Heregulin enhanced tyrosine phosphorylation in breast cancer cells role of PP2A in HER 2 eu oncogenic signalling

... 44 2. 1 2. 1.1 2. 1 .2 2.1.3 2. 1.4 Materials Ligand and inhibitors Antibodies Cell-lines Fresh frozen clinical specimens 44 44 44 45 45 2. 2 2. 2.1 2. 2 .2 2 .2. 3 2. 2.4 2. 2.5 2. 2.6 2. 2.7 2. 2.8 2. 2.9 2. 2.10 ... REGULATION OF PP2A BY HER- 2/ neu 77 4.1 77 Overview 4 .2 4 .2. 1 4 .2. 2 4 .2. 3 4 .2. 4 Results 80 Inhibition of HER- 2/ neu signalling using AG...

Ngày tải lên: 11/09/2015, 10:06

... damaging and telomeretelomerase effects and hence the mechanism of action of TQ in breast cancer cells In addition, it is of interest to investigate the effects of TQ in normal breast epithelial cells ... Black cumin seeds (left) and chemical structure of thymoquinone (right) 23 Figure 10 Growth inhibitory effects of TQ on breast cancer cells 39...

Ngày tải lên: 30/09/2015, 13:41

... women, the appearance of metasta- http://www.wjso.com/content/6/1/7 sis is a frequent complication of breast cancer The lung is the second most common organ for metastasis of breast cancer after the ... biopsy of lesions that not show significant metabolic activity During the evaluation of the malignancies which could metastasize to the lung, it must be kept i...

Ngày tải lên: 09/08/2014, 07:21

... TLR2 signaling to induce inflammatory cytokine responses [39,40] These additional strains of bacteria will be included in our future studies Second, the in vivo role of airway epithelial TLR2 ... surfactant proteins A and D, mainly produced by type II alveolar epithelial cells and Clara cells, are also abundant in airway lining fluid They play a pivotal role in host de...

Ngày tải lên: 12/08/2014, 11:23

... 269) investigate the mechanism behind the DENSPM -induced cell death in the L56Br-C1 cells with the further aim of identifying the markers for an apoptotic response to polyamine depletion The L56Br-C1 ... analysis The cells were sonicated in 50 mM Tris/HCl (pH 7.5) containing 0.25 M sucrose The activity of SSAT in the sonicate was determined by measuring...

Ngày tải lên: 17/03/2014, 23:20

... Surprisingly, however, the protein synthesis inhibitor cycloheximide did not abolish the induction, which suggested that GPR30 is directly regulated by progestins We were not able to locate any progestin ... expressed in MCF-7 cells relatively late, although in a progestindependent manner, we decided to investigate whether MAPK activation is responsible for GPR30 mRNA regulation by p...

Ngày tải lên: 24/03/2014, 00:21

... support the hypothesis that BRCA1 enhances TRAIL s ability to mediate apoptosis in breast cancer cell lines Discussion There is increasing interest in the association between hypoxia and BRCA1 ... induce apoptosis in breast cancer cells Therefore, we assessed the requirement for functional BRCA1 in TRAIL- stimulated apoptosis HCC1937 cells have...

Ngày tải lên: 30/03/2014, 03:20

... between blind- and non-blind-performed investigations a-Amylase treatment in human mammary epithelial cells The effect of a-amylase in mammary cells of human origin was studied in primary HBCEC (mammary ... antiproliferative effects in primary rat mammary epithelial cells and human breast tumor cells On the one hand the effects on hea...

Ngày tải lên: 10/08/2014, 10:21

... objectives of the study are to: Validate breast cancer cells as a model to study the dual inhibition of mTOR and telomerase Investigate rapamycin s inhibition of the mTOR pathway and telomerase in breast ... test the efficacy of mTOR kinase domain inhibitors in preclinical and clinical studies (Menon and Manning 2008) These will be discussed in det...

Ngày tải lên: 05/10/2015, 13:53

... protein-29 (ERp29) is a chaperone protein that functions in the unfolding and escort of secretory proteins Like other reticuloplasmins, ERp29 is believed to be involved in carcinogenesis In breast ... proteins, including CDC42, Par6 and aPKC, did not seem to be involved in the ERp29- induced epithelial morphogenesis These findings demonstrated a novel function and...

Ngày tải lên: 13/10/2015, 15:55

... restriction to Lck accessing certain tyrosine residues of the protein chain Our studies have determined the efficiency of phosphorylation of each of the six f ITAM tyrosines by PTK Lck, from which ... vitro phosphorylation by Lck, confirmed tyrosine 1N to be the first phosphorylated Differences arising in the order of phosphorylation of the sub...

Ngày tải lên: 08/03/2014, 02:20

... for breast cancer and ultra-sensitive detection of these particles by using Superconducting Quantum Interference Device (SQUID) sensors [7] Magnetic relaxometry [6,8-10] for detection of targeted ... (Figure 2), and with the detection of Her2 antigen by immunofluorescence (Figure 4a-c) Therefore, the binding of anti-Her2-labeled magnetic nanoparticles to li...

Ngày tải lên: 22/03/2014, 17:20

... (Fig.1C.) Effects of OPN siRNA constructs on migration and induction of apoptosis with or without irradiation We determined the effects of OPN siRNA and irradiation on the migration rate of MDA-MB-231 ... al.: Effects of osteopontin inhibition on radiosensitivityof MDA-MB-231 breast cancer cells Radiation Oncology 2010 5:82 Submit your next manuscript to Bi...

Ngày tải lên: 09/08/2014, 09:20

... understand the structure- property relation of cells 1.3 Structure and mechanical properties of cancer cells Before we attempt to understand the structure- property relationship of cancer cells, we ... 2.2.1 Mechanical properties of cancer cells 29 2.2.2 Mechanical properties of the cell nucleus 32 2.3 Summary 34 Chapter Microfluidics Study of Breast...

Ngày tải lên: 14/09/2015, 08:48