mTORC1 CONTRIBUTES TO ER STRESS INDUCED CELL DEATH

... that Bax -induced mitochondrial lipid oxidation is relevant to Bax -induced cell death. Conversely, lipid oxidation is poorly related to the massive Bax -induced superoxide and hydrogen peroxide ... decreases, probably corresponding to ethidium -induced death. All further experiments were carried out after a 45-minute incubation. Aliquots of cells (5 · 10 6 cellsÆmL )1 ) were in...

Ngày tải lên: 23/03/2014, 21:20

... anti-apoptotic factors to prevent cell death. In particular, translocation of nuclear factor kappaB (NF-jB) transcription factors to the nucleus to drive expression of antiapoptotic proteins such as cellular ... order to trigger necrotic cell death, both groups utilize cell death stimuli that we predict would disrupt the early cell- death checkpoint. He et al. [58] found...

Ngày tải lên: 28/03/2014, 23:20

... cellular model Stimuli inducing cell death Duration of ERK activation promoting cell death Characteristics of cell death Evidence implicating MEK-ERK in cell death Reference Mouse 42GPA9 Sertoli ... 12-O-tetradeca- noylphorbol-13-acetate. In vivo ⁄ cellular model Stimuli inducing cell death Duration of ERK activation promoting cell death Characteristics of cell deat...

Ngày tải lên: 29/03/2014, 08:20

... stages of apoptotic death (R2). FSC, forward scatter (proportional to cell size); SSC, side scatter (proportional to cell com- plexity or granularity). b. Cell death in neuroblastomas after 24 hours ... con- trol cells, grown in 10% FBS, was used to account for changes in cell morphology, cell number and metabolic activity due to the serum withdrawal. Cells were treated...

Ngày tải lên: 11/08/2014, 00:22

... these two distinct stress response pathways to potentiate a cell death response. Therefore, the integration of the ER stress and osmotic stress signals into a circuit of cell death occurs through ... NRP-mediated cell death response would promote tolerance to dehydration. Conclusions We have previously demonstrated that the integration of the ER stress and osmotic...

Ngày tải lên: 11/08/2014, 11:21

... nucleated -cell apoptosis such as caspases [1,4]. Mitochondria are generally considered to be key players in cell life and death. In addition to energy supply, they have also been demonstrated to be involved ... OPZ-in- duced PS externalization) rather than a typical apoptotic inducer. The previously hypothesized role of PS externalization and its role as an adhesion fac- tor in ce...

Ngày tải lên: 16/03/2014, 04:20

... to stop the reaction, chilled on ice and lyophilized. Number average molecular mass (M n )of hydrolyzed chitosan was determined using the reducing sug- ars assay of Lever [36]. The toxic chitosan ... lLÆplate )1 )on chitosan medium. Plates were incubated at 37 °C and colonies were picked up after 48–72 h. Revertant charac- terization was completed by sequencing their chitosanase genes. Chit...

Ngày tải lên: 16/03/2014, 04:20

... aged and injured cells. In addition to internal pathways that trigger apoptosis mainly in response to cytotoxic stress, apoptosis can be induced through cell surface death receptors that contain ... Surgery, Otto-von-Guericke-University, Magdeburg, Germany 2 Division of Experimental Rheumatology, Otto-von-Guericke-University, Magdeburg, Germany 3 Institute of Immunology, Otto-von-G...

Ngày tải lên: 09/08/2014, 01:23

... shown that VP2 binds to, inte- grates into and perforates the ER membrane, whereas VP3 integrates into the ER membrane, but is not suffi- cient for perforation [14]. However, we observed that both ... virus to escape from ER on its way to the nucleus [14]. We were interested in whether the minor proteins will exhibit affinity to intracellular membranes inside the host cell when p...

Ngày tải lên: 16/02/2014, 09:20

... cooperate with PI3K inhibitors to inhibit the growth of otherwise refractory colorectal cancer cell lines [48]. Synergistic interactions between MEK inhibitors and other kinase inhibitors have also been ... Treat- ment of ERK1 ⁄ 2 pathway-addicted cancer cells with a BRAF or MEK inhibitor, resulting in an accumulation of BIM, may serve to sensitize cells to other pro-apop- totic stimu...

Ngày tải lên: 16/03/2014, 00:20