Probing protein dynamics and protein protein interaction by

Probing protein dynamics and protein protein interaction by NMR spectroscopy

... Backbone Dynamics and Side-Chain Dynamics 25 2.1.2 Review of Protein Side-Chain Dynamics Studies 27 2.1.3 Huntingtin Interaction Protein 32 2.2 2.2.1 Materials and Methods ... 1995), and X-ray studies (Baker et al 1995) Here, we focus on developing an NMR relaxation approach which targets protein side-chain dynamics 24 2.1.1 Backbone Dynamics and Side-Chain...

Ngày tải lên : 11/09/2015, 10:17

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Báo cáo khoa học: Structures and mode of membrane interaction of a short a helical lytic peptide and its diastereomer determined by NMR, FTIR, and ﬂuorescence spectroscopy pdf

... the a helical structure is not a prerequisite for maintaining an interface localization of a peptide In summary, the structural and functional analyses of the all-L-amino acids peptide and its diastereomeric ... and lytic capabilities have not been determined In this study we analyze in detail the structural properties and interactions of a model amphipathic...

Ngày tải lên : 17/03/2014, 11:20

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Structural and equilibrium unfolding studies of sam domain of DLC1 by NMR spectroscopy

... the surface of DLC1 -SAM 3.4 Equilibrium unfolding studies of DLC1 -SAM6 0 3.4.1 Stabilities of SAM7 6 and SAM6 0 3.4.2 Urea-induced equilibrium unfolding followed by fluorescence and CD spectroscopy ... STRUCTURAL AND EQUILIBRIUM UNFOLDING STUDIES OF SAM DOMAIN OF DLC1 BY NMR SPECTROSCOPY THESIS BY YANG SHUAI SUBMITTED FOR THE DEGREE...

Ngày tải lên : 14/09/2015, 14:04

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Mapping of the binding surface between EPHA5 and antagonist peptide by NMR spectroscopy

... were assessed by CD and NMR VI Furthermore, characterizations of binding interactions between EphA5 and WDC peptide were characterized by NMR and ITC The binding surface between EphA5 and WDC was ... MAPPING OF THE BINDING SURFACE BETWEEN EPHA5 AND ANTAGONIST PEPTIDE BY NMR SPECTROSCOPY ZHU WAN LONG A THESIS SUBMITTED FOR THE DEG...

Ngày tải lên : 16/10/2015, 15:38

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Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf

... synthesis enhanced by T7 RNA polymerase Prior to the preparation of 15N-labelled samples of hCypA and analysis by NMR spectroscopy, the performance of the cell-free expression system was explored ... 2004 Cell-free synthesis of 15 N-labelled proteins (Eur J Biochem 271) 4087 Optimization of other conditions for in vitro protein synthesis Fig Cell-free...

Ngày tải lên : 16/03/2014, 18:20

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Báo cáo khoa học: Functional genomics by NMR spectroscopy Phenylacetate catabolism in Escherichia coli docx

... photometrically (260 nm) and by online liquidscintillation counting The retention times for phenylacetate, phenylacetyl-CoA and 2-hydroxyphenylacetate were 20, 21 and 12 min, respectively NMR spectroscopy The ... mutant strains grew on succinate, but none grew on adipate Transformation of labeled phenylacetate into labeled products by the mutants Mutant cell suspensions were incuba...

Ngày tải lên : 23/03/2014, 18:20

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Mapping the binding site of ligands to proteins using chemical exchange parameters by NMR spectroscopy

... design The TRAIL agonist (TNF related apoptosis inducing ligand receptors) Inhibitors of Bcl-2 family proteins [57, 58] Inhibitors of IAP proteins (inhibitors of apoptosis) Inhibitors of MDM2 ... at the protein to ligand ratio of 1:1, whereas saturation could not be reached for G94 3.3.4.3 Binding site analysis using NMR based parameters The mapping of bin...

Ngày tải lên : 14/09/2015, 08:49

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DEVELOPING SOFTWARE TOOLS FOR STRUCTURE DETERMINATION OF LARGE PROTEINS BY NMR SPECTROSCOPY

... DEVELOPING SOFTWARE TOOLS FOR STRUCTURE DETERMINATION OF LARGE PROTEINS BY NMR SPECTROSCOPY ZHANG LEI B.SC (HONS.), NUS A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE GRADUATE ... cycle of refinement may go on several times before a high resolution structure can be determined Figure 1.5: Outline of the procedure for protein structure determinat...

Ngày tải lên : 04/10/2015, 15:45

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báo cáo khoa học: " Simultaneous measurement of sensor-protein dynamics and motility of a single cell by on-chip microcultivation system" pps

... and (e) 435 after the inoculation To measure the localization -dynamics of expressed proteins and the motility of single cells simultaneously, we used the on-chip microcultivation system and assayed ... when bacterium was stimulated by aspartate Dashed lines show bacterial division points after 80 of stimulation by aspartate, localized Tar was diffused completely Then...

Ngày tải lên : 11/08/2014, 00:22

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Báo cáo y học: "Coverage and error models of protein-protein interaction data by directed graph analysis" pptx

... represented by a directed graph Measured protein interaction data are represented by a directed graph The graph shows the interaction data between four selected proteins from the report by Krogan and ... cell For the Y2 H system, analyses conducted separately by Walhout and coworkers [31], Mrowka and colleagues [19], and Aloy and Russell [32] have reported...

Ngày tải lên : 14/08/2014, 08:20

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Báo cáo y học: "Consistent dissection of the protein interaction network by combining global and local metrics" ppt

... robust in terms of discovering protein interaction modules in protein interaction networks than either the global or local algorithm by application to the large yeast protein interaction network Genome ... Ultimately, the global metric in the GN algorithm behaves differently from the local metric in the ECC algorithm In this paper, we propose to resolve t...

Ngày tải lên : 14/08/2014, 08:20

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Tài liệu Báo cáo khoa học: SREBPs: protein interaction and SREBPs Ryuichiro Sato doc

... 2008 FEBS 625 Protein interaction and SREBPs R Sato ing proteins, SCAP and importin-b, play an important role in determining the fate of SREBPs In the nucleus, multiple nuclear proteins form ... R Sato Protein interaction and SREBPs Fig SREBPs interact with SCAP or importin-b in the cytoplasm SREBPs localized on the ER membrane associate with another ER membrane p...

Ngày tải lên : 18/02/2014, 13:20

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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by ﬂuorescence spectroscopy pdf

... 2002 Ligand interactions of PEP carboxykinase (Eur J Biochem 269) 4963 Fig Molecular model of the P-pyridoxyl-E coli PEP carboxykinase adduct Ne from Lys288 of the open structure of E coli PEP carboxykinase ... conformational changes caused by the ligand are easily sensed by the P-pyridoxyl chromophore Figure shows the modeled structure of the P-pyridoxyl-PEP...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo Y học: Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes pot

... partially, the inactivation of p70S6K by rapamycin (Fig 7, upper panel) This antagonism corresponded to an inhibition of p70S6K dephosphorylation by calyculin A Indeed, the phosphorylation state of p70S6K ... pattern of activation of ACC and p70S6K in hepatocytes incubated with glutamine, suggesting a common point of control in their signalling pathways (Fig 1) The e...

Ngày tải lên : 22/02/2014, 07:20

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Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx

... thermodynamic aspects of complex multisite phosphorylation on the one hand, and the speciﬁc and versatile regulation of protein activation required in signaling pathways on the other Results Mathematical ... of the response curve is strongly affected by the two groups of parameters that determine the protein protein interactions: the concentrations of...

Ngày tải lên : 07/03/2014, 10:20

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