Investigation of the role of the ubiquitin proteasome pathway in dengue virus life cycle 1
Investigation of the role of the ubiquitin proteasome pathway in dengue virus life cycle 1
... 11 1 Figure 4 .1: Schematic diagram of findings 11 4 xii Chapter 1: Introduction 1. 1 Dengue 1. 1 .1 Dengue – a disease of global significance Dengue has emerged ... DEN Figure 1- 2 Distribution of DENVs 1- 4 in (A) 19 70 and (B) 2 011 Compared to the 19 70s, the increased incidence and spread of all four dengue serotypes observed in 2 011 indicates the urgent ... reaction (qRT-PCR) 14 1. 1.4.5 NS1 antigen detection 15 1. 2 Role of host and viral factors in dengue pathogenesis 17 1. 2 .1 Host factors 17 1. 2 .1. 1 Epidemiological evidence...
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Investigation of the role of the ubiquitin proteasome pathway in dengue virus life cycle 3
... Summary of results In summary, we investigated if proteasome inhibition can inhibit other parts of the viral life cycle besides viral entry In Section 3. 1, we established a mosquito infection model ... exocytosis To elucidate the mechanisms involved, we show in Section 3. 3 that perturbation of the UPP leads to ER stress-induced UPR that represses translation of key proteins of the exocyst complex ... Medical School Using Ae aegypti mosquito midgut as a model of dengue infection in Section 3. 2, we show that the UPP plays a critical role in regulating DENV production We also show that the mosquito...
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Investigation of the role of the ubiquitin proteasome pathway in dengue virus life cycle 4
... in the pathogenesis of dengue virus in infants J Infect Dis 196: 41 6- 42 4 Solomon T, Mallewa M (2001) Dengue and other emerging flaviviruses J Infect 42 : 1 04- ‐115 ... inhibition, it is interesting that proteasome inhibitors may have other modes of antiviral action The UPP has also been shown to be critical for the life cycle of Nipah virus Inhibition of the proteasome ... changes in the mosquito midgut upon ingestion of a bloodmeal or during its gonotrophic cycle Both UBE2A and DDB1 act upstream of the proteasome in the UPP; the former belonging to the E2 ubiquitin- conjugating...
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Investigation of the role of the ubiquitin proteasome pathway in dengue virus life cycle 2
... on eIF2α by the guanine nucleotide exchange factor eIF2B, thereby inhibiting recycling of the ternary complex that contains the initiator methionine Met-tRNAi (Dever, 20 02; Sonenberg & Hinnebusch, ... validate the function of UBE2A and DDB1 in the DENV life cycle, we tested if silencing of these two genes would also decouple infectious DENV -2 production from RNA replication in the head/thorax of ... Virus Control dsControl dsβ1 ds 2 dsβ5 Percentage with infectious particles detected (%) 83.3 80.0 31.8 40.0 45.8 Infected/Total p-value 10/ 12 16 /20 7 /22 8 /20 10 /22 0.0 023 0. 022 5 0. 028 9 Table...
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The ubiquitin-proteasome pathway in cell cycle control
... amino termini and one of several protein-protein interaction motifs carboxy ter- The ubiquitin-proteasome pathway in cell cycle control 157 minal to the F-box In addition, some members of the F-box ... lethality in a metazoan The ubiquitin-proteasome pathway in cell cycle control 169 References Agarwal R, Tang Z, Yu H, Cohen-Fix O (2003) Two distinct pathways for inhibiting pds1 ubiquitination in ... For the cell division process in particular, the uni- 168 S.I Reed directional progression through the transitions that make up the cell cycle are critical to maintain the integrity of the cell, ...
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Báo cáo y học: "Insulin alleviates degradation of skeletal muscle protein by inhibiting the ubiquitin-proteasome system in septic rats" pps
... These inflammatory cytokines are key factors for activity of the Ub-proteasome system [36] Thus, insulin may inhibit the activity of the Ub system by inhibiting inflammatory cytokines However, the ... sepsis currently exist In this study, we hypothesize that infusion of insulin would alleviate degradation of skeletal muscle protein by inhibiting the Ub-proteasome system in septic rats Materials ... as: Chen et al.: Insulin alleviates degradation of skeletal muscle protein by inhibiting the ubiquitin-proteasome system in septic rats Journal of Inflammation 2011 8:13 Submit your next manuscript...
