... quantify the ecotoxicity level of river water from an urban area in Japan and agricultural drains when agricultural chemicals are applied. In addition, analysis of agricultural chemicals by ... 15 6-1 66. Liu R., Kameya T., Sawai A. and Urano K. (2007). Application of a larval medaka assay to evaluate the fish safety level in Sagami River, Japan, Environ. Monit. Assess., 130, 47 5-4 82. ... - 229 - CONCLUSIONS In this study, the water quality of environmental waters based on aquatic ecotoxicity was investigated using a three-species ecotoxicity test (algae, daphnia and...