REGULATION OF EXOCYTOSIS BY SYNTAXIN 4-MUNC18C COMPLEXES

... of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell 102, 849–862. 21 Hofmann TG, Moller A, Sirma H, Zentgraf H, Taya Y, Droge W, Will H & Schmitz ML (2002) Regulation of ... Ritterhoff S, Vichalkovski A, Del Sal G & Schmitz ML (2006) Autoregulatory control of the p53 response by caspase-mediated processing of HIPK2. EMBO J 25, 1883–1894. 2...

Ngày tải lên: 16/03/2014, 00:20

... 42 levels is mainly achieved by modulation of secretases, namely by the induction of a-secretase activity, by inhibition of b-secretases and ⁄ or c-secretases, or by a combination of both. This study ... of total protein was incu- bated with 1 lg of PKCa or PKCbII (both from Santa Cruz) antibody in 500 lL of lysis buffer for 90 min at 4 °C. Protein complexes were preci...

Ngày tải lên: 16/03/2014, 01:20

... destabilizes DNp63a by both proteasomal and caspase-dependent degradation pathways. The degradation of DNp63a by TNF-a was attenuated by inhibition of NF-jB, sug- gesting that activation of NF-jB may ... perinuclear transloca- tion of p65 is shown in green only after combined treatment of TNF-a and CHX. White scale bars represent 10 lm. H o. Lee et al. Regulation of DNp63a...

Ngày tải lên: 16/03/2014, 06:20

... administra- tion of anti-IL20 antibodies leads to resolution of psoria- sis [40]. Regulation of the cytokine network at the RNA level The regulation of cytokine production is the very founda- tion of the ... design of these highly discriminating siRNAs and also for the use of siRNAs in general, due to the obvious risk of off-target effects mediated by target complementar...

Ngày tải lên: 10/08/2014, 05:21

... receptor is rapidly followed by internalization of insulin-receptor complex. This internalization of recep- tor has been implicated in receptor down regulation, attenuation of insulin sensitivity and ... 0.05 nM. B. Influence of per- centage of feedback effects on dose response curve of insulin-stimulated GLUT4 translocation. The percentage feedback rep- resents the percentage...

Ngày tải lên: 13/08/2014, 22:22

... this work [10] as regulators of viral infection, indicating the high sensitivity of the assay used for the screen. Nearly a quarter of all of the kinases found to be regulators of endocytosis in the ... mediates a plethora of biological processes including nutrient uptake, regulation of growth factor recep- tors, synaptic vesicle recycling by the nervous system, and antigen...

Ngày tải lên: 14/08/2014, 16:20

... this mutation [25,30]. Also, we did not detect any difference in the regulation of AMPK complexes containing a Regulation of AMPK a2b2c3 complexes by AMP K. Lindgren et al. 2892 FEBS Journal 274 (2007) 2887–2896 ... activation of 2–4-fold of the AMPK a2b2c3 trimers expressed in bacteria or COS7 cells, relative to the activity in the absence of AMP. Previously, allos- teric...

Ngày tải lên: 30/03/2014, 08:20

... weights of 161.8, 28.2 and 13.2 kDa, respectively. The formation of the cathepsin B ⁄ cystatin C complex was seen as a shift of the elution peak of cathepsin B (Fig. 6A). The addition of 2A2 mAb ... microliters of Abz-GIVRAK(Dnp)-OH (final concentration 1 lm) and 10 lL of 2A2 mAb or NaCl ⁄ P i were added to a well of a black microtiter plate and the reaction was initiated by...

Ngày tải lên: 18/02/2014, 11:20

... with a stochiometry of 1 : 1 of dTTP bound per subunit of dCTP deaminase. Mutational analysis of amino acid residues involved in dTTP regulation of dCTP deaminase The design of the mutant enzymes ... analyse the roles of His121 and Val122 in catalysis and regulation of dCTP deaminase. Removal of the imidazole ring in H121A was anticipa- ted to relieve or reduce inhibitio...

Ngày tải lên: 18/02/2014, 16:20

... Inhibition of the ATPase activity was then explained by a slowing down of the formation of active complex in solution. On the other hand, the acceleration of the sliding velocity (and the lower number of ... properties of an additional natural component of the thin filament framework, nebulin. Nebulin spans the entire length of thin filaments and is capable of modulating the...

Ngày tải lên: 19/02/2014, 16:20