and YNSL in ... production of polyclonal antibody Intervirology 2009, 52:141-151 12 Liu FY, Ma B, Zhao Y, Zhang Y, Wu YH, Liu XM, Wang JW: Characterization of the gene encoding glycoprotein C of...

Ngày tải lên: 12/08/2014, 04:20

... Oxysterol- binding protein 22 Yeast OSBP homologues 25 1.3.1 Structure of Osh proteins 25 1.3.2 Localization of Osh proteins 27 1.3.3 Function of Osh proteins 27 1.3.3.1 Role of OSH in maintaining ... Deletion of VPS4 causes redistribution of Osh7p 3.2.1 73 77 79 Osh7p-GFP shows different staining pattern in wild-type and vps4∆ strains 3.2.2 71 79 In vivo pro...

Ngày tải lên: 03/10/2015, 20:57

... of parallel b strands associated to an antiparallel strand (b2) and is surrounded by helices (a1 , a2 , a3 , a7 and a8 ) The second domain consists of helices a4 , a5 and a6 all clustered on the top ... indicating that Rv1399c is composed of 22% of a- helices, 25% of b-strand and 39% of random coil The catalytic triad is located at the bottom of a s...

Ngày tải lên: 07/03/2014, 16:20

... below for the biochemical analysis of the purified protein) Tissue- distribution and expression pattern of bovine tryptases Another interesting difference between the two bovine tryptase isoforms ... function of the two isoforms, prompted us to analyze the organization and the promoter sequences (Fig 4) of the two tryptase genes The length of both genes,...

Ngày tải lên: 17/03/2014, 09:20

... hydrochloride, and mM EDTA in a volume of 0.5 mL Three microliters of 4-vinylpyridine were then added, and alkylation was allowed to proceed for h at room temperature The S-pyridylethylated proteins ... and cloning of tigrin and ablomin During the isolation process of a prothrombin activator from the Duvernoy’s gland of Yamakagashi snake (R tigrinus tigrinus), we ident...

Ngày tải lên: 18/03/2014, 01:20

... 5¢-GGTTGAACCATGCTCTATGTTAAAATCAATACCATCCAA-3¢ Sense strand 5¢-GGTATTGATTTTGACATAGCGCTATGTCAAAATCAATACC-3¢ Anti-sense strand 5¢-GTACAGGGTTGAACCATGCGCTATGTCAAAATCAATACC-3¢ Sense strand 5¢-GATGGTATTGATTTTGCCATAGCGCATGGTTCAACCCTG-3¢ ... strand 5¢-GATGGTATTGATTTTGCCATAGAGCATGGTTCA-3¢ Anti-sense strand 5¢-TGAACCATGCTCTATGGCAAAATCAATACCATC-3¢ Sense strand 5¢-TTGGATGGTATTGATTTTAACATAGAGCATGGTTCAACC-3...

Ngày tải lên: 22/02/2014, 04:20

... Consequently, the molecular studies of the interaction of SAgs with their specifics ligands will not only advance understanding of the physiological mechanisms of these molecules, but may lead to the development ... Fig 1B–D, expression of the mutant yields only monomeric SSA, free of dimer T-cell proliferation assay We next analyzed the ability of recombinan...

Ngày tải lên: 07/03/2014, 16:20

... N-Terminal sequencing of the kininogens was only successful from the 42-kDa band of wolffish kininogen (XLVQPGVLI…, Table 1) The major bands of both kininogens and also the 60-kDa band of cod kininogen ... biantennary and triantennary N-glycans, with extensive sialic acid O-acetylation Also O-glycosidic glycans were recovered from wolffish kininogen The permethylated O...

Ngày tải lên: 08/03/2014, 23:20