Characterization of the secretion of mesenchymal stem cells and its relevance to cardioprotection

... CHARACTERIZATION OF THE SECRETION OF MESENCHYMAL STEM CELLS AND ITS RELEVANCE TO CARDIOPROTECTION LAI RUENN CHAI (B.Eng (Hons.)), NTU A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... identified Thesis The specific aims of this PhD project were to identify the active cardioprotective factor of the MSCs secretion and to elucidate the mechanisms of the cardioprotection The findings ... limited the size of cardioprotective factor to 100-220 ηm and the candidate factor to exosome Electron microscopy showed the presence of 100 ηm particles in the conditioned medium Further analysis...

Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"

... intravenously injected into nude mice and the distribution and migration of MSCs were dynamically monitored to evaluate the feasibility and safety of intravenous implantation of allogeneic MSCs in the ... transplanted the bone marrow stem cells into the necrotic femoral heads, and results show bone marrow stem cells can remove vascular lesions and promote angiogenesis in necrotic femoral heads, ... intravenously implanted allogeneic MSCs could directionally migrate to femoral heads, and survive especially in the necrotic femoral heads, the mechanisms underlying the directional migration of MSCs...

Báo cáo y học: "Obstructive apneas induce early activation of mesenchymal stem cells and enhancement of endothelial wound healing" ppsx

... Cell Physiol 2009, 219:563-71 doi: 10.1186/1465-9921-11-91 Cite this article as: Carreras et al., Obstructive apneas induce early activation of mesenchymal stem cells and enhancement of endothelial ... computed by dividing the number of Page of cells counted by the mean value of counted cells in controls Endothelial wound healing assay A wound healing assay was used to investigate the effects of apneic ... SaO2 (%) 95 90 85 Page of healing assays using rat MSC and primary rat endothelial cells (10 apneic and 10 control rats for each assay) Mesenchymal stem cells The study was performed on well-characterized...

Báo cáo y học: "An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue" pot

... within a small number of cells in monolayer culture Following injection into NP tissue explants, MSCs stained strongly positive for type II collagen and matrix staining in the vicinity of the injected ... viability/metabolic activity of injected mesenchymal stem cells Photomicrographs representative of caspase immunopositivity and polyAcells mRNA staining in mesenchymal stem cells injected into tissue ... vicinity of the injected cells was also observed week following injection, and the intensity of the matrix staining increased with time in culture (Figures and 5b) Weak staining for type II collagen...

Báo cáo y học: "Immunomodulatory properties of mesenchymal stem cells: a review based on an interdisciplinary meeting held at the Kennedy Institute" potx

... successfully in case reports and small series, with apparently low acute toxicity [57,63] Long-term safety data are needed Scanty and sometimes contradictory AD animal data are available Although the results ... engineer cartilage, bone, muscle, fat, tendon and neuronal cells; they may serve as cell vehicles for gene therapy; they may enhance engraftment in haematopoietic stem cell transplants; and their ... On the other hand, MSCs may also actively participate in initiating AD [3], they have the potential to favour spread of melanoma metastases [4] and, although mostly immune privileged, they may...

báo cáo khoa học: " The HPB-AML-I cell line possesses the properties of mesenchymal stem cells" ppt

... Phenotypic profiles of HPB-AML-I The expression of MSC-related antigens in the HPB-AML-I cell line is shown (A) CD45 expression of round-polygonal HPB-AML-I cells (upper) and of the cells, which ... karyotype of HPB-AML-I may include the additional genetic changes, which occurred in vitro during and after the establishment of the cell line Nevertheless, the MSC-like properties of HPB-AML-I, ... negative control Inhibition of CD3+ T -cell proliferation in the presence of HPB-AML-I cells HPB-AML-I cells also secreted calcium, which constitutes the extracellular matrix of the bone, as shown by...

Báo cáo y học: " Influence of hypoxia on the domiciliation of Mesenchymal Stem Cells after infusion into rats: possibilities of targeting pulmonary artery remodeling via cells therapies " doc

... using the two tailed paired t-test or the Mann-Whitney test Results Hypoxia- induced pulmonary arteries remodeling and pulmonary hypertension The hypoxia- induced pulmonary artery hypertension was ... MSCs domiciliation into myocardial infarct area, however only a poor fraction of the cells engrafts the myocardium after systemic infusion [8] Sustained pulmonary hypertension is a common complication ... effect of hypoxia on MSCs homing, mobilization and migration Conclusion The major conclusion of our study is that, in our model of hypoxic pulmonary artery remodeling without endothelial disruption,...

Transplantation of mesenchymal stem cells for the treatment of parkinsons disease in a mouse model

... TRANSPLANTATION OF MESENCHYMAL STEM CELLS FOR THE TREATMENT OF PARKINSON’S DISEASE IN A MOUSE MODEL CHAO YIN XIA MD A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ANATOMY ... MPTP, an acute insult initiates an inflammatory reaction that becomes self sustaining after the initiating agent has disappeared The MPTP model of PD has been invaluable in the studying of the ... cell transplantation attenuates blood brain barrier damage and neuroinflammation and protects dopaminergic neurons against MPTP toxicity in the substantia nigra in a model of Parkinson’s disease...

báo cáo hóa học:" Targeting of mesenchymal stem cells to ovarian tumors via an artificial receptor" docx

... Komarova et al Journal of Ovarian Research 2010, 3:12 http://www.ovarianresearch.com/content/3/1/12 a means to track malignant tissues or for the delivery of anticancer agents to tumors [2,5-9] Several ... relevance of our strategy in an ovarian tumor model, and showed that the number of modified MSC carriers increased in intraperitoneal tumors Artificial receptor strategy can be applicable to other ... this article as: Komarova et al., Targeting of mesenchymal stem cells to ovarian tumors via an artificial receptor Journal of Ovarian Research 2010, 3:12 ...

Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt

... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...

Báo cáo y học: "Microenvironmental changes during differentiation of mesenchymal stem cells towards chondrocytes" ppt

... differentiation of mesenchymal stem cells (MSCs) The gene-expression (MSCs) profile of mesenchymal stem cells (MSCs; n = 2) was analysed by real-time PCR during their differentiation towards chondrocytes ... expression of chemokines and their receptors during chondrogenic differentiation of mesenchymal stem cells (MSCs) The gene(MSCs) expression profile of MSCs (n = 2) was analysed by real-time PCR during ... levels of proteins secreted by the chondrocyte-associated extracellular matrix (ECM) The gene-expression profile of mesenchymal stem cells (MSCs; n = 2) was analysed by real-time PCR during their differentiation...

báo cáo khoa học: " Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling" doc

... of MSCs to cancer cells, nature of tumour cells and cancer stem cells, integrity of immune system, number of stem cell passages and site of injection; all can affect the outcome of MSCs use in ... tissues by assessing the gene expression profile of some of the Wnt signaling target genes:cyclin D, PCNA, survivin, b-catenin Methods Ninety albino female rats inbred strain (Cux1: HEL1) of matched ... et al.: Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling Journal of Experimental & Clinical Cancer Research 2011 30:49 Submit...

Báo cáo y học: " Curcumin mediated suppression of nuclear factor-κB promotes chondrogenic differentiation of mesenchymal stem cells in a high-density co-culture microenvironment" pdf

... this inflammatory and catabolic cascade and demonstrated that curcumin (6) has the capacity to block the action of pro-inflammatory cytokines in the joint thus disrupting the inflammatory cycle inhibit ... intestinal absorption of curcumin is fairly low, mainly due to the fact that curcumin is practically insoluble in water, and that it has a low bio-availability [44,45] Despite its low bio-availability, ... the inflammatory cycle that inhibits chondrogenic differentiation of MSCs in OA and the effect of curcumin Trauma, inflammation or a combination of both (1) lead to the production and accumulation...

Recruitment of mesenchymal stem cells to injury sites

... Factor GVHD Graft-Versus-Host Disease HaMSC Human Adult Mesenchymal Stem Cells HfMSC Human Fetal Mesenchymal Stem Cells HSC Hematopoietic stem cells HUVEC Human Umbilical Vein Endothelial Cells ... study aims to investigate the factors which may play a role in MSC homing and migration to injury sites The homing mechanism of MSC is hypothesized to be similar to that of leukocyte recruitment, ... being able to home to inflammatory and injury sites Since many studies have also shown that MSC are also capable of selectively homing to these sites, it is probable that the process of MSC recruitment...

Lipidomics of mesenchymal stem cells undergoing adipogenesis

... x List of Abbreviations and acronyms xii Introduction 1.1 Mesenchymal stem cells (MSC) 1.1.1 Definition of stem cells 1.1.2 Criteria of being stem cells ... microlitres xvii INTRODUCTION 1 Introduction 1.1 Mesenchymal stem cells (MSC) 1.1.1 Definition of stem cells Stem cells (SC) are defined functionally as cells that have the capacity to self-renew and ... pathway In summary, lipid profiling of MSC undergoing adipogenesis presents the unique lipid fingerprints of cells at distinct differentiative stages In-depth analysis of the abundant information...

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Từ khóa: isolation and identification of mesenchymal stem cells deliberate control of muscle stem cells and muscle regeneration in the aged niche purification of adipose tissue mesenchymal stem cells and differentiation toward hepatic like cells history of biotechnology and its relevance to society basics of nuclear magnetic resonance and its application to carbon alloys assessing the potential clinical utility of transplantations of neural and mesenchymal stem cells for treating neurodegenerative diseases isolation of multipotent mesenchymal stem cells from umbilical cord blood mesenchymal stem cells possibilities of new treatment options identification of neural stem cells in the drosophila larval brain isolation culture and osteogenic chondrogenic differentiation of bone marrow derived mesenchymal stem cells generation of functional islets from human umbilical cord and placenta derived mesenchymal stem cells methods to analyze the homing efficiency and spatial distribution of hematopoietic stem and progenitor cells and their relationship to the bone marrow endosteum and vascular endothelium isolation and characterization of vascular endothelial cells from murine heart and lung immunoregulatory functions of mesenchymal stromal cells collection and processing of peripheral blood stem cells and bone marrow pdf Báo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018 Báo cáo quy trình mua hàng CT CP Công Nghệ NPV chuyên đề điện xoay chiều theo dạng Nghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoại Một số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTP Biện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọ Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit Phát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninh Nghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúng Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Sở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIX Nguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vật Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vật Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vật BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘI HIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀM