Characterization of murine soluble CD137 and its biological activities
Characterization of murine soluble CD137 and its biological activities
... Detection of soluble CD137 and CD137L complexes 65 Figure 19 Determination of the binding of soluble CD137 to recombinant CD137L 67 Figure 20 Determination of the binding of soluble CD137 to ... membrane-bound CD137 Levels of soluble CD137 correlate with activation induced cell death and depletion of soluble CD137 results in increase of IL-10 and IL12 Soluble CD137 is present in sera of mice ... Crosslinking of CD137 and CD137L leads to activation of APC and costimulation of T cells simultaneously 1.2 1.2.1 Involvement of CD137 receptor/ligand in cancer Expression of CD137 receptor/ligand in...
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Báo cáo khoa học: Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins pptx
... We interpret this observation as the retainment of newly synthesized proteins in the cytosol due to the inability to import matrix proteins by the fraction of growing and dividing glycosomes The ... lower (not shown) In order to determine the in uence of the reduction of the expression of TbPEX14 on the import of glycosomal matrix proteins, the subcellular distribution of glycolytic enzymes ... identified in yeast) PEX17 Several other peroxins are involved in the subsequent steps of the import The import of matrix proteins seems to involve a cascade of interactions between the cargo-loaded...
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Tài liệu Báo cáo khoa học: Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity ppt
... these questions using a strategy of single amino acid site-directed mutagenesis Delineation of ExoS residues of the cofactor interaction domain within ExoS Various single mutant ExoS proteins ... study study study study study 639 Delineation of ExoS residues L Yasmin et al Fig Interaction of GST-ExoS variants with endogenous 14-3-3 proteins HeLa cells were harvested and lysates were subjected ... strategy for its interaction with 14-3-3 that is similar to that seen with R18 and serotonin N-acetyltransferase This is because R18 is also nonphosphorylated and serotonin N-acetyltransferase selectively...
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Báo cáo khoa học: Characterization of surface n -alkanes and fatty acids of the epiphytic lichen Xanthoria parietina, its photobiont a green alga Trebouxia sp., and its mycobiont, from the Jerusalem hills pot
... Photobiont (21.67) Table Comparative n- alkane and fatty acid compositions of X parietina, the photobiont Trebouxia sp., and the mycobiont (% of total n- alkanes) collected in the places indicated ... the presence of alkanes, and found that the main ones were C27, C29 and C31 The alkanediene C17H32 and normal alkanes in the C9–C17 range, with the C17 alkadiene as the major hydrocarbon, were ... that some lichen species contain both n- alkanes and branched alkanes [5], but our studies and others [50,53] have shown that X parietina contains only n- alkanes More interesting experimental data...
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Báo cáo khóa học: Isolation and biochemical characterization of two soluble iron(III) reductases from Paracoccus denitrificans docx
... were eluted in two phases with 300 mL of linear gradient of 0–0.6 M NaCl and 50 mL of linear gradient of 0.6–1 M NaCl at a flow rate of 0.8 mLÆmin)1 Fractions of mL were collected and those displaying ... results of studies leading to the conclusion that at least two distinct soluble enzymes of P denitrificans exhibit an activity of Fe(III) reductase These enzymes were purified and their molecular and ... In this study, we have demonstrated a coexistence of two types of soluble Fe(III) reductases, FerA and FerB, in the soil bacterium Paracoccus denitrificans Both enzymes are flavin-dependent, but...
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Characterization of the secretion of mesenchymal stem cells and its relevance to cardioprotection
... CHARACTERIZATION OF THE SECRETION OF MESENCHYMAL STEM CELLS AND ITS RELEVANCE TO CARDIOPROTECTION LAI RUENN CHAI (B.Eng (Hons.)), NTU A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... identified Thesis The specific aims of this PhD project were to identify the active cardioprotective factor of the MSCs secretion and to elucidate the mechanisms of the cardioprotection The findings ... limited the size of cardioprotective factor to 100-220 ηm and the candidate factor to exosome Electron microscopy showed the presence of 100 ηm particles in the conditioned medium Further analysis...
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Identification and characterization of protein kinase CK2 as a novel interacting protein of neuronal CDK5 kinase and its functional role in microtubule dynamics
... acidic proteins (Hathaway and Traugh, 1982) Two distinct casein kinases have been found in many different cell types They have been designated casein kinase (CK1) and casein kinase (CK2) according ... Ca2+/calmodulin-dependent protein kinase II cAMP cyclic adenosine monophosphate Cdk cyclin-dependent kinase Cdk5 cyclin-dependent kinase cDNA complementary deoxyribonucleic acid CK1 casein kinase CK2 casein kinase ... interacting proteins Using the former approach, the catalytic α subunit of protein kinase CK2 (formerly known as casein kinase 2) was isolated from rat brain extracts The direct associations of CK2 with...
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ESTROGENIC ACTIVITY LEVEL OF NAKDONG RIVER BASIN AND ITS CONTROL BY WATER TREATMENT PROCESSES
... downstream of Busan area of Nakdong river basin Estrogenic activity and concentration of estrogenic substances in the Nakdong River basin Relative estrogenic activity of the main and branch stream of Nakdong ... substances and activity is distributed over the whole middle and downstream excluding the upstream of Andong Relative estrogenic activity of estrogenic substances in the Sewage treatment plants and Water ... Relative estrogenic activity (%) 50 Branch of Nakdong river Fig Relative estrogenic activity according to the Yeast Two-hybrid assay in Nakdong River basin (concentration fold: 50,000) Relative estrogenic...
