... guanidine hydro-chloride (GdnHCl), urea or elevated temperatures, theydestroy the tertiary structure of proteins which results in the loss of enzymatic activity. Regarding the secondary struc-ture ... assignmentto the protein sequence was possible.RNase A activityTo determine whether the differences in the changes of the tertiary and secondary structures are reflected in the activity of RNase ... result in a rigidity of the loop and, hence, to a proteolytically lesssusceptible state of the RNase A molecule without affecting the overall structure of the protein. Interestingly, the stabilization...