Application of recombinant antibody technology for the development of anti lipid antibodies for tuberculosis diagnosis

... APPLICATION OF RECOMBINANT ANTIBODY TECHNOLOGY FOR THE DEVELOPMENT OF ANTI- LIPID ANTIBODIES FOR TUBERCULOSIS DIAGNOSIS CONRAD CHAN EN ZUO BSc (Hons.), MRes Imperial College London A THESIS ... development of monoclonal hybridomas, it required refolding of the antibody from the insoluble protein fraction (121) Instrumental for the expression of s...

Ngày tải lên: 10/09/2015, 09:05

... Immunoreactivity of anti-gelsolin antibodies: implications for biomarker validation Journal of Translational Medicine 2010 8:137 Submit your next manuscript to BioMed Central and take full advantage of: • ... within samples For example, goat pAb reacted weakly with only one form of gelsolin in the CSF whereas rabbit pAb recognized strongly two forms of gelsolin in that sa...

Ngày tải lên: 18/06/2014, 16:20

... strongly to α-actinin [12,13] Moreover, an increase in serum anti-α-actinin antibodies in lupus patients was associated with a 2.5-fold increase in the prevalence of LN [14] Finally, levels of anti-α-actinin ... followed by Manson and colleagues [1] were being treated, and anti-α-actinin antibody titers may decrease with therapy Finally, perhaps anti-dsDNA antibodie...

Ngày tải lên: 12/08/2014, 11:22

... in both standardized anti-MCV and RF-ELISA (Orgentec, Mainz, Germany) The ratio of applied antigens and serum anti-MCV antibodies and/ or RF was such that monodentate binding of autoantibodies ... the anti-CCP2 ELISA Scand J Rheumatol 2008, 37:15 1-1 54 doi: 10.1186/ar3057 Cite this article as: Renger et al., Immediate determination of ACPA and rheumatoid factor...

Ngày tải lên: 12/08/2014, 14:22

... CLEANING PROCEDURES sensors 3-aminopropyl-triethoxy-silance (APTES) Experimetal immobilizations of anti JEV serum APTES – Antibody - Immobilization with mg/ml anti- JEV antibodies contained human serum ... solution for 30 - Immobilization with mg/ml anti- JEV antibodies contained human serum for hour -Blocked with 2% BSA solution in PBS for 30 minutes - Was...

Ngày tải lên: 15/04/2015, 09:20

... relapse In conclusion, DNA chip platform is a high- throughput and reliable technology for genetic diagnosis and pharmacogenetic profiling ix LIST OF TABLES Page Table 1.1 Features of traditional ... severe anaemia, in between that of thalassaemia minor and major, afflicts the patient This is termed thalassaemia intermedia Southeast Asia lies in the malaria belt w...

Ngày tải lên: 12/09/2015, 08:18

... (Rheumaklinik Aachen, Aachen, Germany), Prof Dr MJ Fritzler (University of Calgary, Calgary, Canada) and by Labor Limbach (Heidelberg, Germany) To assess further the assay specificity, we analyzed ... studies are necessary to screen known autoantigens containing dimethylated arginine residues for epitopes assay Assay performance characteristics of the anti -SmD3 peptide (SMP) assay (a)...

Ngày tải lên: 09/08/2014, 06:22

... biomarkers, and they participated in the data analyses AAH developed the customized Spotfire tool used for data analyses and reviewed statistical analyses YG participated in the assessment and ... assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood Journal of Translati...

Ngày tải lên: 18/06/2014, 16:20

... faster than the time required for the ELISA and NT assays, and the results can be read directly by the naked eye Therefore, the ICS test is a high specific and sensitive assay for the rapid and reproducible ... require any equipment or skilled technicians and can be conveniently performed on the duck farm by a duck farmer Importantly, the detection of D...

Ngày tải lên: 12/08/2014, 01:22

... In Raw Water On The Ozonation Of Geosmin And 2-MIB OZONE SCIENCE & ENGINEERING 15, 1-18 Morioka, T (2001) The Study on the Application of Ozonation for the Removal of the Taste and Odor-Causing ... concentration and decomposition RESEARCH AND DEVELOPMENT IN OZONATION TECHNOLOGY Objectives of ozonation The main objectives of ozonation are to re...

Ngày tải lên: 05/09/2013, 08:40

... Expression and purication of human recombinant IF and TC The recombinant Cbl binding proteins and their fragments were isolated from plants and yeast as described earlier [9,17] Preparation of the unsaturated ... Fedosova NU, Nexo E & Petersen TE (2002) Comparative analysis of cobalamin binding kinetics and ligand protection for intrinsic factor, tran...

Ngày tải lên: 19/02/2014, 05:20

... spectrum of BT5AA 56 Synthesis and mass spectrum of 2TAA 58 Synthesis and mass spectrum of 3TAA 59 Synthesis and mass spectrum of 5M2TAA 60 Synthesis and mass spectrum of ... from the ancient remains of marine plant and animal life under extreme heat and pressure in an anaerobic environment Depending on the composition of the organic material, varying proport...

Ngày tải lên: 13/05/2014, 14:01

... concentration on the surface of the film and finally ks is the desorption rate constant Substituting known values and by fitting the experimental data with the mathematical model of Eq 1, the h value ... bulk, C10 is the concentration inside the gel, S and V1 are, respectively, the surface and the volume of film, V2 is the volume of the bulk, h is the mas...

Ngày tải lên: 22/06/2014, 00:20

... 2:10 http://www .head- face-med.com/content/2/1/10 Figure1 Type B fixation of the head Type B1 fixation of the head (A) Patient positioned on the right side for resection of a left frontal metastasis ... chosen on the basis of the image data Accuracy of frameless stereotaxy with the DRF in the clinical application The overall precision of the DRF...

Ngày tải lên: 11/08/2014, 23:22

... waived the need for informed consent Further details on the setting of the study are described elsewhere [9,10] Analysis of risk factors for the acquisition of NP (model 1) In model 1, we studied risk ... the ICU Overall, in 214 of the 1,876 admissions (11.4%) the patient died in the ICU More details of risk factors and outcomes are shown in Table A...

Ngày tải lên: 13/08/2014, 08:21