investigating the mechanism of escherichia coli min protein dynamics

... Factors affecting the specificity of Fpg and OGG1 FEBS Journal 275 (2008) 3747–3760 ª 2008 The Authors Journal compilation ª 2008 FEBS 3755 Ionic strength and magnesium affect the specificity of Escherichia ... independently evaluate the effects of ionic strength and Mg 2+ on the activity and specificity of OGG1, we measured the apparent values o...

Ngày tải lên: 18/02/2014, 18:20

... the Sm complex. Mol. Cell 7, 319–329. Ó FEBS 2004 The role of the C-terminal domain on Hfq (Eur. J. Biochem. 271) 1265 The C-terminal domain of Escherichia coli Hfq increases the stability of ... On the basis of these results, we propose that the C-terminal domain could protect the interface between the subunits and stabilize the hexameric...

Ngày tải lên: 19/02/2014, 12:20

... Photochemical cross-linking of Escherichia coli Fpg protein to DNA duplexes containing phenyl(trifluoromethyl)diazirine groups Maria Taranenko 1 , ... as compared to oxidized native DNA. The conditions for the photochemical cross-linking of the reactive DNA duplexes and the Fpg protein have been optimized to yield as high as 10% of the cross-linked .....

Ngày tải lên: 20/02/2014, 11:20

... the sequences of 1CEV and 1RLA and pasted into the structural alignment. The alignment was then corrected by hand. Since the sequences of the two arginases and agmatinase greately differ in the ... in the case of E. coli agmatinase, of the loop indicated by the letter a. Fig. 5. Scheme of the binuclear manganese cluster and the localization...

Ngày tải lên: 21/02/2014, 01:21

... protein consisting of two UspA domains in tandem [17]. The small members of this family are proteins of 142–144 amino-acids long; they are acidic and presumably located in the cytoplasm like UspA. Members ... amount of GroEL was determined in the same extracts using anti -GroEL Ig. It has been previously shown that at the beginning of the stationary phase the...

Ngày tải lên: 22/02/2014, 07:20

... binding properties The affinity of wild-type and mutant forms for the cofactor was measured to evaluate the impact of the mutations on the structure of the PLP binding site. Because PLP binding ... allowing the proper positioning of the residues involved in PLP binding, substrate binding and modu- lation of the cofactor’s catalytic properties. The third cluste...

Ngày tải lên: 07/03/2014, 03:20

... Comparison of pattern of resistance of E. coli isolates from chicken and laborers Table 3 Comparison of multi -resistance of E. coli- chicken isolates and E. coli- patients isolates Tropical Medicine and ... pattern of E. coli isolated from poultry industry workers and E. coli isolated from chicken (Table 2). However, despite the similarity of...

Ngày tải lên: 08/03/2014, 09:20

... further explore the contribution of these amino acids interacting with the pyrimidine ring to the catalysis of E. coli CMP kinase. Substitution of the side chain from a well-structured protein ... which the short a-heli- cal LID domain, situated in the C-terminal moiety, and the NMP -binding (NMP bind ) domain move in an induced-fit mechanism, closing upon...

Ngày tải lên: 16/03/2014, 10:20

... half of them. The present structure of monomeric E. coli DnaG- C identifies the earlier crystal structure of the same pro- tein as a domain- swapped dimer, in which helix 5 of one monomer binds to the ... dimer of E. coli DnaG- C [4]. (E) Conformer II of the crystal structure dimer of E. coli DnaG- C [4]. X C. Su et al. Solution structure of E....

Ngày tải lên: 16/03/2014, 12:20

... is whether the mechan- ism of inducer exclusion is realized in S. coelicolor. Our observation that IIA Crr could replace the inducer exclusion function of E. coli IIA Glc by inhibition of maltose ... this hypothesis. The demonstration of the IIA Crr –MalK interaction suggests that IIA Crr may regulate the function of some of the > 140 MalK homolog...

Ngày tải lên: 17/03/2014, 23:20

... surface of the protein do not appear to be major contributors to the solubility of the AMPP fragments. The AMPP protein appears to have evolved so that the metal- binding ligands are positioned optimally ... 2007 FEBS Using directed evolution to improve the solubility of the C-terminal domain of Escherichia coli aminopeptidase P I...

Ngày tải lên: 23/03/2014, 07:20

... PPIase site of SlyD. Although coher- ent with the fact that SlyD appears to interact in the proximity of the nickel ion high-affinity motif of HypB, the exact mechanism of the SlyD HypB interaction remains ... interactions between the nickel ion and the protein is the key feature of the binding process. Further evidence that SlyD interacts with...

Ngày tải lên: 30/03/2014, 01:20

... (Eur. J. Biochem. 271) Ó FEBS 2004 Effects of Escherichia coli ribosomal protein S12 mutations on cell-free protein synthesis Namthip Chumpolkulwong 1 , Chie Hori-Takemoto 1 , Takeshi Hosaka 2 , ... University of Tokyo, Bunkyo-ku, Tokyo, Japan We examined the effects of Escherichia coli ribosomal pro- tein S12 mutations on the efficiency of cell-free...

Ngày tải lên: 30/03/2014, 13:20

... (1983) The binding of tyrosine and NAD + to chorismate mutase/ prephenate dehy- drogenase from Escherichia coli K12 and the effects of these lig- ands on the activity and self association of the enzyme. ... 2003 Mapping of chorismate mutase and prephenate dehydrogenase domains in the Escherichia coli T-protein Shuqing Chen 1, *, Sarah Vin...

Ngày tải lên: 31/03/2014, 07:20

... location of Gfp-MinD in the presence of increasing levels of MinE-M and MinE 1-33 -M 145 3-9 Localization of MinE deletion mutants in the presence of MinD and co-localization of MinE deletion ... release of H-MinD and GFP-MinC-H from phospholipid vesicles 142 3-6 The N-terminus of MinE has affinity for phospholipid vesicles 143 3-7 Localization of GFP-MinD i...

Ngày tải lên: 13/11/2014, 10:02