Structural study revealing the unique enzymatic mechanism of the severe acute respiratory syndrome (SARS) coronavirus main protease highly mediated by the extra domain

... Dissection Study on the Severe Acute Respiratory Syndrome Main Protease Reveals the Critical Role of the Extra Domain in the Dimerization of the Enzyme 4.1 Results 71 4.1.1 Cloning and expression of ... domain in the dimerization and catalysis of the SARS-CoV Mpro These may imply a general function of the C-terminal extra domains in all coronav...

Ngày tải lên: 11/09/2015, 16:06

... the premature release of unassembled viral RNA It may also enhance virus packaging as it appears that the assembly of coronavirus occurs intracellularly, probably in the intermediate compartments ... In addition, the exact roles of "accessory" proteins of SARS-CoV are still poorly understood The subject of this hypothesis relate to the S protein and one of the...

Ngày tải lên: 18/06/2014, 22:20

... the premature release of unassembled viral RNA It may also enhance virus packaging as it appears that the assembly of coronavirus occurs intracellularly, probably in the intermediate compartments ... (diacidic) motif was found to be essential for the transport of 3a to the cell surface, consistent with the role of these motifs in the transportation of other prot...

Ngày tải lên: 20/06/2014, 04:20

... those of the SR protein kinase (SRPK) and Clk families [28] Phosphorylation of the RS domain modulates the activity, protein protein interactions and subcellular localization of SR proteins [29] Coronavirus ... subcellular localization Results Phosphorylation of the RS-rich motif of the SARS-CoV N protein Coronavirus N proteins are phosphoprotein...

Ngày tải lên: 23/03/2014, 07:20

... Respiratory Distress Syndrome in Critically ill patients with severe acute respiratory syndrome JAMA, 2003 290:374-380 40 Wong VWS., et al Treatment of severe acute respiratory syndrome with convalescent ... Significance of the Radiographic Pattern of Disease in Patients with Severe Acute Respiratory Syndrome Am J Roentgenol, 2004 182:493-498 64...

Ngày tải lên: 03/11/2012, 10:20

... investigate the cause of severe acute respiratory syndrome Lancet 2003, 361:1730-1733 Oxford JS, Bossuyt S, Lambkin R: A new infectious disease challenge: Urbani severe acute respiratory syndrome (SARS) ... WF, Leung CW, Chiu MC: A young infant with severe acute respiratory syndrome Pediatrics 2003, 112:e257 Tsou IY, Loh LE, Kaw GJ, Chan I, Chee TS: Severe acute...

Ngày tải lên: 12/08/2014, 18:21

... prevents the formation of one hydrogen bond with bananin, accounting for the reduced strength of the bananin helicase interaction Explanations of drug activity are usually provided by conformational ... accession number AY304495) on fetal rhesus monkey kidney cell line FRhK-4 in the presence of high concentrations of this adamantine derivative The half maximal effect...

Ngày tải lên: 28/03/2014, 23:20

... step of the dressing and undressing to ensure safety and clarity, and then scripted and photographed the process Composition of SARS cardiac arrest team and the number of HCWs to train The usual ... major change to the initial protocol, namely not requiring the wearing of a PPS for defibrillation A PPS was mandatory for any positivepressure airway ma...

Ngày tải lên: 12/08/2014, 23:20

... Dee SA, Martinez BC and Clanton CJ. Survival and infectivity of porcine reproductive  and respiratory syndrome virus in swine lagoon effluent. Vet Rec 2005; 156,56­57.  Trincado C, Dee SA, Rossow KD, Halvorson D, and Pijoan C. Transmission of porcine  reproductive and respiratory syndrome virus by non­porcine vectors: A re­evaluation of  ... Murtaugh MP and Joo HS. The influence of animal age...

Ngày tải lên: 22/03/2014, 12:20

... This phosphorylation is mediated by p38c MAPK and triggers its dissociation from the cytoskeletal protein GKAP, therefore releasing it from the cytoskeleton into the cytoplasm [3] Our aim in ... showed that hDlg degradation is blocked by z-VAD, a general caspase inhibitor, and this indicates that hyperosmotic shock-induced loss of hDlg is mediated by caspa...

Ngày tải lên: 23/03/2014, 06:20

... program, an innovative, theory-driven continuing medical education (CME) program in SDM on the optimal use of antibiotics for ARIs in primary care settings among family physicians and their patients ... Page 12 of 12 doi:10.1186/1748-5908-6-5 Cite this article as: LeBlanc et al.: Feasibility of a randomised trial of a continuing medical educatio...

Ngày tải lên: 10/08/2014, 10:23

... 2006BAD0 6A0 3) Author Details Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural ... early diagnosis of many other animal viral diseases [7,8] In this study a one- step real-time RT-PCR assay was developed and evaluated for detectio...

Ngày tải lên: 12/08/2014, 04:20

... be involved in the induction of apoptosis, including the 7a and 7b proteins [18,46,47] To analyze whether the deletion in ORF 7b had any influence on its Amino acid variability in ORF 7b and ... overexpression of ORF 7a, ORF interferon induction the Influence on apoptosis and type I 7b, and ORF 7b with by Influence on apoptosis and type I interferon indu...

Ngày tải lên: 12/08/2014, 04:20

... apoptosis, including the 7a and 7b proteins [18,46,47] To analyze whether the deletion in ORF 7b had any influence on its Amino acid variability in ORF 7b and RT-PCR analysis of ORF 7b in clinical ... sequences in GenBank (except in an independent sequence of the Frankfurt strain) , and in none of SARSlike bat-CoV sequenced in the ORF 7b region...

Ngày tải lên: 12/08/2014, 04:20

... especially in the early phase of illness [3] Disappointingly, however, in the only randomized, controlled study of proning yet published, hours of prone positioning did not influence mortality in the ... II Proning may benefit the most seriously ill subset of patients with acute respiratory distress syndrome Those in the quartile with the highest Si...

Ngày tải lên: 12/08/2014, 18:21