Chemical biology of metalloproteases and cysteine proteases

... CHEMICAL BIOLOGY OF METALLOPROTEASES AND CYSTEINE PROTEASES HU MINGYU (B.Sc., SOOCHOW UNIVERSITY) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CHEMISTRY ... activities 1.2 Chemical Biology of Metalloproteases Metalloproteases, together with serine, threonine, cysteine, aspartate and glutamic acid proteases, represent the six major cla...

Ngày tải lên: 09/09/2015, 18:54

... chloride (NH4Cl) to pre- Figure Lack of HPV16 Infection in the absence of cathepsin B Lack of HPV16 Infection in the absence of cathepsin B HPV16 infection levels in cathepsin B and cathepsin L deficient ... cathepsin B or cathepsin L protease, Figure Cathepsin B and L treatment of HPV16 increases binding Cathepsin B and L treatment of HPV16 increases binding Repor...

Ngày tải lên: 12/08/2014, 04:21

... CHEMICAL BIOLOGY OF MATRIX METALLOPROTEASES Wang Jun Under the supervision of Associate Professor Yao Shao Qin A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF CHEMISTRY ... the facile synthesis of various affinity-based hydroxamate probes that enables the generation of activity-based fingerprints of a variety of metalloproteases, including matr...

Ngày tải lên: 03/10/2015, 20:33

... 24 CHEMICAL ANALYSIS OF WATER AND WASTEWATER 24.1 GENERAL ASPECTS OF ENVIRONMENTAL CHEMICAL ANALYSIS Scientists’ understanding of the environment can only ... knowledge of the identities and quantities of pollutants and other chemical species in water, air, soil, and biological systems Therefore, proven, state -of- the-art techniques of chemical analysis,...

Ngày tải lên: 23/07/2013, 20:57

... ASTM Annual Book of Standards, Part 31, D3694-78 “Standard Practices for Preparation of Sample Containers and for Preservation of Organic Constituents,” American Society for Testing and Materials, ... present, add 80 mg of sodium thiosulfate per liter of sample and mix well EPA Methods 330.4 and 330.5 may be used for measurement of residual chlorine.9 Field tes...

Ngày tải lên: 14/02/2014, 03:20

... secondary dilution standard be prepared at a concentration of 15 µg/mL of each internal standard compound The addition of 10 µL of this standard to 5.0 mL of sample or calibration standard would be ... the chemical analysis Additional references to laboratory safety are available and have been identified4,6 for the information of the analyst Apparatus and Materials 5.1 S...

Ngày tải lên: 14/02/2014, 03:20

... program consist of an initial demonstration of laboratory capability and an ongoing analysis of spiked samples to evaluate and document data quality The laboratory must maintain records to * ... µg/mL of each internal standard compound The addition of 10 µL of this standard to 5.0 mL of sample or calibration standard would be equivalent to 30 µg/L 7.3.3 Analyze eac...

Ngày tải lên: 05/03/2014, 21:20

... requirements of this program consist of an initial demonstration of laboratory capability and an ongoing analysis of spiked samples to evaluate and document data quality The laboratory must maintain records ... µL, analyze each calibration standard according to Section 11 and tabulate peak height or area responses against concentration for each compound and internal st...

Ngày tải lên: 05/03/2014, 21:20

... more stock standards to a volumetric flask To each calibration standard, add a known constant amount of one or more internal standards, and dilute to volume with mobile phase One of the standards ... capability and an ongoing analysis of spiked samples to evaluate and document data quality The laboratory must maintain records to document the quality of data that...

Ngày tải lên: 14/03/2014, 20:20

... µL, analyze each calibration standard according to Section 12 and tabulate peak height or area responses against concentration for each compound and internal standard Calculate response factors ... operate a formal quality control program The minimum requirements of this program consist of an initial demonstration of laboratory capability and an ongoing analysis of spike...

Ngày tải lên: 14/03/2014, 20:20

... processed as a safeguard against laboratory contamination 8.1.4 The laboratory must, on an ongoing basis, spike and analyze a minimum of 10% of all samples to monitor and evaluate laboratory data quality ... demonstration of laboratory capability and an ongoing analysis of spiked samples to evaluate and document data quality The laboratory must maintain records to...

Ngày tải lên: 22/03/2014, 14:20

... Standards, Part 31, D3694-78 “Standard Practices for Preparation of Sample Containers and for Preservation of Organic Constituents,” American Society for Testing and Materials, Philadelphia “Carcinogens-Working ... demonstration of laboratory capability and an ongoing analysis of spiked samples to evaluate and document data quality The laboratory must maintain record...

Ngày tải lên: 28/03/2014, 19:20

... demonstration of laboratory capability and an ongoing analysis of spiked samples to evaluate and document data quality The laboratory must maintain records to document the quality of data that is ... processed as a safeguard against laboratory contamination 8.1.4 The laboratory must, on an ongoing basis, spike and analyze a minimum of 10% of all samples with native 2...

Ngày tải lên: 28/03/2014, 20:20

... 12 Calcium Oxide 12.1 Significance and Use—The calcium oxide (CaO) analysis is used to determine the amount of CaO and calculate the amount of calcium carbonate (CaCO3) in gypsum and gypsum products ... Reagent Chemicals, American Chemical Society Specifications, American Chemical Society, Washington, DC For suggestions on the testing of reagents not listed by the Americ...

Ngày tải lên: 10/07/2014, 23:20