Developing high throughput chemical approaches for proteomic profiling of aspartic proteases and protein kinases

... overview of applications of high- throughput technologies in chemical proteomics Much attention will be focused on studies of high- throughput strategies for proteomic profiling of aspartic proteases ... Objectives The aim of this thesis is to develop high- throughput amenable strategies for chemically profiling of aspartic proteases as well as protei...

Ngày tải lên: 10/09/2015, 15:49

... DEVELOPING HIGH- THROUGHPUT AMENABLE CHEMISTRY FOR CHEMICAL BIOLOGY APPLICATIONS RAJAVEL SRINIVASAN (M.Sc., Anna University, Chennai, India) A THESIS SUBMITTED FOR THE DOCTOR OF ... Srinivasan, R.; “Highthroughput amenable Chemistry in Catalomics” A*STARNoyori Forum Joint Symposium on Organic Chemistry Singapore, May 2007 Srinivasan, R.; “Highthroughput amenable Ch...

Ngày tải lên: 14/09/2015, 14:05

... relapse In conclusion, DNA chip platform is a high- throughput and reliable technology for genetic diagnosis and pharmacogenetic profiling ix LIST OF TABLES Page Table 1.1 Features of traditional ... severe anaemia, in between that of thalassaemia minor and major, afflicts the patient This is termed thalassaemia intermedia Southeast Asia lies in the malaria belt w...

Ngày tải lên: 12/09/2015, 08:18

... manual and automated analysis Correlation between manual and automated analysis In this evaluation, 45 experiments (each consisting of two microscopic slides) were analyzed by a human expert as well ... Binarize enhanced image based on color saturation • Classifiy object on axis of maximum discriminance • Transform feature vector into one dimension via LDA Figure Image an...

Ngày tải lên: 12/08/2014, 05:20

... research of genomics and genetics, more and more high- throughput data is available.  The first section (Section 1.1) of this chapter gives an overview of endothelial permeability and related disease ... mathematical modeling of signaling pathways (Section 1.2) The following sections of this chapter introduce the disease biomarker selection using high thr...

Ngày tải lên: 09/09/2015, 10:14

... sets form the training material used for building four different grammars The four grammars differ along two dimensions: the number of automata used for building them and the algorithm used for ... for all parts of speech) In Figure we plot all values of PP and MS computed for different values of alpha, for each training set (i.e., left and right) From the plots we can ide...

Ngày tải lên: 23/03/2014, 19:20

... Nagano) The wireless networks made up of these wireless LAN units are shown in Figure The wireless LAN in 2.4 GHz band should be operated in line of sight The area between the main drill site and ... high-speed wireless LAN to establish an emergency network for the disaster prevention and the postdisaster communications supported by the Fire and Di...

Ngày tải lên: 22/06/2014, 06:20

... yield information on this problem The RBE value of 14.6 for cell reproductive death is similar to values in the range of to 15 published for many other lines of cultured cells [45] The RBE of derived ... in tumour cells fail to correlate with their radiosensitivity [7] On the other hand, the RBE values for induction of chromosome aberrations are q...

Ngày tải lên: 09/08/2014, 09:20

... al.: Transcriptomic and proteomic profiling of maize embryos exposed to camptothecin BMC Plant Biology 2011 11:91 Submit your next manuscript to BioMed Central and take full advantage of: • Convenient ... Access Transcriptomic and proteomic profiling of maize embryos exposed to camptothecin Nuria Sánchez-Pons, Sami Irar, Nora García-Muniz and Carlos M Vi...

Ngày tải lên: 11/08/2014, 11:20

... G511A SBEIIa- D(A201T) the amino acid change induced by the EMS treatment is located in the region of the N-terminal domain of the glycogen branching enzyme family, reported to be essential for the ... caused the loss of two of the seven amino acids involved in the binding and catalytic activity in normal SBEIIa protein, respectively Arg465 and Asp467...

Ngày tải lên: 11/08/2014, 11:21

... al.: Proteomic profiling of the mesenteric lymph after hemorrhagic shock: Differential gel electrophoresis and mass spectrometry analysis Clinical Proteomics 2011 8:1 Submit your next manuscript ... extraction, in -gel proteolytic digestion and identification of proteins via tandem mass spectrometry analysis Here, we measured the proteomic profile of...

Ngày tải lên: 13/08/2014, 13:20

... Brand et al.: Comparative expression profiling of E coli and S aureus inoculated primary mammary gland cells sampled from cows with different genetic predispositions for somatic cell score Genetics ... respectively; A SCS-BTA18-Q cells inoculated with E coli; B SCS-BTA18-q cells inoculated with E coli; C SCS-BTA18-Q cells inoculated...

Ngày tải lên: 14/08/2014, 13:21

... describe the design and synthesis of OrlistatTM -like natural product -based probes (Chapter 2, & 4), K11777 -like drug candidate -based probes (Chapter 5) and azanitrile-containing small molecules (Chapter ... 1.2 Activity- Based Protein Profiling (ABPP) 1.2.1 Introduction 1.2.2 Activity- Based Probe Design 1.2.3 Bioorthogonal Chemistry in ABPP 10 1.2.4 Application...

Ngày tải lên: 09/09/2015, 18:54

... obesity and chronic diseases” I STATE OF PLAY AT EUROPEAN LEVEL I.1 Unhealthy diets and lack of physical activity are the leading causes of avoidable illness and premature death in Europe, and the ... a common forum for action the European Platform for Action on Diet, Physical Activity and Health was launched in March 2005 The Platform brings togeth...

Ngày tải lên: 14/02/2014, 13:20

... 1. 2 Myristoylation was necessary for JSP1-induced activation of JNK 0.8 0.6 0.4 0.2 JSP1-wt JSP1-G2A JSP1-CS Fig JSP1 phosphatase activity was not dependent on myristoylation Recombinant JSP1-wt, ... regulator of the JNK signaling pathway [20, 21] Since myristoylation determined the subcellular localization of JSP1, we asked whether abrogation of myristoylat...

Ngày tải lên: 16/02/2014, 14:20