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Báo cáo y học: "Targeting the ubiquitin proteasome pathway for the treatment of septic shock in patients" pps
... pathway Interestingly, many of the genes in these pathways are regulated by the proteasome [18] Therefore, combined with our results, this may also help explain why targeting one aspect of a signaling ... Subsequently, TRAF6 interacts with UBC13/UEV1A, a heterodimer that catalyzes the synthesis of polyubiquitin chains assembled through linkage of the carboxyl terminus of one ubiquitin molecule to an internal ... target for anti-NF-κB intervention than the IKK cascade for treatment of septic shock Clinically, our findings may help explain why Velcade has greater efficacy than the IKK inhibitor PS-1145 in...
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Báo cáo Y học: Antiproliferative proteins of the BTG/Tob family are degraded by the ubiquitin-proteasome system docx
... levels of BTG1 and BTG2 mRNAs increase in the early G1 phase of the cell cycle [4,5] and that BTG/Tob family proteins are involved in G1 arrest [7,11,13]; BTG/Tob family proteins accumulate at the ... degradation signal [40] Based on the results of our analyses of four members of the BTG/Tob family, we propose that the C-terminal region of this family controls its stability The C-terminal sequences ... had little effect on the degradation of BTG/Tob family proteins Thus, we conclude that inhibition of the proteasome activity results in the accumulation of BTG/Tob family proteins In addition,...
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Báo cáo sinh học: " HIV-1 Vpr activates the G2 checkpoint through manipulation of the ubiquitin proteasome system" doc
... interact with Vpr [20] Bands a and b could not be identified Figure arrest the ubiquitin proteasome system in Vpr- induced G2 Role of Role of the ubiquitin proteasome system in Vpr- induced G2 arrest ... [9,10], HIV-1 Vif [11-13], and HIV-1 Vpu [14] In the present study, we examined in detail the potential role of the UPS in the ability of HIV-1 Vpr to induce G2 arrest Results and Discussion Proteasome ... cell cycle arrest at the G2- to-M transition [3] Therefore, we set out to directly evaluate the role of the UPS in Vpr induced G2 arrest We resorted to two different methods of proteasome inhibition:...
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Báo cáo hóa học: " HIV-1 Vpr activates the G2 checkpoint through manipulation of the ubiquitin proteasome system" pptx
... interact with Vpr [20] Bands a and b could not be identified Figure arrest the ubiquitin proteasome system in Vpr- induced G2 Role of Role of the ubiquitin proteasome system in Vpr- induced G2 arrest ... [9,10], HIV-1 Vif [11-13], and HIV-1 Vpu [14] In the present study, we examined in detail the potential role of the UPS in the ability of HIV-1 Vpr to induce G2 arrest Results and Discussion Proteasome ... cell cycle arrest at the G2- to-M transition [3] Therefore, we set out to directly evaluate the role of the UPS in Vpr induced G2 arrest We resorted to two different methods of proteasome inhibition:...
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AN INVESTIGATION OF THE ROLE OF FC GAMMA RECEPTORS IN DENGUE VIRUS NEUTRALIZATION
... critical role in binding host cell receptors DII consist of the fusion loop that interacts with the endosomal membrane for fusion of the virus during entry into the cell (Rey et al, 1995) The hinge region ... expressing Fc RI and Fc RII (THP-1) and human myelogenous erythroleukemic cells (K562) expressing Fc RII, this thesis examined how different activating Fc Rs influence the amount of antibody required ... activated following infection with the h3H5 neutralizing antibody opsonized DENV in the presence of Fc RI …………… 89 3.2.2 Deriving a stable Fc RI and Fc RIIA knockdown cell line 95 3.2.3 Akt and 4EBP-1...
