overloading methods
Safe carrying methods
... Hazards, Health and Safety homepage Back to Hazards, Health and Safety homepage Workplace health and safety explained What does it all mean? In simple terms, workplace health and safety aims to promote ... in section 2 they will be in breach of the law and liable for a fine of $25 000. Safe carrying methods Carrying heavy objects can put a strain on your back, arms, shoulders and k...
Ngày tải lên: 21/08/2012, 17:56
Quá tải hàm constructor overloading
... Chương 4 Quá tải hàm 105 105 I/ Quá tải hàm tạo ( constructor overloading ) Có thể quá tải hàm tạo của một lớp, nhưng không quá tải hàm hủy. Hàm tạo của lớp phải phù ... Chương 4 Quá tải hàm 130 130 V/ Tìm điạ chỉ của một hàm quá tải Trong ngôn ngữ C, để biết điạ chỉ một hàm, dùng con trỏ p trỏ đến hàm đó, chẳng...
Ngày tải lên: 04/09/2012, 15:12
MOB Subject 5 –Access methods Static and dynamic access techniques
... Computer Science 1 - MOB Mobile Internet and Surrounding 1/12 Baey, Fladenmuller – Subject 5 MOB Subject 5 Access methods Static and dynamic access techniques 1. Static access techniques 1.1 Space-division ... T transmit ) = 0,87 155 9633 Assuming that the length of a slot reservation is 5% of the length of the transmission of a packet, we obtain U = 1 / (1...
Ngày tải lên: 17/09/2012, 09:13
Glycoprotein methods protocols - biotechnology 048-9-001-013.pdf
... 10 Davies and Carlstedt ice-cold dry propan-1-ol to give a 100 mM solution. DFP is unstable in water but can be stored at –20°C in propan-1-ol. After dilution, the vial as well as ... better separation between mucins Isolation of Large Gel-Forming Mucins 3 3 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana ......
Ngày tải lên: 23/09/2012, 19:25
Glycoprotein methods protocols - biotechnology 048-9-027-044.pdf
... are, however, several other alternatives, such as 3-amino- 9-ethylcarbazole (AEC), for use with horseradish peroxidase (HRP); 5-bromo-4-chloro- 3-indolyl phosphate/nitro blue tetrazolium (BCIP/NBT); ... (1984) Histochemical identification of side chain substituted O-acetylated sialic acids: the PAT- KOH-Bh-PAS and the PAPT-KOH-Bh-PAS procedures. Histochem. J. 16, 623–639. 8. Veh, R. W., Meese...
Ngày tải lên: 23/09/2012, 19:25
Glycoprotein methods protocols - biotechnology 048-9-045-055.pdf
... nonionic detergents: 0.05% (v/v) Nonidet P-40 or Tween-20. 4. Enzyme substrates: 2,2'-azino-bis(3-ethylbenzathiazaline 6-sulfonic acid) (ABTS) (1 mg/mL, A 405nm ), O-phenyldiamine (OPD) (1 mg/mL, A 492nm ), ... Histologically-based methods for detection of mucin, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 2. Davies, R., Carlstedt, I....
Ngày tải lên: 23/09/2012, 19:25
Glycoprotein methods protocols - biotechnology 048-9-057-064.pdf
... phenol in 70 mL of glycerol. 8. Poly- L -lysine-coated slides: slides are immersed in 0.01% (w/v) poly- L -lysine for 5 min and dried at room temperature. 3. Methods 3.1. Observation and Measurement of ... provided by wrapping them in a 1–cm 2 sand- GI Adherent Mucous Gel Barrier 57 57 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edit...
Ngày tải lên: 23/09/2012, 19:25
Glycoprotein methods protocols - biotechnology 048-9-065-073.pdf
... by Western-type dot-blot procedure. Spot aliquots of the homogenates or media on nitrocel- lulose paper, using the dot-blot apparatus and air-dry for 5 min. Perform all ensuing pro- cedures in ... [ 35 S]sulfate-labeled, TCA-precipitable proteins (cpm/mL) (see Note 10). g. Mature [ 35 S]sulfate-labeled, mucin band on 4% SDS-PAGE (au/mL). h. Total concentration of mucin by dot-blotting (au/mL)...