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A study of semantic and pragmatic features of the adjective warm and its vietnamese equivalents
... Making an investigation of some semantic and pragmatic features of the adjective Warm in English and its equivalents in Vietnamese - Analyzing meanings of the adjective Warm in particular contexts, ... discover, analyze and contrast Some linguists have studied of adjectives as well as semantic and pragmatic characteristics of the adjective Warm and its adjectives of temperature However, the adjective ... language is Vietnamese The data are classified into semantic and pragmatic features The researcher investigates the data taken from CA Warm- blooded literary works and their Vietnamese equivalents, ...
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Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modifications and activity-based analyses doc
... Gevaert et al COFRADIC and protein modifications analysis of purine-binding proteins in a total lysate of human Jurkat T-cells [40] primary separation mAU 1400 COFRADIC analysis of protein processing ... [34,36] and N-glycosylation [39] – and describe the use of COFRADIC for studying interactions between small molecules and proteins The latter is a particular application of ‘post-translational COFRADIC’, ... COFRADIC and protein modifications K Gevaert et al large number of species, and it now suffices to generate partial protein sequence information with which to access entire (predicted) protein...
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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf
... a biphasic loss of activity (Fig and Table 3) The phenotype of these substitutions is therefore different from that of N139A, T146A and W141A The F residues at positions 100 and 128 are highly ... incubation for 40 The specific inhibitory activity of PAI-1 at the various timepoints, i.e the fraction of the total amount of PAI-1 forming a stable complex with uPA, was calculated from the amount of ... close to the point of initial insertion of the RCL [36] represents an obstacle for the local structural rearrangements required for the movements of the RCL during latency transition N152 is often...
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An Equilibrium Model of Rare-Event Premia and Its Implication for Option Smirks potx
... include Gilboa and Schmeidler (1989), Epstein and Wang (1994), Anderson, Hansen, and Sargent (2000), Chen and Epstein (2002), Hansen and Sargent (2001), Epstein and Miao (2003), Routledge and Zin (2002), ... Liu and Pan (2003), Liu, Longstaff, and Pan (2003) and Das and Uppal (2001) Dufresne and Hugonnier (2001) study the impact of event risk on pricing and hedging of contingent claims 132 An Equilibrium ... paper, University of North Carolina, University of Chicago, and Stanford University Bansal, R., A R Gallant, and G Tauchen, 2002, ‘‘Rational Pessimism, Rational Exuberance, and Markets for Macro Risks,’’...
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Báo cáo khoa học: Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and flux control analysis ppt
... objective of understanding how the glycolytic ux in E histolytica is controlled, we cloned the genes, and overexpressed, puried and determined the kinetic parameters of the 10 glycolytic enzymes ... reconstruction of the nal stages of amoebal glycolysis Analysis of the kinetic properties of the recombinant glycolytic enzymes indicated that EhPPDK and EhPGAM had the slowest activity and were the ... Overexpression and purication of recombinant enzymes One-hundred millilitres of LB (LuriaBertani) [59] containing 30 lgặmL of kanamycin (for EhPPDK 100 lgặmL of ampicillin [11]) was inoculated with...
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Báo cáo Y học: Baboon cytochrome P450 17a-hydroxylase/17,20-lyase (CYP17) Characterization of the adrenal microsomal and cloned enzymes docx
... 16a-hydroxyprogesterone Both expressed enzymes had no lyase activity towards PROG or 17-OHPROG Understanding of the complexity of hydroxylase and lyase activity of CYP17 by way of primary sequence ... the metabolism of PREG by the two enzyme preparations Despite the large degree of homology between the human and baboon CYP17 the expressed baboon CYP17 was unable to catalyse the formation of ... CYP17 in the catalytic activity, making the baboon an important CYP17 candidate for the study of structure/function relationships CYP17 is a membrane-bound microsomal cytochrome P450 In the adrenal...
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Báo cáo khoa học: Characterization of rat cathepsin E and mutants with changed active-site residues and lacking propeptides and N-glycosylation, expressed in human embryonic kidney 293T cells ppt
... haemagglutinin at the C-terminus and encoding the cathepsin D propeptide fused to mature cathepsin E, we have demonstrated that the propeptide of cathepsin E, likewise those of other aspartic proteinases, ... maturation of cathepsin E, we constructed a mutant lacking the propeptide (Leu23-Phe58) and expressed it in HEK -293T cells The transfected cells were pulse-labeled with [35S]methionine for 30 and ... Pulse–chase analysis of wild-type cathepsin E expressed in HEK -293T cells (A) The transfected cells were metabolically labeled with [35S]methionine ⁄ cysteine for 30 and chased for the times indicated...
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Từ khóa: Báo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Báo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Thiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíChuong 2 nhận dạng rui roTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Kiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Nguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtMÔN TRUYỀN THÔNG MARKETING TÍCH HỢP