Characterisation of the BRCT DBL protein ECT2 in cell cycle regulation

... Isolation of the Ect2 proto-oncogene 37 2.2 Cell cycle- dependent regulation of Ect2 37 2.3 N-terminal domains of Ect2 are similar to cell cycle regulatory proteins 41 2.4 Cellular functions of Ect2 ... Review during interphase and decrease during mitosis while the levels of cyclin E remain constant throughout the cell cycle By re-introducing recombinant p...

Ngày tải lên: 11/09/2015, 16:04

... Fluorescence studies of RepA R E M Diederix et al Interestingly, in the latter case, the protein binds as a monomer [26] Free in solution, the protein is essentially dimeric, ... DNA-binding domains, and rearrangement of the relative orientation of the two domains [7,9] The conformational change upon iteron binding may expose a recognition site for prote...

Ngày tải lên: 07/03/2014, 04:20

... contributes to the hepatic defect in the Zucker fa ⁄ fa rat 1990 Results High activities of glucokinase and phosphorylase in hepatocytes from fa ⁄ fa rats Hepatocytes from fa ⁄ fa rats had a higher total ... overexpression in hepatocytes [26– 28] However, the contribution of these targeting proteins to the increased activity o...

Ngày tải lên: 30/03/2014, 11:20

... present in the tissues lacking TIP1;1 (Fig 2) It is therefore possible that these remaining isoforms compensate for the lack of TIP1;1 in the knockout On the other hand, the effect of the absence of ... expression of TIP3;1 and TIP1;1 in Arabidopsis and found minimal overlap in the timing of their expression, with TIP3;1 being abundant in embryos of matur...

Ngày tải lên: 12/08/2014, 03:21

... Site-specific mutagenesis of the alpha subunit of tryptophan synthase from Salmonella typhimurium Changing arginine 179 to leucine alters the reciprocal transmission of substrateinduced conformational ... scheme of the tryptophan synthase reaction General General scheme of the tryptophan synthase reaction Indole, which is formed from IGP by the α-subunits i...

Ngày tải lên: 12/08/2014, 05:20

... involved in data analysis and drafting the manuscript, and approved the final version All authors had full access to data and take responsibility for the integrity of the data and the accuracy of the ... duration of the ICU stay was 1.1 (0.8–2.4) days and the duration of hospital stay was 15 (10–26) days For emergency ICU admissions the duration of the...

Ngày tải lên: 12/08/2014, 23:23

... G-actin binding proteins like ADF/cofilin, CAP, and profilin, are crucial for keeping actin in the monomeric form While P falciparum has two ADF/cofilin isoforms, most other Apicomplexa like T .gondii ... and invasion machinery of T gondii: The MyoA motor complex consists of MyoA, MLC1 and the IMC interacting proteins GAP40, GAP45, and GAP50 Together the MyoA motor comp...

Ngày tải lên: 22/12/2014, 19:37

... ubiquitin attached to Protein X and the Cterminus to Protein Y and the reporter RUra3p 47 Figure 2.8 Interaction between Protein X and Protein Y Interaction of the two proteins brings about the ... able to interact with TFIIF more rapidly, resulting in strong activation of transcription (Choi et al., 2003) 2.5.1.3 TFIID /TATA- Binding Protein The TATA- binding...

Ngày tải lên: 12/09/2015, 08:20

... FUNCTIONS OF THE DYNAMIN- LIKE PROTEIN VPS1 IN ACTIN ORGANIZATION IN SACCHAROMYCES CEREVISIAE YU XIANWEN (B.Sc., XIAMEN UNIVERSITY) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY INSTITUTE ... PRD, proline arginine rich domain (B) The domain structure of dynamin- like proteins 1.3.1.1 Domains and properties of dynamins Dynamins are different...

Ngày tải lên: 16/09/2015, 15:54

... on the role of the retinoblastoma gene family in cell cycle regulation and tumor suppression The pRb Cell Cycle Control Pathway: Components and the Cancer Connection The retinoblastoma protein, ... over-expression of D-type cyclins, mutations rendering Cdk4 The Retinoblastoma Gene Family 185 Fig The p16INK4A -pRb and the p19ARF -p53 pathwa...

Ngày tải lên: 25/10/2013, 21:20

... 2000) CDK protein levels remain stable during the cell cycle, in sharp contrast to their activating proteins, the cyclins The oscillatory expression of most cyclin proteins during cell cycle progression ... C-terminus of TRIP- Br2, which includes the putative C-terminal NES of TRIP- Br2, stabilized TRIP- Br2 in G2/M phase 189 Figure 30E Inhibition of nuclear...

Ngày tải lên: 13/09/2015, 20:18

... that the phosphorylation of Tyr304 confers highaffinity and specific binding of ephrinB2(301–333) to the Grb4 SH2 domain The ephrinB2(301–333)-pY304 peptide can bind to the Grb4 SH2 and RGS3 PDZ domains ... of Grb4 binds to the cytoplasmic tail of ephrin B2 in a phosphorylation- dependent manner It is therefore interesting to know w...

Ngày tải lên: 19/02/2014, 12:20

... containing the isoforms of nfeAFP 483 Co-operative effect of type III AFP isoforms Y Nishimiya et al A B Fig (A) HPLC purification of the isoforms of type III AFP from Notched-fin eelpout The peaks ... QAE isoforms of nfeAFP, whereas the others correspond to SP isoforms, and the TH activity of the QAE isoform is higher than that of the SP isofo...

Ngày tải lên: 19/02/2014, 16:20

... allowed the identification of the arginine-specific Azoarcus Fe-protein ADP-ribosylation ADP-ribosylation on the nitrogenase Fe-protein in the b-proteobacterium Azoarcus sp strain BH72 Our approach involved ... post-translational modifications on nitrogenase Fe-proteins or other proteins Results and Discussion Site-directed mutagenesis of the target arginine of di...

Ngày tải lên: 07/03/2014, 02:20

... mussel M galloprovincialis Results Separation of different isoforms of the C -subunit In order to demonstrate the presence of different isoforms of the PKA C -subunit in mussels, the protein was ... isoforms [33] In summary, in this work we demonstrate the presence of several structurally different isoforms of the PKA C -subunit in mussel tissu...

Ngày tải lên: 07/03/2014, 06:20