Ngày tải lên: 23/09/2012, 19:25
Glycoprotein methods protocols - biotechnology 048-9-075-085.pdf
... with “naked” cysteine-rich domains (4–7). Histological and biochemical investigations suggested that mucous-producing tis- sues and their secretions contained a complex mixture of mucin-type glycoproteins. However, ... of the gel-forming mucins in particular, there are few separation techniques available to use with these 77 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Met...
Ngày tải lên: 23/09/2012, 19:26
Glycoprotein methods protocols - biotechnology 048-9-087-096.pdf
... is rate-zonal centrifugation, which separates molecules not on hydrodynamic volume alone but on their mass-to-volume ratio as well. The basis of rate-zonal centrifugation for this pur- pose is ... Note 5). Heterogeneity and Size Distribution of Gel-Forming Mucins 87 87 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield...
Ngày tải lên: 23/09/2012, 19:26
Glycoprotein methods protocols - biotechnology 048-9-111-119.pdf
... defined in Fig. 1. Amino Acid Analysis of Mucins 113 113 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield â Humana Press Inc., ... 9-fluorenylmethyl oxycarbonyl chloride (FMOC )- based precolumn derivatization amino acid analysis that has been extensively vali- dated (1,2). Although the detailed protocols on the...
Ngày tải lên: 23/09/2012, 19:26
Glycoprotein methods protocols - biotechnology 048-9-121-128.pdf
... window with elution profiles of PTH-threonine-GalNAc-Gal (black arrow- heads) and PTH-threonine-GalNAc (gray arrowheads) from the Applied Biosystems Procise. Stan- dard amino acids are shown for ... from Edman degrada- tion: the identification of Xaa-Pro-Xaa-Xaa as a motif for Thr-O-glycosylation. Biochem. Biophys. Res. Commun. 178, 1194–1201. 6. Laursen, R. A. (1971) Solid-phase Edman degrad...
Ngày tải lên: 23/09/2012, 19:26
Glycoprotein methods protocols - biotechnology 048-9-129-141.pdf
... Amino acid t-Butyl ether S, T, Y t-Butyl ester D, E t-Butoxycarbonyl K, H, W 2,2,5,7,8-Pentamethylchroman-6-sulfonyl R Trityl C Synthetic Peptides for Antimucin Antibodies 129 129 From: Methods ... citric acid monohydrate made up to 1 L with distilled water, pH 4.0. 5. 2,2'-azino-bis[3-ethylbenz-thiazoline-6-sulfonic acid] (ABTS) substrate solution (Sigma): 0.5 mg/mL in citrate phosph...
Ngày tải lên: 23/09/2012, 19:26
Glycoprotein methods protocols - biotechnology 048-9-143-155.pdf
... sterilized. 2. 20 mM benzyl 2-acetamido-2-deoxy-α-D-galactopyranoside (benzyl-α-GalNAc) (Sigma), filter sterilized. 3. Peptide-N 4 -( acetyl-ò-glycosaminyl) asparagine amidase (N-glycosidase F, EC 3.5.1.52) (Boehringer ... of 4-nitro blue tetrazolium chloride and 1.65 mg of 5-bromo- 4- chloro-3-indolyl-phosphate), and stop solution (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). Nitro blue tetrazo...
Ngày tải lên: 23/09/2012, 19:26
overloading methods
... trỡnh trờn Windows vi Microsoftđ .NET Ging viờn : Hồ Hoàn Kiếm Overloading Methods Áp dụng cho các toán tử : Overloading Methods public class Point { public int m_x; public int m_y; public ... Point(); result.m_x = p1.m_x + p2.m_y; result.m_y = p1.m_x + p2.m_y; return result; } } Overloading Methods static void Main(string[] args) { Point objP1 = new Point(1,1); Point objP2 =...
Ngày tải lên: 19/10/2014, 00:59