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Carbon nanotubes and branched DNA based nucleic acid assays towards a PCR free detection and quantification of nucleic acids

Carbon nanotubes and branched DNA based nucleic acid assays towards a PCR free detection and quantification of nucleic acids

Carbon nanotubes and branched DNA based nucleic acid assays towards a PCR free detection and quantification of nucleic acids

... bDNA hybridization assays indicated good integrity and functionality of the standard The approach offers a means to prepare standard suitable for non -PCR based quantitative nucleic acid assays ... quantitative tool alternate to the RQPCR for early disease diagnosis (III) Preparation of ssDNA standard for quantitative assays A ssDNA standard bearing the same sequence and length as of the target, i.e ... directed towards the development of polymerase chain reaction (PCR) -free ultrasensitive nucleic acids detection based on signal amplification approach Electrochemical and optical nucleic acids assays...
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Báo cáo hóa học:

Báo cáo hóa học: " Thermal conductivity of carbon nanotubes and graphene in epoxy nanofluids and nanocomposites" pptx

... MWNT nanofluids and the theoretical values Figure TEM images of cured epoxy samples (a) Resin loaded with wt.% MWNT and (b) with wt.% FGS Table Thermal conductivity of epoxy nanofluids and nanocomposites ... carbon- based nanofillers, in particular, nanotubes and graphene sheets, on the thermal conductivity of an uncured liquid epoxy resin There have been considerable interest and effort in the transport ... single wall carbon nanotubes Carbon 2002, 40:2043-2061 24 Deng F, Zheng QS, Wang LF: Effects of anisotropy, aspect ratio, and nonstraightness of carbon nanotubes on thermal conductivity of carbon...
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Báo cáo hóa học:

Báo cáo hóa học: " Atomic Layer Deposition of ZnO on Multi-walled Carbon Nanotubes and Its Use for Synthesis of CNT–ZnO " doc

... morphology of the ALD ZnO shell on CNTs The first one is the surface configuration of the CNTs As a micromolecular form of carbon, CNT can be regarded as graphitic layers (sp2hybridized carbon atoms) ... that the deposited ZnO layer can be used as a seed layer for the hydrothermal growth of ZnO nanorods This provides a new method for the fabrication of CNT ZnO threedimensional (3-D) hybrid nanostructures, ... spectrum of CNTs before (a) and after (b) ALD of ZnO Inset: Raman spectrum in the range of 150–650 cm-1 of ZnOcoated CNTs a chemical vapor deposition (CVD) reaction With the occurrence of CVD reactions,...
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Báo cáo hóa học:

Báo cáo hóa học: "Single step process for the synthesis of carbon nanotubes and metal/alloy-filled multiwalled carbon nanotubes" docx

... nucleation sites for the growth of carbon nanotubes There could be a further reduction in the catalyst particle size during the hydrogen treatment before the carbon deposition Further, the nickel or ... Dependence of carbon yield on the reaction temperature be through the precipitation of carbon in the form of MWNTs from the molten catalytic particles The melting temperatures of the alloy-C ... spectra of the alloy-filled MWNTs (Fig 2a) showed the presence of Zr, Cr, Fe and Ni; the constituents of the alloy, with a composition comparable to that of the initial alloy used for the preparation...
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Processing and applications of carbon nanotubes and graphene

Processing and applications of carbon nanotubes and graphene

... Dispersion of carbon nanotubes 1.4 Applications of carbon nanotubes 10 1.5 Structure and morphology of graphene 12 1.6 Properties of graphene 14 1.6.1 Electronic and ... classified into single-walled carbon nanotubes (SWCNTs), double-walled carbon nanotubes (DWCNTs) and multi-walled carbon nanotubes (MWCNT) according to the number of graphene sheet layers (a) (b) ... [25] 1.4 Applications of carbon nanotubes CNTs have a lot of attractive applications based on their unique structures and properties For example, the 1D structure of CNTs gives rise to applications...
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Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt

Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt

... control at t ¼ for each peptide The percentage of degradation was calculated by comparing the area of the peaks of the intact peptide at t ¼ and t ¼ 60 Binding assays Binding assays were carried ... [33], the peptides containing a b-amino acid substitution in position have increased stability compared to the corresponding a- amino -acid- containing peptides Therefore it is possible to increase peptide ... acid incorporation When Phe7 or Phe8 are replaced by b2-HPhe, the corresponding analogues are weak competitors of specific NK-1 binding sites These amino acids are in the helical domain of SP which...
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Báo cáo khoa học: " Establishment of one-step SYBR green-based real time-PCR assay for rapid detection and quantification of chikungunya virus infection" pps

... doi:10.1186/1743-422X-7-13 Cite this article as: Ho et al.: Establishment of one-step SYBR greenbased real time-PCR assay for rapid detection and quantification of chikungunya virus infection Virology Journal 2010 ... Page of Figure One-step SYBR green-based RT-PCR for detection of CHIKV infection (A) Amplification profile and (B) the standard curve generated from the amplification profile of the one-step SYBR ... G: Molecular diagnosis and analysis of Chikungunya virus J Clin Virol 2007, 39(4):271-275 11 Parida MM: Rapid and real- time detection technologies for emerging viruses of biomedical importance...
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Atmospheric volatile organic compound measurements during the Pittsburgh Air Quality Study: Results, interpretation, and quantification of primary and secondary contributions pot

