Báo cáo y học: " Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology" pptx

... R26 Research Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology Tao Liu * , David Sims † and Buzz Baum * Addresses: ... in which mnb/DYRK1A acts. Conclusions: Using parallel RNAi screens and gene expression analyses across cell types we have identifie...

Ngày tải lên: 14/08/2014, 21:20

... manifestation of both sys- temic lupus erythematosus (SLE) and the antiphospholipid syndrome (APS). Although typically mild and asympto- matic, complications of Libman-Sacks endocarditis may include ... predisposition for the mitral and aortic valves and are nowadays seen as both a car- diac manifestation of systemic lupus erythematosus (SLE) and, more recently, of the antiph...

Ngày tải lên: 10/08/2014, 10:20

... in B. subtilis, we analysed the TatAyCy system in terms of substrate specificity and complex organization. A key aim was to probe the mechanism of the TatAyCy system in the light of suggestions that ... nonspecific [26]. Characterization of separate TatAyCy and TatAy complexes formed during overexpression of the tatAyCy genes In order to study the TatAyCy translocase complexes, E...

Ngày tải lên: 16/03/2014, 04:20

... the complexity of mappings between probesets of different plat- forms, and comparability of normalizations. Fortunately, the number of various platforms for each model organism is rel- atively low and ... identification of cell cycle-regulated genes of the yeast Saccharomyces cer- evisiae by microarray hybridization. Mol Biol Cell 1998, 9:3273-3297. 7. Allison DB, Cui X,...

Ngày tải lên: 09/08/2014, 20:21

... Ontologies; OWL: Web Ontology Language; PATO: Phenotype and Trait ontology, an ontology of phenotypic qualities; PRO: Protein Ontology; WP: Worm Phenotype ontology (sometimes WBPhenotype); XP: cross-product (that ... phenotype ontology to EQ descriptions, with each such description consisting of the following elements: Q, the type of quality (characteristic) that the genotype affects;...

Ngày tải lên: 09/08/2014, 20:21

... specified and better con- trolled using the fewest number of shRNAs. Proving real in vivo efficiency in combination therapy, as well as identifying off-target and non-specific off-target effects of ... men- tioned and downloaded from this database. In order to address the impact of virus variability in the aim of the study, different type of MSAs were downloaded from the dat...

Ngày tải lên: 11/08/2014, 21:21

... sets of CNE-associated genes from three animal phyla is very unlikely to have arisen by chance, and suggests that a core set of developmental regulatory genes may be associated with CNEs across ... body plans of different animal lineages. CNEs and the evolution of animal body plans The evolution of cis-regulatory elements is an important driv- ing force in the evolution of...

Ngày tải lên: 14/08/2014, 17:22

... intrathecally once daily for 7 days, starting from 1 day before CCI surgery. Evaluation of tactile allodynia and thermal hyperalgesia The paw withdrawal latency (PWL) to radiant heat and paw ... intensity (the ‘up -and- down’ method) (in gram, g), and data were analyzed using the nonpa- rametric method of Dixon (24). Tests were performed 1 day before CCI surgery, and 1, 3,...

Ngày tải lên: 25/10/2012, 11:48

... 2 h and 20 h to determine the substrate specificity of the enzyme. The degradation of the polymer and subsequent oligosaccharide formation was analyzed by HPAEC and MALDI-TOF MS. HPAEC analysis ... Maldi-TOF MS analysis of the hydrolysis of arabinogalactans by GALA. Three different arabinogalactans were used: potato (A), onion (B), and soy (C). Spectra were taken after 15 min (1...

Ngày tải lên: 21/02/2014, 01:21

... orthovanadate and then assayed for phosphorylation activity. (E) Phosphoamino-acid analysis of PknA. MBP–bgal control (lanes 1 and 3) and MB P–PknA fusion protein (lanes 2 and 4) after Western-blot analysis ... cell division. Finally, our study in a heterologous setting has shown the involvement of PknA in cell shape regulation; it is the first report describing the functionali...

Ngày tải lên: 22/02/2014, 04:20