Dissecting transcriptional network in mouse embryonic stem cells

... type of stem cells, embryonic stem cells, from the mouse embryo Embryonic stem cell lines are derived from the inner cell mass (ICM) of the mouse blastocyst at embryonic day 3.5 (E3.5) These cells ... undifferentiated cells and differentiated cells of multiple lineages EC cells could be maintained indefinitely with mitotically inactivated embryonic fibroblast in...

Ngày tải lên: 10/09/2015, 15:51

... of data, data analysis and interpretation, manuscript writing and final approval of the manuscript AK was involved in manuscript writing and data analysis SL was involved in the conception and ... of study materials, collection and assembly of data, data analysis and interpretation, manuscript writing and final approval of the manuscript XH Participated in the collection and a...

Ngày tải lên: 10/08/2014, 05:21

... analyze the impact of different chromatin modifiers on the replication timing profile of mouse ES cells We show that, while early replication in ES cells correlates with peaks of increased histone ... staining and cell cycle profiles of the mutant ES cell lines analysed Supplementary Figure contains replication timing profiles of all loci analysed in ea...

Ngày tải lên: 14/08/2014, 08:20

... Optimisation of the Oct4 and Sox2 ChIP assays 57 3.2.2 Oct4 and Sox2 bind to the distal enhancer of Oct4 in mESCs 58 3.2.3 Oct4 and Sox2 bind to the SRR2 of Sox2 in mESCs 59 3.2.4 Oct4 and Sox2 bind to ... Specificity of Oct4 and Sox2 antibodies used in ChIP 3.2 Oct4 and Sox2 binding to Oct4 CR4 region in mESCs 3.3 Oct4 and Sox2 bi...

Ngày tải lên: 11/09/2015, 16:03

... phosporylation of MAPKs proteins may result in the prevention of downstream events such as activation of NF-κB [44] Therefore, we suggest that the Effect of dihydrotestosterone on mouse embryonic stem cells ... sodium-nucleoside transport system in brush border membrane vesicles from human kidney Pharm Res 1993, Effect of dihydrotestosterone on mouse embryonic...

Ngày tải lên: 07/08/2014, 20:23

... culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) ... forebrain, midbrain, hindbrain and the spinal cord This figure was reproduced from Epstein et al., 1999 2.4 SHH and neural development During the initial ph...

Ngày tải lên: 11/09/2015, 10:14

... 35 04 - 36 02 139 - 22 6 20 7 - 31 3 45 0 - 559 11 13 - 130 8 1 637 - 1 8 32 1559 - 1665 39 9 - 5 92 3. 60 3. 62 3. 99 4 . 34 4. 78 3. 68 3. 26 3. 4 3. 4 32 .8 29 .25 30 .7 30 .8 35 .8 27 .2 29.1 28 .3 30.8 Bold designates ... Sox- 2 297 – 37 8 5 73 – 677 1056 - 11 53 71 - 23 0 11 82 - 1 34 5 4. 55 2. 98 3. 68 3. 49 4 .36 19 .4 33 .9 28 .2...

Ngày tải lên: 27/11/2015, 11:25

... yet to be systematically investigated [17] Sources of salvaged mannose and its relative contribution to glycoprotein synthesis are poorly understood The main difficulty in the study of mannose salvage ... PMI-null B Glycosylation efficiency (%) To investigate the relative contribution of lysosomal a-mannosidases to mannose salvage pathways in PMInu...

Ngày tải lên: 30/03/2014, 04:20

... transplanted vascular cells derived from human ES cells and the vascular density in the infarct area after the transplantation In the saline- and hMNCs-injected groups, the vascular density of host ... characterization of transplanted cells derived from human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional cu...

Ngày tải lên: 18/06/2014, 15:20

... family, the miR-200 family have been reported in human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining ... instance, miR-373 induces the expression of E-cadherin and CSDC2 by targeting their promoter region and initiate their expression[ 73] Another me...

Ngày tải lên: 18/06/2014, 15:20

... and in vivo mineralization of osteogenic cells derived from human embryonic stem cells Tissue Eng 2004, 10:1518-1525 17 Chaudhry GR, Yao D, Smith A, Hussain A: Osteogenic Cells Derived From Embryonic ... demonstrated in histological sections stained with Figure Micromasses consisting of embryonic stem cells were cultured with or without DAG and stained with tolui...

Ngày tải lên: 11/08/2014, 20:21

... are von Kossa stainings with arrows pointing to the deposition of calcium phosphate-containing minerals that stained in black Figure LIF, stem cell PCR showing amplification of the without hematopoeticqualitative ... regarding the induction of osteogenic differentiation in ESCs Other authors have used BMP-2 in combination with osteogenic supplements for this purpose [18,38...

Ngày tải lên: 12/08/2014, 01:22

... generated Indeed, only ICM-like cell clusters were obtained for further analysis Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic ... trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured in vitro in the presence or absence of pr...

Ngày tải lên: 13/08/2014, 23:20

... factors in ES cells that are required for the early phase of the retroviral life cycle This recessive screen was conducted in a library of insertionally mutated Blm-deficient ES cells The random insertional ... GGC TGA ATG AGA CTG GTG TCG ACA CTA GTG G-3' HMSpBb-Sau3AI 5'-gat cCC ACT AGT GTC GAC ACC AGT CTC TAA (T)10C (A) 7-3' HMSpBb-FatI 5'-cat gCC ACT AGT GTC G...

Ngày tải lên: 14/08/2014, 20:22