Báo cáo y học: "Identification and functional characterization of cis-regulatory elements in the apicomplexan parasite Toxoplasma gond" pptx

... gene-expression only in the presence of wild-type (WT) GLYCB. The strong evolutionary conservation of motif GLYCA in E. tenella and the significant decrease in reporter activity in the double mutant ... to their role in regulating gene expression. Further experiments are needed to fully resolve these intriguing results. Genes involved in nucleotide biosynthesis and...

Ngày tải lên: 14/08/2014, 21:20

... and trypsin in the presence and a bsence of Ca 2+ . Both enzymes generated some proteolysis near the N-terminus and/ or C-terminus of the protein leaving a large central AFP fragment intact. The ... and the core CTLD. Bands corresponding to Glu-C and trypsin enzymes were identified on the basis of molecular mass. The bands smaller than 14 kDa in the trypsin d...

Ngày tải lên: 24/03/2014, 03:21

... the paucity of the latter cells in SF. In adulthood, transitional B cells generated in the bone marrow are released into the bloodstream and transported to the spleen, where they develop into long-lived ... percentage of positive cells as assessed with flow cytometry. Values in parentheses are minimum and maximum values. b P values were calculated by comparing the...

Ngày tải lên: 09/08/2014, 14:22

... This tendency also positive ly correlates with the increasing concentration of DHPC. Protein-lipid interactions and Protein-LDLR binding of ApoE-(72-166) Proteins To identify and compare the lipid binding ... separate the lipoproteins by their charge [43]. In these experiments , the interaction of VLDL and apoE proteins increased the charge of VLDL particles, result-...

Ngày tải lên: 10/08/2014, 05:21

... our findings clearly suggest that the newly recruited ED7 + AM are involved in the regulation of the ongoing inflamma- tory process. Whether the antiinflammatory effects of IL- 10 or the proinflammatory ... pri- mary antibodies were detected using a biotin-PE/streptavidin- anti-streptavidin enhancing system and labeling of AM was analyzed by flow cytometry following gati...

Ngày tải lên: 12/08/2014, 16:20

... analysis Patients were defined as having switched medications if they discontinued the medication to which they were ini- tially randomized at any time during the study and initi- ated therapy ... participated in the study design, acquisition of data, and analysis of data. BJK had oversight of the study design. All authors participated in the inter- pretation of the...

Ngày tải lên: 11/08/2014, 17:20

... tandir injuries. In their series, most of the burn victims were children and, in most of the cases, burn injuries included the head and both hands. These results were in agreement with the find ings ... our findings. The most affected body parts in our study patients were the front and back of the torso, the feet, hands, head and neck region, major joints,...

Ngày tải lên: 13/08/2014, 23:20

... conformation to the protein during binding of the substrate is shown in the top-most portion of the protein. ATP may bind to the glycine loop on the left flap portion (Fig. 8B) and AMP may bind to the AMP ... estimated by using the method of Bradford [25]. For analysis of enzyme kinetics AK assay was done at varying concentrations of ATP and AMP and the resu...

Ngày tải lên: 21/02/2014, 00:20

... (G66, R68 and G74) of Calb I–II lack the side-chain oxygens expected to provide calcium-binding ligands. The absence of calcium-binding to the EF-hand II motif of Calb has been shown in the individual ... for the full-length proteins. Keywords: calretinin; calcium; calbindin D 28k ; EF-hand; NMR secondary structure. Calretinin (CR) and calbindin D 28k (Calb) are homologo...

Ngày tải lên: 22/02/2014, 07:20

... with internal deletions and unusual isoforms composed of soluble extracellular (ligand-binding) domains [18]. As the skin shows poly- morphism in CRFR1 expression and its functional diversity may ... the humoral and behavioral responses to systemic stress [1–4]. They also play an important role in the regulation of peripheral organ functions [2,3,5,6]. In the skin th...

Ngày tải lên: 07/03/2014, 15:20