Rapid amplification of 5’cDNA end (5RACE)

... aim of this study was to develop a novel method for the detection of SBV in a simple, rapid and cost-effective manner. A set of six specific primers was designed by targeting the sequence of ... of the authors read and approved the final version of the manuscript. Acknowledgments Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isot...

Ngày tải lên: 21/06/2014, 19:20

... over-representation Over-representation of GO terms was calculated using Fisher's exact test of statistical significance, implemented in the G:profiler software [46], providing as input the primary list of 672 in silico ... PAX2/5/8 binding site of cldn7 are indicated in red. (b) Binding ability of Pax2 on the promoters of brn2, metrnl, sec31l and cldn7 in the presence of...

Ngày tải lên: 14/08/2014, 21:20

Ngày tải lên: 07/08/2012, 11:48

... chemicals were of analytical grade. Preparation of solid lipid nanoparticles Weighed amounts of soyalecithin and Dynasan were dissolved in 10 ml of dichloromethane. Certain am oun ts of 5-FU was ... complex with a variety of drugs through chemical bindings [8]. The phenome- non of nanoparticles has been applied in a number of research approaches for the improvement of...

Ngày tải lên: 25/10/2012, 11:22

... Equal number of HCH cells was plated in each well of 96-well cell culture plate. Cells were treated with 5 ng/ml of TNFα in presence or absence of different concentrations of 5-Loxin ® or ... concentrations in the culture supernatants of chondrocytes treated with 5 ng/ml of human recombinant TNFα in presence or absence of different doses of either 5-Loxin or Aflapin as...

Ngày tải lên: 25/10/2012, 11:40

... when all of the following criteria were satisfied on observation of the entire esophagus: 1) disap- pearance of the tumor lesion; 2) disappearance of ul- ceration (slough); and 3) absence of cancer ... increase in the rate of severe acute toxicities, according to the increase in the dose of 5-FU. The values of the plasma concentrations of 5-FU are listed in Table 3. The...

Ngày tải lên: 26/10/2012, 09:39

... causes of rapid evolution. For example, rapid evolution might be due to a lack of functional constraint; for example, a pseudogene might rapidly accumulate mutations because of an absence of PURIFYING ... from other species. The study of the mechanisms of speciation is one of the central areas of interest in evolutionary biology. Although the role of rapid evolution o...

Ngày tải lên: 05/03/2014, 17:20

... of 742 nucleotides containing a short 5¢-untranslated sequence of 15 bp, a single open reading frame of 591 bp, and a 3¢-untranslated sequence of 136 bp with the addition of various lengths of ... mass spectrometry. The mass values of predicted peptides were calculated on the basis of the sequence of goldfish prepro- protein. On the nano ESI-TOF-MS, a molecular ion peak in th...

Ngày tải lên: 08/03/2014, 16:20

... Education Ministry of Education National AIDS Directorate Ministry of Education & Human Resource Development Ministry of Education Ministry of Education and Youth Department of Education Ministry of Education Ministry ... projection of the impact of HIV&AIDS on education supply and demand • assessment of the implications of changes in supply for teacher recruit...

Ngày tải lên: 14/03/2014, 20:20