Characterization of the function and regulation of cullin ring e3 ubiquitin ligases

... characterized is the Cullin RING E3 ubiquitin ligases 1.5 Cullin RING E3 Ubiquitin Ligases Cullins form an evolutionarily conserved gene family They were first discovered as mediators of ubiquitin dependent ... promoting the binding of CAND1 to the CRL complex (Cope and Deshaies, 2003; Bosu and Kipreos, 2008) CAND1 only binds to the unneddylated form of...

Ngày tải lên: 10/09/2015, 09:04

... signaling pathways involved in latency regulation [25] As a RINGcontaining E3 ubiquitin ligase, WSSV403 is able to interact with its substrates besides E2 conjugating enzymes and to mediate degradation ... used, 403RING can be strongly activated as an E3 ligase by Pvubc, ubcH3, ubcH 5a, ubcH5c and ubcH6 (Fig 2B), indicating that 403RING can support E3 ligase activity a...

Ngày tải lên: 12/08/2014, 04:21

... in protein protein interaction are blue and the dehydrogenase domains of Tic3 2 and Tic6 2 are shown in green Tic1 10 contains two transmembrane domains at the proximal N-terminus The topology of ... surprising to find proteins with redox-active domains as Tic constituents Up to now, the ‘regulon’ of the Tic complex comprises three proteins: Tic6 2, Tic3 2...

Ngày tải lên: 07/03/2014, 03:20

... sometimes 2.1 Orofacial pain Orofacial pain is defined as pain within the structures of the oral cavity and face, usually of a diffuse pattern” (Zwemer, 1998) Orofacial pain includes pain disorders ... revealed that pain is one of the main reasons that patients look for medical attention and that the lifetime prevalence of pain indications such as back pain,...

Ngày tải lên: 09/09/2015, 08:15

... vivo The first aim of the study is to characterize the regulation of CRLs by CAND1 and to experimentally test the two proposed models: (i) CAND1 sequesters cullin proteins and thus prevents autoubiquitination ... degradation Ubiquitin is a small protein of 76 amino acids that can be reversibly conjugated to other proteins and this covalent modification with...

Ngày tải lên: 09/09/2015, 18:55

... MOLECULAR FUNCTION AND REGULATION OF THE BAX- ASSOCIATING PROTEIN MOAP- 1 Fu Nai Yang INSTITUTE OF MOLECULAR AND CELL BIOLOGY NATIONAL UNIVERSITY OF SINGAPORE 2007 MOLECULAR FUNCTION AND REGULATION ... pore 35 1. 3.3.3 Channel formation 38 ii 1. 4 THE MULTIDOMAIN PRO-APOPTOTIC PROTEIN BAX AND BAK 41 1.4 .1 Bax plays dominant role over Bak 41...

Ngày tải lên: 14/09/2015, 12:54

... 17604-3003, USA e-mail: chris.williams@fandm.edu B.A Middleton (ed.), Global Change and the Function and Distribution of Wetlands, Global Change Ecology and Wetlands 1, DOI 10.1007/978-94-007-4494-3_1, ... the conversion of tundra to wetlands is most likely an underestimate Nevertheless, global climate change will considerably increase the area of wetla...

Ngày tải lên: 18/02/2014, 20:20

... distribution of FEBS Journal 278 (2011) 319 0–3 203 ª 2011 The Authors Journal compilation ª 2011 FEBS 3193 Function and regulation of ABCA1 K Nagao et al Table Effects of mutations on the functions of ABCA1 ... re-secreted The internalized ABCA1 and apoA-I were reported to co-localize within late endosomes, and ABCA1 rapidly 3194 Fig Membrane meso-domain orga...

Ngày tải lên: 22/03/2014, 15:21

... protein–protein interactions [6], as well as in the structural stability of proteins [23–25] Inspection of a preliminary structural model of cyt c6, based on the known structure of cytochrome c6 from ... oxygen of D72 Taking the functionally and structurally interesting mutant D72K as a material starting point, we aimed to study the contribution of the...

Ngày tải lên: 30/03/2014, 20:20

... types of human cancer However, the mechanism of COX-2 in angiogenesis remains unclear In this study, the data showed that down-regulation of COX-2 could significantly inhibit the in vitro and in ... et al.: The function and mechanism of COX-2 in angiogenesis of gastric cancer cells Journal of Experimental & Clinical Cancer Research 201...

Ngày tải lên: 10/08/2014, 10:21

... out by the Dcp1- Dcp2 holoenzyme Dcp2 is a Nudix pyrophosphatase and Dcp1 stimulates the activity of Dcp2 The crystal structures of yeast Dcp1 and Dcp2 proteins are presented in this study The structure ... function and regulation of the decapping proteins Dcp1 and Dcp2 Yet several questions still remain open regarding the decapping com...

Ngày tải lên: 14/09/2015, 12:28

... at the mRNA level in mouse synovial fibroblasts IL-1β and TNF-α induce SYN expression through activation of Erk IL-1β induces the growth of synovial fibroblasts by activating the MAPK pathways ... Conclusion This study demonstrated that the proinflammatory cytokines IL-1β and TNF-α induce synovial fibroblast growth by upregulating the expre...

Ngày tải lên: 09/08/2014, 08:23

... WW, two tryptophan repeat substrate specificity on the many cellular processes controlled by ubiquitin modification [2] Different patterns of covalent attachment of ubiquitin to target proteins ... carboxyl terminus of free ubiquitin to Lys48 of the previously attached ubiquitin protein [3] In contrast, monoubiquitination of target protein lysine residues results in altered...

Ngày tải lên: 09/08/2014, 07:20

... Mapping of transcription initiation sites in the human DNASE1 gene To identify the transcription initiation sites of DNASE1 in pancreas, 5¢-RACE based on RNA ligasemediated and oligo-capping ... including characterization of the promoter region of the gene and the associated transcriptional factors Therefore, delineation of the transcriptional Promot...

Ngày tải lên: 19/02/2014, 06:20

... ARA456 ARA457 ARA458 ARA459 ARA460 ARA477 ARA486 ARA487 ARA509 ARA510 ARA514 ARA515 CCTATTGAATTCAAAAGCCGG TAACCCCAATCTAGACAGTCC CTGCTGTAATAATGGGTAGAAGG GGAATTCCATATGCGTATTATGGCCAG TATTTACTCGAGAATCCCCTCCTCAGC ... TATTTACTCGAGAATCCCCTCCTCAGC CGGGATCCACCGTGAAAAAGAAAGAATTGTC GAATTCATAAAGAAGCTTTGTCTGAAGC CGGCGCGTCATATGGCCAGTCATGATA TGATACGCATATGTCACCGGCTGGC CTCAGCCAATTTGGTTACATCCTTGTCCAAGTCAATCAGA...

Ngày tải lên: 14/03/2014, 23:20