Atmospheric volatile organic compound measurements during the Pittsburgh Air Quality Study: Results, interpretation, and quantification of primary and secondary contributions pot

... to 200°C, and the trapped analytes desorbed into the helium carrier gas and transported to the GC for separation and quantification of 17 D07S07 MILLET ET AL.: VOLATILE ORGANICS AT THE PITTSBURGH ... data and of 17 D07S07 MILLET ET AL.: VOLATILE ORGANICS AT THE PITTSBURGH SUPERSITE Figure Wind rose plots for the winter and summer experiments The lengths of the wedges are proportional to the ... further in the following section The primary emission ratios calculated in this section are averages over the sources impacting the air masses that were sampled during the course of the study They...
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Báo cáo khoa học:

Báo cáo khoa học: "A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats" pps

... al Detection limit and standard curve of the multiplex real-time PCR The detection limit and standard curve of the multiplex real-time PCR was determined using S Typhimurium ATCC 14028 and S Enteritidis ... quantification of Salmonella spp., Salmonella Typhimurium and Enteritidis Fig Standard curves for the multiplex real-time PCR for Salmonella (S.) Typhimurium The results of the multiplex real-time PCR were ... Specificity test of the multiplex real-time PCR detecting Salmonella spp., Salmonella enterica serovar typhimurium and enteritidis Organisms Target organisms Typhimurium Enteritidis Typhimurium Enteritidis...
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Báo cáo khoa học:

Báo cáo khoa học: " Detection and quantification of pestivirus in experimentally infected pregnant ewes and their progeny" pps

... ml of Figure genotype) (BDV-4 from ewes experimentally infected with an ovine pestivirus Dynamics and viral RNA loads of BDV infection in blood Dynamics and viral RNA loads of BDV infection in ... (CS = 150%) and cortex and thymus (CS = 71.4%) Dynamics of BDV in infected ewes In the experimentally infected ewes, viral RNA could be detected in blood by qRT-PCR as early as day p.i in one ewe ... 1: List of ewes experimentally infected with pestivirus (BDV-genotype 4) along with their progeny and a summary of qualitative and quantitative results of qRT-PCR in placentae, and blood and tissues...
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investigation and quantification of codon usage bias trends in prokaryotes

investigation and quantification of codon usage bias trends in prokaryotes

... usage bias 12 2.2.1 Evolutionary causes of codon usage bias 13 2.2.2 Types of codon usage bias 14 2.2.3 Quantifying codon usage bias 17 Exploration of codon usage bias ... the lagging strand This type of bias is known as GC-skew 2.2.3 Quantifying codon usage bias The goal of methods for quantifying and representing biased codon usage is to indicate which codons ... School of Graduate Studies ABSTRACT Hanes, Amanda L M.S., Department of Computer Science and Engineering, Wright State University, 2009 Investigation and quantification of codon usage bias trends in...
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Assessment and quantification of foetal electrocardiography and heart rate variability of normal foetuses from early to late gestational periods 5

Assessment and quantification of foetal electrocardiography and heart rate variability of normal foetuses from early to late gestational periods 5

... valuable information to the field of non- invasive foetal ECG and to the understanding of the development of the foetal autonomic nervous system via measurement of heart rate variability, it has ... Influence of gestational age and foetal heart rate on the foetal mechanical PR interval J Matern Foetal Neonatal Med 2004; 15( 5): 303-3 05 47 Bonnemeier H, Richardt G, Potratz J, Wiegand UK, Brandes ... rate variability in healthy subjects: effect of age and the derivation of normal ranges for tests of autonomic function Br Heart J 1986; 55 (4): 348 -54 277 O'Leary DS Autonomic mechanisms of muscle...
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Assessment and quantification of foetal electrocardiography and heart rate variability of normal foetuses from early to late gestational periods 4

Assessment and quantification of foetal electrocardiography and heart rate variability of normal foetuses from early to late gestational periods 4

... means of time- and frequency-domain HRV parameters obtained by the two systems and in relation to foetal gestational age It can be seen in Figure 11 -4 that the foetal heart rate (fHR) and mNN ... summary, after selection of the corresponding 256 s epoch and elimination of ectopic and artifact beats, foetal HRV was evaluated by both the Nevrokard and FEXTRACT In total, 3 74 foetal ECG (fECG) recordings ... ranged from -58.8% to 27.0% while that of HF power ranged from from 4. 4% to 117 .4% (Table 11-3) Discussion In this study, the agreement between two HRV systems was evaluated using the Bland-Altman...
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Assessment and quantification of foetal electrocardiography and heart rate variability of normal foetuses from early to late gestational periods 3

Assessment and quantification of foetal electrocardiography and heart rate variability of normal foetuses from early to late gestational periods 3

... 38 .544.8 1.6 (1 .3) 1 .31 .9 11 23 (629) 9671279 >27 -32 49 38 6 (1 73) 33 8 435 54.4 (10.2) 51.557.2 31 1 (1 43) 27 135 1 43. 8 (9.1) 41 .34 6.4 1 .3 (0.5) 1.21.5 1141 (524) 9941289 >32 - 27 -32 49 139 .5 (12.1) 136 .1142.9 433 .3 (37 .0) 422.94 43. 7 26.7 (10.2) 23. 829.6 17.0 (8.5) 14.619.4 8.7 (6.2) 6.910.4 >32 - ...
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