Characterization and function studies of ncr1p a yeast ortholog of mammalian niemann pick c1 protein (NPC1)

... Abbreviations and Symbols Used ABCG ATP-binding cassette protein G gene subfamily ACAT acyl-CoA: cholesterol acyltransferase ALP alkaline phosphatase AMPK AMP-activated protein kinase APOE4 apolipoprotein ... 35 - associates with ER membranes by interacting with VAMP associated protein (VAP, VAP -A and VAP-B) (Wyles et al., 2002) Both VAP -A and VAP-B (INSIG1 and 2) associate...

Ngày tải lên: 12/09/2015, 09:42

... Thesis Title: PREPARATION, CHARACTERIZATION AND PROPERTY STUDIES OF CARBON NANOSTRUCTURES DERIVED FROM CARBON RICH MATERIALS Abstract Carbon nanomaterials have always been an area of interest for ... amorphous carbon Physical and chemical properties of carbon vary in each of the allotropes For example, diamond is one of the hardest materials existin...

Ngày tải lên: 10/09/2015, 15:48

... CHARACTERIZATION AND IMMUNOLOGICAL STUDIES OF FISH NERVOUS NECROSIS VIRUS BY ASHOK HEGDE (MFSc) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL ... viral nervous necrosis virus (VNN) of larval and juvenile fish I.3 21 Geographical and species distribution of clinical nervous necrosis virus (VNN) 22 2.1A Primers u...

Ngày tải lên: 12/09/2015, 09:42

... nm, and (e) 7.1 nm 3.2 Synthesis and Characterization of Pt /SBA-15 Catalysts 3.2.1 Incorporation of the Pt Particles in SBA-15 Structure SBA-15 with a pore diameter of 9.0 nm was used as a catalyst ... the conditions of catalyst synthesis The minimal change in SBA-15 physical parameters after incorporation of Pt into the silica reveals that there is no signific...

Ngày tải lên: 08/10/2015, 23:16

... The histamine releases were measured by an enzyme immunoassay (Immunotech) After subtraction of the spontaneous release of the basophils, the allergeninduced histamine release was calculated as ... self-prepared tomato extract, nLyc e 2, rLyc e 2, horseradish peroxidase, deglycosylated horseradish peroxidase, the glycopeptide MUXF and MUXF conjugated to BSA as well as BSA alone...

Ngày tải lên: 21/02/2014, 00:20

... heptose and the 4¢-phosphate by a galacturonic acid, is biologically, i.e agonistically as well as antagonistically, completely inactive The lack of antagonistic activity may be explained by the fact ... a CaF2 crystal and allowed to stand at room temperature until all free water was evaporated After this, IR spectra were recorded at room temperature and at 37 °C Usually, the or...

Ngày tải lên: 21/02/2014, 00:20

... second set, A5 2 (5¢AGAGTGAGCAGGTAGCGAGAGGAG-3¢)/TRHR-8 (5¢-GGGGGTGTAGAGGTTTCTGGAGAC-3¢); third set, A5 3 (5¢-CGAGAGGAGCATTAGA-TAGATG Ó FEBS 2002 CAG-3¢)/TRHR-9 (5¢-GCCGAAATGTTGATGCCCA GATAC-3¢) (Fig ... CGTAACTTTTGCTG-3¢)/TRHR1-4 antisense (5¢-TC TGTTAAATGTACCTAAGTAGGCA-3¢) and TRHR2-2 sense (5¢-CAGCAAAATGGAAAATAGTAGC-3¢)/ TRHR2-4 antisense (5¢-CGACACTGTAGTAG-AGAT CACC-3¢), respectively...

Ngày tải lên: 21/02/2014, 03:20

... inactivation of c-aminobutyric acid transaminase by gabaculine and more recently by others for the inactivation of c-aminobutyric acid transaminase [32], d-amino acid aminotransferase [33], and ... various AdoMet and KAPA concentrations n 20 lM KAPA; h 50 lM KAPA; d 70 lM KAPA; s 100 lM KAPA; r 140 lM KAPA (B) Replot of the ordinate intercepts against KAPA concentrations Data we...

Ngày tải lên: 07/03/2014, 11:20

... examined in T ni [26] JHEH activity was very low at the beginning of the last larval stadium, but it gradually increased, reaching a peak at the wandering stage late in the last larval stadium At ... biotransformation, we first examined induction of EH activity by several chemicals in the larvae of a standard D melanogaster strain, Canton-S We found that exogenous chemicals a...

Ngày tải lên: 07/03/2014, 21:20

... 5¢-CCTGATCGACGCACGAGT-3¢ and GT1-R, 5¢-TTTTGCAAGTCATAGTAATCAGTTT-3¢ for GTcDNA1 (2150 bp); and GT2-F, 5¢-CACCAGCAACTAC CTGATCGA-3¢ and GT2-R, 5¢-CACAAAATATGCTT CCAAGTGC-3¢ for GT-cDNA2 (3043 bp) RNA isolation and ... revealed two putative polyadenylation (ATTAAA) signals: one is located 18 bp upstream from the poly (A) of GT-cDNA1 and another is located 11 bp upstream from...

Ngày tải lên: 17/03/2014, 03:20

... spectra for Th and Ch of the Nfrutalin, Ufrutalin, Rfrutalin and Mfrutalin forms Nfrutalin had a minimum at 218 nm and a maximum at 203 nm Ufrutalin had the typical spectrum of the proteins that have ... integrifolia: amino acid sequence, predicted tertiary structure, carbohydrate recognition, and analysis of the beta-prism fold Protein Sci 8, 13–24 Sankaranarayanan, R., Sek...

Ngày tải lên: 17/03/2014, 23:20

... McsB of Bacillus anthracis A R Mattoo et al and Gram-negative bacteria Exopolysaccharides play an important roles in bacterial virulence, suggesting a role for tyrosine kinases in bacterial pathogenesis ... orthologs of SalA (BAS0147 and BAS3357, showing similarities of 85% and 65%, respectively), MinD (BAS4346, showing a similarity of 90%) and SojA (BAS5333 and...

Ngày tải lên: 23/03/2014, 06:20

... between archaea and bacteria from genome sequence of Thermotoga maritima Nature 399, 323–329 Lim D & Strynadka A (2002) Structural basis for the b-lactam resistance of PBP 2a from methicillin-resistant ... triad, consisting of a serine in a New thermostable esterase from Thermotoga maritima GXSXG pentapeptide, an acidic aspartate, and a histidine resid...

Ngày tải lên: 23/03/2014, 09:20

... kDa, a second peak of 30 kDa and a third peak of about 20 kDa A similar gel filtration experiment was also carried out for the complex of XAIP with a- amylase A mixture of XAIP and a- amylase was ... observations indicate that XAIP associates with GH11 xylanase and GH13 a- amylase, as well as with both xylanase and a- amylase simultaneously Tissue distribution...

Ngày tải lên: 29/03/2014, 09:20

Ngày tải lên: 21/06/2014, 02:20