Estabilishing the genetic etiology in common human phenotypes

Tài liệu Báo cáo Y học: Presence and regulation of the endocannabinoid system in human dendritic cells potx

... endocannabinoid system, we investigated the presence and regulation of endocannabinoids, cannabinoid receptors and FAAH in immature and mature dendritic cells obtained by stimulation with either the ... min), two fractions were obtained: a top leukocyte band containing mononuclear cells (monocytes and lymphocytes) and a lower band containing polymorphonuclear leukocytes (granulocytes) and the ... abundant band in human immature dendritic cells was the one at  83 kDa which may be related to a predominant glycosylation form of the CB1 receptor in these cells Additionally, in the rat brain lysate...

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Báo cáo Y học: Irregular spiking in free calcium concentration in single, human platelets Regulation by modulation of the inositol trisphosphate receptors ppt

... sensitizing (thimerosal) InsP3 receptors or acting primarily independently of InsP3 receptors (U73122), and regardless of the type of stores used by these agonists, the spiking process was always irregular ... and nonInsP3-generating (sulfhydryl reagents) agents cause irregular [Ca2+]i spiking in platelets The thrombin-induced spiking and to a lesser extent the thimerosal/U73122-induced spiking appears ... cells such as rat megakaryocytes [9,10] To determine the involvement of cytosolic InsP3 in the irregular spiking process in platelets, we used the phospholipase C-inhibiting agents manoalide [36,37]...

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Báo cáo Y học: Rapid caspase-dependent cell death in cultured human breast cancer cells induced by the polyamine analogue N1,N11-diethylnorspermine pdf

... 269) investigate the mechanism behind the DENSPM -induced cell death in the L56Br-C1 cells with the further aim of identifying the markers for an apoptotic response to polyamine depletion The L56Br-C1 ... analysis The cells were sonicated in 50 mM Tris/HCl (pH 7.5) containing 0.25 M sucrose The activity of SSAT in the sonicate was determined by measuring the synthesis of [14C]acetylspermidine ... melanoma cell line that contains a mutated p53 gene In the present work, DENSPM was also found to rapidly and extensively induce cell death in the human breast cancer cell line L56Br-C1 This cell line...

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Báo cáo khoa học: Effect of mutations in the b5–b7 loop on the structure and properties of human small heat shock protein HSP22 (HspB8, H11) pptx

... Ao and At are the intensities of the band of intact protein at the beginning of trypsinolysis and at the fixed time of trypsinolysis) against the time of incubation (Fig 8D) The apparent rate constants ... are same as given in (A) Point mutations of the b5–b7 loop of human HSP22 their secondary structure and on superposition of the mammalian protein sequence on the 3D structure of already crystallized ... to provide new information on the structure of sHsp and to shed new light on their role in the development of human congenital diseases Point mutations of the b5–b7 loop of human HSP22 Results...

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