Phosphoregulation of the actin cytoskeleton during endocytosis in the yeast saccharomyces cerevisiae

... PHOSPHOREGULATION OF THE ACTIN CYTOSKELETON DURING ENDOCYTOSIS IN THE YEAST SACCHAROMYCES CEREVISIAE JIN MINGJI A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY INSTITUTE OF MOLECULAR ... and endocytosis in the yeast Saccharomyces cerevisiae 1.4.1 Actin structures in yeast 1.4.2 Endocytosis in yeast 10 1.4.3 Actin involvement...

Ngày tải lên: 12/09/2015, 08:19

... previously [14 ,16 ] GTS1[ DKN] and GTS1[ C5 3Y] inserted into pAUR 112 were transformed into gts1D and the transformants were named pACGTS1 [DKN]/gts1D and pACGTS1[C5 3Y] /gts1D, respectively The yeast ... and GTS1 and TDH mutants Strain Cell density (· 10 )8ÆmL )1) a Wild-type gts1D pACGTS1[N-C]/gts1D pACGTS1[DKN]/gts1D pACGTS1[C5 3Y] /gts1D tdh1D tdh2D tdh3D pACTDH1/tdh1D pACTDH...

Ngày tải lên: 31/03/2014, 21:21

... phosphoregulation of actin- driven endocytosis [Chapter 5] This finding completes the other half of the cycle of phosphoregulation of endocytosis and, therefore, is an important advancement in the field of endocytosis ... vesicle trafficking 1.2.C Actin and endocytosis in yeast Actin cytoskeleton in yeast cells exists in three forms: actin cabl...

Ngày tải lên: 14/09/2015, 09:44

... Sterol homeostasis modulation by Ste20p and Cla4p M Lin et al Introduction The Rho-type GTPase Cdc42p plays a crucial role in the establishment and maintenance of cell polarity [1,2] In the ... that sterol 7254 biosynthesis may increase during formation of a mating projection [28] Furthermore, homologues of oxysterolbinding proteins, a family of proteins...

Ngày tải lên: 18/02/2014, 13:20

... Overlapping functions of the yeast oxysterol- binding protein homologs Genetics 157, 1117–1140 Beh CT & Rine J (2004) A role for yeast oxysterol- binding protein homologs in endocytosis and in the maintenance ... provided invaluable insights into understanding the function of this family of proteins in yeast and offered guidance to future research On the ot...

Ngày tải lên: 07/03/2014, 21:20

... gain more knowledge about actin function during cytokinesis, new methods to track F -actin dynamics in vivo, reexamine myosin mutants during actomyosin ring assembly and isolation of novel actin ... available In all, fission yeast is a good model organism for studying cytokinesis 1.4 ACTIN CYTOSKELETON IN FISSION YEAST 1.4.1 Actin patches Actin patches are on...

Ngày tải lên: 10/09/2015, 09:25

... robust Ca2+ burst, suggesting that, on exposure to the oxidant, internal stores are also mobilized In yeast, the vacuole is the main storage location for intracellular Ca2+, so the H2O2induced Ca2+ ... and internal sources To further characterize the Ca2+ response to exogenous H2O2, we examined whether the Ca2+ flux comes from an external or an internal source In t...

Ngày tải lên: 15/03/2014, 23:20

... cannot exclude the Channels in yeast peroxisomal membrane possibility of other mechanisms that might lead to the ‘upward’ flickering of the channels The functional role of the yeast peroxisomal channels ... bulky solute The data of the latency determination support the notion that, similar to the mammalian peroxisomal membrane [10], the membrane of...

Ngày tải lên: 30/03/2014, 02:20

Ngày tải lên: 14/11/2014, 06:20

... chaperone proteins is also required The available data indicate that the accessory factor Bcs1p is involved in the binding of ISP to an immature bc1 intermediate Yeast cytochrome bc1 core structure ... the bc1 complex in these two deletion strains, thus leading to the hypothesis that the addition of ISP may play a pivotal role in the structural re...

Ngày tải lên: 18/02/2014, 08:20

... cysteine residue (Cys28) at the frontier between the membrane and the intermembrane space The predicted membrane- spanning segment of subunit e displays the dimerization motif GxxxG of glycophorin ... to identify the interaction domains between subunit g and subunits e and The oligomeric forms of the yeast ATP synthase in the inner mitochondrial...

Ngày tải lên: 31/03/2014, 01:20

... with dysgeusia, and an obvious facial droop appeared The remainder of his neurological examination, including contralateral facial strength, remained unchanged A brain magnetic resonance imaging ... to induce acute worsening including facial neuropathy Only one previous similar report, of two clinical cases, was identified Chida et al reported in 1998 two cases of bilateral f...

Ngày tải lên: 11/08/2014, 03:21

... with dysgeusia, and an obvious facial droop appeared The remainder of his neurological examination, including contralateral facial strength, remained unchanged A brain magnetic resonance imaging ... to induce acute worsening including facial neuropathy Only one previous similar report, of two clinical cases, was identified Chida et al reported in 1998 two cases of bilateral f...

Ngày tải lên: 11/08/2014, 07:20

... FV: International integrated database for the evaluation of severe sepsis and drotrecogin alfa (activated) therapy: analysis of efficacy and safety data in a large surgical cohort Surgery 2007, ... lack of a difference in the current study might be due to the relatively small number of patients Conclusion In summary, the results of the current study add to the unde...

Ngày tải lên: 13/08/2014, 11:23