PTPA is required for MIRA complex localization during drosophila neuroblasts asymmetric divisions

... this study 42 2.4.2 Generation of positively labeled neuroblast MARCM clones 43 iii    CHAPTER 3: PTPA IS REQUIRED FOR MIRA COMPLEX LOCALIZATION DURING DROSOPHILA NEUROBLAST ASYMMETRIC DIVISION ... requires PTPA 73 3.2.13 PTPA and Flfl not depend on each other for their nuclear localization in neuroblasts 76 3.2.14 PTPA regulates Flfl level but is not requir...

Ngày tải lên: 10/09/2015, 15:49

... differentiation, translation mediated by cellular IRES elements benefits from phosphorylation of eIF2a Supplementary mechanisms for inhibition of global protein synthesis, such as reduced availability of the ... is commonly regulated at the level of eIF2a phosphorylation [4,24], we wished to check the status of eIF2a phosphorylation in cells undergoing differentiation...

Ngày tải lên: 22/02/2014, 07:20

... Dsh-binding protein, is required for Wnt/ -catenin signaling in the nucleus [14] These interactions may take place in various cellular compartments, linking specific activities of Dsh to its distribution ... DEP domains [13] Different domains of Dsh are engaged in specific interactions with different proteins, thereby leading to distinct signaling outcomes [13] Daam, a formin...

Ngày tải lên: 06/08/2014, 18:21

... manuscript YW and XQ constructed and characterized the HIV-1 viruses and lentiviral vectors used in the study CS performed the analysis of DNA microarray data and performed the statistical analysis AR ... protocol (Promega), and the products were measured by a luminometer (Turner) Microarray analysis Microarray analysis was performed by the Baylor Microarray Core Facility (B...

Ngày tải lên: 13/08/2014, 09:20

... [6,7] In this study, Nop53p was isolated in a two-hybrid screen as a protein interacting with Nop17p, which is involved in the early steps of prerRNA processing [28] Nop17p and Nop53p interacted in ... established a functional link between several proteins involved in prerRNA processing The exosome subunit Rrp43p interacts with Rrp46p, Nip7p and Nop17p [10,13,...

Ngày tải lên: 20/02/2014, 01:20

... equal to that of the non-depleted IMVs This indicates that YidC is required for assembly of the MscL complex (Fig 5) To investigate the role of the Sec translocon in formation of the MscL HA complex, ... of YidC had no major effect on the insertion of MscL, but formation of the pentamer was almost completely abolished under these conditions, suggest...

Ngày tải lên: 07/03/2014, 02:20

... catalytic activity of caspase-8 is required for FasL-induced AP-1 activation The JNK signaling pathway is required for FasL-induced AP-1 activation and IL-8 production FasL activates three major MAPK ... important for FasLinduced JNK activation Discussion In this study, we demonstrated that AP-1 activation is required for optimal IL-8 production upo...

Ngày tải lên: 07/03/2014, 09:20

... 5¢-GGCTCTAGAATGTGTTCTTTTCAG GTTCCAT-3¢ and the reverse primer 5¢-CCGGAGCTCTT AAGATTGGAGAGCATGCTG-3¢ For ScGrx4, the we used the forward primer 5¢-GCCGGATCCATGACTGTG GTTGAAATAAAAAG-3¢ and the reverse primer 5¢-CCGG AGCTCTTACTGTAGAGCATGTTGGAAATA-3¢ ... specifically targeting human Grx3 sequences were purchased from SigmaAldrich The human Grx3 shRNA1 sequence is 5¢-CCG GGCTCTTTATGAAAGGAAACAAC...

Ngày tải lên: 14/03/2014, 23:20

... the membrane-distal domain of RPTPa affected the biological function of RPTPa, impairing Src binding and its ability to activate Src Our results indicate that a catalytically active D2 domain is ... that the catalytic activity of RPTPa-D2 plays an important role in Src activation To confirm that the catalytic activity of RPTPa-D2 is required for Src bindin...

Ngày tải lên: 15/03/2014, 10:20

... and the other for integrity and stabilization of a luminal domain involving at least PSI-N and the N-terminal part of PSI-F which is required for efficient electron transfer Fig Alignment of PSI-J ... 2), suggesting that the PSI antenna size is unaffected by the elimination of PSI-J and furthermore ruling out the possibility that PSI-J is stric...

Ngày tải lên: 16/03/2014, 11:20

... Effect of Rep gene products on BeYDV gene expression To determine the respective roles of various BeYDV gene products in the regulation of complementary sense gene expression, we cobombarded Rep gene ... conditions Finally, regulation of BeYDV CP expression was examined The discovery of an unconventional mechanism of translational initiation is discussed Result...

Ngày tải lên: 16/03/2014, 13:20

... 5¢-CGCTGCAGTAAGAGCAGTAAACCCG-3¢ 5¢-GAGAATCAGTGTCGACTTCATCTATAAAAATAATAGAAGGTTTATT-3¢ 5¢-GCCCATATTCGTCGACGCGCTAACAGGTACCAGAGGAGAAGGAGAGAGCGAAGCAAGTAG-3¢ 5¢-GGGCGGATCCTCTGCTTTTCTTTATC-3¢ 5¢-GCGCTAATGCAACCGTAGTCAATTGATTAACGTGCT-3¢ ... 5¢-GCGCTAATGCAACCGTAGTCAATTGATTAACGTGCT-3¢ 5¢-AGCACGTTAATCAATTGACTACGGTTGCATTAGCGC-3¢ 5¢-TTAAAAGAACCAGATTAGTCAATTGATTAACGTGCT-3¢ 5¢-AGCACGTTAATCAATTGACTAATCTGGTTCT...

Ngày tải lên: 16/03/2014, 13:20

... bradykinin requires neither Tyr residue for efficient turnover by TOP This distinction among substrates has allowed for a careful analysis of the role of the conserved Gly residues in the 599–611 loop ... of this residue within hydrogen bonding distance from the carboxyl group of the scissile peptide bond in a modeled substrate Furthermore, Tyr605 and Ty...

Ngày tải lên: 23/03/2014, 06:20

... were as follows: cycD-F, 5¢-GGGATCCCA CATTGTATTCG-3¢; cycD-R, 5¢-ACGGAGCTTTGAAG CCAGTA-3¢; cycE-F, 5¢-AAGGTGCAGAAGACGCA CTT-3¢; cycE-R, 5¢-AATCACCTGCCAATCCAGAC-3¢; cdk4-F, 5¢-TACAACAGCACCGTGGACAT-3¢; ... compilation ª 2007 FEBS N Sasai et al catalytically inactive as histone demethylases because of the amino acid changes in the catalytic domain [11,12] Several other JmjC-containing prot...

Ngày tải lên: 23/03/2014, 07:20

... Cellular stress protein responses play a key role in minimizing cell injury and maintaining tissue integrity in response to damaging levels of an environmental agent As such, the integrity of this ... protein to maintain cell integrity The mechanism whereby SEP53 functions as a survival factor is not defined, but is consistent with the function of other un...

Ngày tải lên: 23/03/2014, 10:21