DETERMINING MOLECULAR MECHANSIMS OF DNA NON-HOMOLOGOUS END J

DETERMINING MOLECULAR MECHANSIMS OF DNA NON-HOMOLOGOUS END JOINING PROTEINS

... Non-homologous end joining There are four specific steps in NHEJ; DNA termini recognition, bridging of the DNA ends also known as formation of the synaptic complex, DNA end processing, and finally DNA ... break IR = ionizing radiation NHEJ = non-homologous end joining HDR = homology directed repair DNA- PK = DNA dependent protein kinase DNA- PKcs = DNA de...

Ngày tải lên : 24/08/2014, 13:50

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Tài liệu Activity 7.2: Determining the Impact of Technology on a Windows DNA Design docx

... 50 Activity 7.2: Determining the Impact of Technology on a Windows DNA Design Exercise 1: Determining Technology Implications ! Determine technology ... assigned by the instructor. 2. Identify the level of impact — high, medium, or low — that each technology type would have on the given layer of a Windows DNA design. Write your answers in the grid ... a flip chart....

Ngày tải lên : 21/12/2013, 06:16

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Tài liệu Báo cáo khoa học: Human enhancer of rudimentary is a molecular partner of PDIP46/SKAR, a protein interacting with DNA polymerase d and S6K1 and regulating cell growth docx

... variants of the human POLDIP3 gene encoding proteins of 421 amino acids with a molecular mass of 46 kDa and 392 amino acids with a molecular mass of 43 kDa, which differ only by an insertion of 29 ... the extra exon characteristic of isoform 1 ⁄ a of PDIP46 ⁄ SKAR. Fig. 2. Proteins encoded by the POLDIP3 gene and the L7 insert. (A) Schematic representation of both isof...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf

... conditional ratio of DNA- PKcs in aggregates is calculated as being >10 molecules per a single end of 2.7 kbp lin- ear DNA. Very likely, the analysis of components of Aggregation-coupled DNA end- joining ... kinase activity of DNA- PK is essential for efﬁcient DNA end- joining. The massive and functional concentration of nucleoproteins on DNA in vitro may repres...

Ngày tải lên : 19/02/2014, 06:20

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Báo cáo khoa học: Molecular dynamics of the DNA-binding domain of the papillomavirus E2 transcriptional regulator uncover differential properties for DNA target accommodation pdf

... & Berendsen HJC (1977) Numerical integration of the Cartesian equations of motion of a system with constraints: molecular dynamics of n-alkanes. J Comput Phys 23, 327–341. 39 Berendsen HJC, ... rational perturbation of the DNA- binding interface showed that, in the case of HPV-16, most of the binding energy comes from a ‘direct readout’ recognition mode [22]. In fact, rec...

Ngày tải lên : 16/03/2014, 10:20

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Báo cáo khoa học: Kinetic study of the HIV)1 DNA 3¢-end processing Single-turnover property of integrase docx

... conservation relationship for DNA substrate can be written as: DNA 0 ¼ DNA S þ½IN  DNA S þ DNA P ð2Þ where [DNA] 0 represents the total concentration of DNA. Moreover, vi 0 ¼ d DNA P  dt is constant ... (n M ) Fig. 4. Dependence of the processed product formation on DNA substrate concentration. (A) Cleavage activity of IN with various concentrations of DNA substrat...

Ngày tải lên : 16/03/2014, 13:20

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Báo cáo khoa học: End-damage-speciﬁc proteins facilitate recruitment or stability of X-ray cross-complementing protein 1 at the sites of DNA single-strand break repair docx

... enzymatic processing of endo- genous DNA lesions, and as a result of aberrant DNA topoisomerase I activity (reviewed in [1,2]). Many of the SSBs arise as a consequence of loss of the DNA base and subsequent ... recognition of the DNA substrate by PNK ⁄ Pol b [26]. Using cell extracts immunodepleted of individual BER proteins and monitoring binding ⁄ cross-linking of...

Ngày tải lên : 23/03/2014, 11:20

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Báo cáo khoa học: Analysis and biological relevance of advanced glycation end-products of DNA in eukaryotic cells ppt

... in the absence of DHA) and CEdG-modified DNA (9 h, 5 m M DHA). (B) Incubation time-dependent reduction of colony forming units (cfuÆmL )1 )of CEdG-modified plasmid DNA (0.1 lgÆlL )1 DNA, 5 mM DHA ... digestion of CEdG-modified plasmid DNA Restriction enzymes recognize a palindromic DNA sequence and cut the DNA endonucleolytically within this sequence. To determine whether CEdG...

Ngày tải lên : 30/03/2014, 04:20

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Báo cáo khoa học: " Effect of fixation time and freeze-thaw cycles on the molecular analysis of viral DNA" ppt

Ngày tải lên : 07/08/2014, 17:22

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Báo cáo khoa học: "The last 10 years have witnessed considerable progress in molecular biology, trangenesis and genomics, and the assessment of wood and end-product properties" potx

Ngày tải lên : 08/08/2014, 14:21

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