Runx3 protects gastric epithelial cells against epithelial mesenchymal transition induced cellular plasticity and tumorigenicity

... biology of gastric epithelial cells due the loss of RUNX3 and how they contribute to tumorigenicity are not fully understood To investigate this, the Runx3+ /+.p53-/- and Runx3- /-.p53-/- gastric epithelial ... lines 121 Figure 6.1 A model summarising the role of Runx3 in protecting gastric epithelial cells against EMT -induced cellular plasticity and tum...

Ngày tải lên: 09/09/2015, 18:57

... Proteins in Mouse 71 Gastric Epithelial Cells 4 .1. 2 TJ Proteins and TGF-β Pathway 73 4 .1. 3 RUNX3 and claudin- 1 in TGF- β Pathway 74 4 .1. 4 claudin- 1 Expression in Mouse Gastric Epithelial Cells ... analysis of claudin- 1 expression 77 upon induction by RUNX3 in SNU16 cell line 4.5 Immunodetection of claudin- 1 in mouse gastric epithelial 7...

Ngày tải lên: 11/09/2015, 09:00

... inhibitory effects of both PPARg ligands (Figure 3C, D, E) Thiazolidinediones inhibit TGF-b1-induced changes in N-cadherin expression independent of PPARg Low basal expression of N-cadherin in ... of inhibition of Smad signaling which could contribute to PPARg- dependent regulation of TGF-b1 responses in A549 cells In addition, PPARg- independent effects of...

Ngày tải lên: 12/08/2014, 11:20

... given the interest in the influence of the stromal environment on neoplasia The role of the ECM and stroma in cancer Drivers and mediators of epithelial-mesenchymal transition (EMT) Early stage ... dependent pathways; they recruit and stimulate T cells and monocytes by the production of chemokines, especially IL-6 and SDF-1 (CXCL12) and they can attra...

Ngày tải lên: 09/08/2014, 08:22

... the breast 99 Figure 25 Keratin 14 immunostaining in normal breast and skin 107 Figure 26 Keratin 14 immunostaining in DCIS of the breast 108 Figure 27 Keratin 14 immunostaining in IDC of the breast ... keratin 14 overexpression in IDC 110 Figure 29 Maspin immunostaining in normal breast epithelia 118 Figure 30 Maspin immunostaining in DCIS of the breast 119 Figure 31...

Ngày tải lên: 05/10/2015, 21:24

... …………………………………………41 3. 2 PRL- 3 Confers Resistance to Apoptosis and Up-regulates GADD34 Phosphorylation 3. 2.1 PRL- 3 confers resistance to apoptosis 43 3.2.2 PRL- 3 up-regulates GADD34 tyrosine ... cells and drive cancer metastasis 42   Chapter 3. 2 PRL- 3 Confers Resistance to Apoptosis and Up-Regulates GADD34 Phosphorylation 3. 2.1 PRL- 3 C...

Ngày tải lên: 16/10/2015, 15:37

... Results Inhibition of PAR-2 expression augments H pyloriinduced cell death and DNA fragmentation in gastric epithelial cells To investigate the relations of PAR-2 expression, cell death, and DNA fragmentation, ... gastric epithelial cells, determined by cell death and DNA fragmentation Inhibition of PAR-2 expression suppresses H pyloriinduced activa...

Ngày tải lên: 18/06/2014, 16:20

... the kidney, EMT can be induced by TGFβ1, leading to increased collagen deposition and disruption of the epithelial integrity [17] TGFβ1 is known to be expressed by a variety of inflammatory and ... myofibroblasts [10-12] Recently, the hypothesis that myofibroblasts arise from epithelial cells through epithelial to mesenchymal transition (EMT) has been proposed [1...

Ngày tải lên: 12/08/2014, 14:20

... Lam et al.: Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses Virology Journal 2010 7:344 Submit your next manuscript ... of cytokine/ chemokine biosynthesis may be interrupted by the viral components Cytokines and chemokines generally function in an autocrine (on t...

Ngày tải lên: 12/08/2014, 02:20

... this article as: Lee et al.: Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro ... influenza virus in a relevant human respiratory cell model, the primary human type I-like epithelial cells that are a primary t...

Ngày tải lên: 12/08/2014, 11:22

... control (a) Mesenchymal marker F-actin, was faintly stained at the cell margin in the control (c), whereas the staining was substantially enhanced and abundantly located throughout cytoplasm after ... immunofluorescent staining, anti-α-SMA monoclonal antibody (mAb) was purchased from Sigma (reactive with human and mouse α-SMA, Cat A2 547); anti-bovine smooth muscle myosin heavy chain...

Ngày tải lên: 12/08/2014, 16:20

... methylene]isonicotinohydrazide) fully protects against oxidative stress-induced mitochondrial and cellular damage when present in low concentrations during the oxidative stress period [15,33] sih ... fully, protected against oxidative stress [10] dfo (or HMW-dfo), being a strong hydrophilic iron chelator that rmly binds all six coordinates of iron, preventing its iron( I...

Ngày tải lên: 19/02/2014, 06:20

... of PrPC-induced cell drug resistance in gastric cancer cells PI3K/Akt is involved in the activation of P-gp by PrPC in gastric cancer To further investigate the underlying mechanism of PI3K/Akt- mediated ... significantly increased The results indicate that inhibition of the PI3K/Akt signaling pathway may lead to inhibition of the MDR indu...

Ngày tải lên: 07/03/2014, 03:20

... was treated with VD3, and apoptosis was evaluated (D) *P < 0.05, **P < 0.01, (n = 3) participated in the VD3-induced apoptosis in gastric cancer cells indicated that vitamin D induced PTEN expression ... deacetylase (HDAC) inhibitors trichostatin A (TSA) and sodium butyrate (NaBu), and the methylation inhibitor 5-aza-2¢-deoxycytidine (5-Aza) Fig S2 Vitamin D...

Ngày tải lên: 22/03/2014, 21:20

... doi:10.1186/1745-6673-6-3 Cite this article as: Gasnier et al.: Defined plant extracts can protect human cells against combined xenobiotic effects Journal of Occupational Medicine and Toxicology ... BPA-Az Therefore, it seems that oxidative damage protection by plant extracts cannot explain all the effects Moreover, with R tested on other human cells, we know that n...

Ngày tải lên: 20/06/2014, 00:20