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Báo cáo khoa học:" A role for the JAK-STAT1 pathway in blocking replication of HSV-1 in dendritic cells and macrophages" docx
... disruption of the STAT1 gene in mice revealed a role for STAT1 in the JAK-STAT signaling pathway [40] The JAK-STAT signaling pathway is involved in mediating biologic responses induced by many cytokines ... Post Infection Figure Replication of HSV-1 in macrophages Replication of HSV-1 in macrophages Analyses of virus replication, viral gB mRNA, and viral genomic DNA in macrophages was done as in Fig ... bp; and 4) gB: forward primer, 5'AACGCGACGCACATCAAG-3', reverse primer, 5'-CTGGTACGCGATCAGAAAGC-3'; and probe, 5'-FAMCAGCCGCAGTACTACC-3' – Amplicon length = 72 bp As an internal control, a set of...
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Báo cáo khoa học: " A role for the JAK-STAT1 pathway in blocking replication of HSV-1 in dendritic cells and macrophages" pps
... disruption of the STAT1 gene in mice revealed a role for STAT1 in the JAK-STAT signaling pathway [40] The JAK-STAT signaling pathway is involved in mediating biologic responses induced by many cytokines ... Post Infection Figure Replication of HSV-1 in macrophages Replication of HSV-1 in macrophages Analyses of virus replication, viral gB mRNA, and viral genomic DNA in macrophages was done as in Fig ... bp; and 4) gB: forward primer, 5'AACGCGACGCACATCAAG-3', reverse primer, 5'-CTGGTACGCGATCAGAAAGC-3'; and probe, 5'-FAMCAGCCGCAGTACTACC-3' – Amplicon length = 72 bp As an internal control, a set of...
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Báo cáo y học: " Role of the Fas/FasL Pathway in HIV or SIV Disease" ppsx
... preserved by the treatment Another indication of the importance for indirect cell killing comes from studies of "naturally-infected" macaques i.e., sooty mangabeys infected with SIVsmm that maintain ... sparing the fraction of infected cells Such a process will tend to increase the proportion of infected cells and diminish the proportion of uninfected cells in a tissue heavily burdened by SIV or ... pathogenesis of HIV/ AIDS http://www.retrovirology.com/content/6/1/91 Significance of indirect cell killing for HIV vaccine/ therapeutics design Knowing the role for uninfected bystander cell killing in...
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The role of host cell ether lipids in influenza virus infection
... around the involvement of lipids during an influenza virus infection There is still a substantial lack in the understanding of the role of lipids during virus infections, especially influenza virus ... 1.2.2 Host proteins and their involvement in influenza virus infection .8 1.2.3 Involvement of host metabolism in influenza virus infection 11 1.2.3.1 Glycolytic flux in influenza virus infection ... implicated in the remodelling of functional ordered domains 57 4.1.3 Ether lipids, specifically ether PCs could play key roles in influenza virus infection 60 4.2 Host cell ether lipids...
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Từ khóa: benefits and risks of manipulating the hif hydroxylase pathway in ischemic heart diseasechange in the share of resources coming from households in tertiary education institutions expenditures 1995 2005 pointsa key regulator of the virus life cyclecoronaviruses gbf1 arf1 had also been shown to be important for other plus strand rna viruses such as enterovirus 240 247 248 249 and hepatitis c virus hcv 250 we tested the effect of gbf and arf1 knockdown on dengue virus infectiousers e g designers machinists etc in a product life cycle product information is deposited in the local database of each clientk nonami t nakao a harada a kurokawa t sugiyama s fujitsuka n shimomura y hutson sm harris ra takagi h 1996 the valine catabolic pathway in human liver effect of cirrhosis on enzyme activities hepatology 24 1395 1398gaosong ramsden l and corke h 1996 multipurpose uses of taro colocasia esculenta review in proceedings of the international symposium held in nanning guangxi china november 11 15 1996— investigation of low strength test results in new construction using aci 318explain the various phases in project management life cycle pdfimportance of documentation in software development life cyclethe prosperous community social capital and public life putnam 1993a comparative analysis of different types of models in software development life cyclethe green alga chlamydomonas as a tool to study the nitrate assimilation pathway in plantsepsilon neurofibromin and the ras mapk pathway in neuroblastoma differentiationthe hedgehog signaling pathway in cancer pathogenesis and therapyBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Báo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXChuong 2 nhận dạng rui roKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015