DETERMINING MOLECULAR MECHANSIMS OF DNA NON-HOMOLOGOUS END JOINING PROTEINS

... Non-homologous end joining There are four specific steps in NHEJ; DNA termini recognition, bridging of the DNA ends also known as formation of the synaptic complex, DNA end processing, and finally DNA ... break IR = ionizing radiation NHEJ = non-homologous end joining HDR = homology directed repair DNA- PK = DNA dependent protein kinase DNA- PKcs = DNA de...

Ngày tải lên: 24/08/2014, 13:50

... 50 Activity 7.2: Determining the Impact of Technology on a Windows DNA Design Exercise 1: Determining Technology Implications ! Determine technology ... assigned by the instructor. 2. Identify the level of impact — high, medium, or low — that each technology type would have on the given layer of a Windows DNA design. Write your answers in the grid ... a flip chart....

Ngày tải lên: 21/12/2013, 06:16

... variants of the human POLDIP3 gene encoding proteins of 421 amino acids with a molecular mass of 46 kDa and 392 amino acids with a molecular mass of 43 kDa, which differ only by an insertion of 29 ... the extra exon characteristic of isoform 1 ⁄ a of PDIP46 ⁄ SKAR. Fig. 2. Proteins encoded by the POLDIP3 gene and the L7 insert. (A) Schematic representation of both isof...

Ngày tải lên: 19/02/2014, 05:20

... conditional ratio of DNA- PKcs in aggregates is calculated as being >10 molecules per a single end of 2.7 kbp lin- ear DNA. Very likely, the analysis of components of Aggregation-coupled DNA end- joining ... kinase activity of DNA- PK is essential for efficient DNA end- joining. The massive and functional concentration of nucleoproteins on DNA in vitro may repres...

Ngày tải lên: 19/02/2014, 06:20

... & Berendsen HJC (1977) Numerical integration of the Cartesian equations of motion of a system with constraints: molecular dynamics of n-alkanes. J Comput Phys 23, 327–341. 39 Berendsen HJC, ... rational perturbation of the DNA- binding interface showed that, in the case of HPV-16, most of the binding energy comes from a ‘direct readout’ recognition mode [22]. In fact, rec...

Ngày tải lên: 16/03/2014, 10:20

... conservation relationship for DNA substrate can be written as: DNA 0 ¼ DNA S þ½IN  DNA S þ DNA P ð2Þ where [DNA] 0 represents the total concentration of DNA. Moreover, vi 0 ¼ d DNA P  dt is constant ... (n M ) Fig. 4. Dependence of the processed product formation on DNA substrate concentration. (A) Cleavage activity of IN with various concentrations of DNA substrat...

Ngày tải lên: 16/03/2014, 13:20

... enzymatic processing of endo- genous DNA lesions, and as a result of aberrant DNA topoisomerase I activity (reviewed in [1,2]). Many of the SSBs arise as a consequence of loss of the DNA base and subsequent ... recognition of the DNA substrate by PNK ⁄ Pol b [26]. Using cell extracts immunodepleted of individual BER proteins and monitoring binding ⁄ cross-linking of...

Ngày tải lên: 23/03/2014, 11:20

... in the absence of DHA) and CEdG-modified DNA (9 h, 5 m M DHA). (B) Incubation time-dependent reduction of colony forming units (cfuÆmL )1 )of CEdG-modified plasmid DNA (0.1 lgÆlL )1 DNA, 5 mM DHA ... digestion of CEdG-modified plasmid DNA Restriction enzymes recognize a palindromic DNA sequence and cut the DNA endonucleolytically within this sequence. To determine whether CEdG...

Ngày tải lên: 30/03/2014, 04:20

Ngày tải lên: 07/08/2014, 17:22

Ngày tải lên: 08/08/2014, 14:21