... compilation ª 2009 FEBS The effects of a-secretase ADAM10 on the proteolysis of neuregulin-1 Christian Freese1,*, Alistair N. Garratt2, Falk Fahrenholz1and Kristina Endres11 Institute of Biochemistry, ... interact.Reconstitution experiments with transfection of ADAM10 in ADAM17) ⁄ ) embryonic mouse fibro-blasts [55] suggested only a minor influence of ADAM10 on neuregulin-1 shedding, but any positiveproof ... O-gly-cosylation of NRG-1 [54], the deviation in the size of the soluble protein fragment may depend on differentglycosylation patterns in the investigated cell lines. Inmouse brain membranes, a panel of...