Báo cáo y học: " Identification of novel transcripts with differential dorso-ventral expression in Xenopus gastrula using serial analysis of gene expression" pptx

... a list of at least 86 novel transcripts with differential expression in the dorso-ventral axis. Interest-ingly, the expression of three transcripts was independent of -catenin signaling. To ... differential expression alongthe dorso-ventral axis. Interestingly, the expression of some novel transcripts was independent of -catenin.Conclusions: Our SAGE analysis provides a list of novel transcripts ... knowl-edge of the transcriptome [32]. In addition, it seems thatSAGE analysis is particularly efficient for the identification of novel transcripts. Microarray analysis of genes involved in neural induction...

Báo cáo y học: "MALDI-TOF MS Combined With Magnetic Beads for Detecting Serum Protein Biomarkers and Establishment of Boosting Decision Tree Model for Diagnosis of Colorectal Cancer"

... protein profiling of patients with chronic liver diseases. Hepa-tology. 2005; 41:40-47. 28. Cho WC, Yip TT, Ngan RK, Yip TT, Podust VN. ProteinChip array profiling for identification of disease- ... tool for integrating separation and analysis of complex mixtures of proteins. Captured proteins are then analyzed by TOF-MS, generating a spectral map depicting approximations of the molecular ... significantly dif-ferent expression of protein mass peaks (P < 0.01) was listed by the software. Subsequently, the differen-tiated expressions of protein mass peak were ana-lyzed by discriminatory...

Báo cáo y học: "PTPN22 polymorphism and anti-cyclic citrullinated peptide antibodies in combination strongly predicts future onset of rheumatoid arthritis and has a specificity of 100% for the disease" ppsx

... development of RA. The combination gave aspecificity of 100% for diagnosing RA.IntroductionA single nucleotide polymorphism in the PTPN22 gene encod-ing the lymphoid protein tyrosine phosphatase (Lyp) ... from arginineinto tryptophan. This amino acid change disrupts the binding of Lyp to an intracellular kinase, Csk, which can then no longerinactivate another kinase, Lck, that is involved in T-cell ... antibodies and RFs were determined using enzyme-linkedimmunoassays. Genotyping was performed using an ABIPRISM 7900HT instrument and HLA-SE genes were identified using PCR sequence-specific primers....

Báo cáo y học: " ZINBA integrates local covariates with DNA-seq data to identify broad and narrow regions of enrichment, even within amplified genomic region" doc

... analyzedwithin ZINBA using 250-bp windows and an additionaloffsets of 125 bp. The set of covariates and all possiblepair-wise and three-way interactions were evaluated using the BIC, with ... ChIP-seq in that it is anantibody-free method that recovers DNA fragments thatare relatively resistant to formaldehyde crosslinking toproteins. The crosslinking profile of chromatin is likelydominated ... boundary refinementIsolating exact peak boundaries within merged regionsFollowing analysis using the mixture regression model,overlapping or adjacent windows with an enrichmentprobability greater...

Báo cáo y học: "Complicated sternal dehiscence treated with the strasbourg thoracic osteosyntheses system (STRATOS) and the transposition of greater omentum: a case report" pot

... sternaldehiscence, depending on the fractures of some rightribs, occurred again after a week.After having implanted a polypropylene patch to avoidinjuries to the underlying heart, we have initially used thevacuum ... complicationafter cardiac surgery. Obesity, osteoporosis, chronicobstructive pulmonary disease (COPD), diabetes, intake of corticosteroids and off-midline sternotomy are themain risk factors [1]. Sternal ... failure.BackgroundMiddle sternotomy is the most common access to theheart and mediastinum used in the cardiac surgery. Theincidence of sternal dehiscence with or without infectionrange from 0,5 to...

Báo cáo y học: "Serum cholesterol concentration associated with aspirin esterase activity in older people: preliminary data"

... It may account for the high variability in effective anti-coagulant doses of aspirin in the population (2, 3). Increased hydrolysis of circulating aspirin, pharmacokinetically corresponding ... two distinct aspirin hydrolysis pathways act: a spontaneous pH-dependent hydrolysis and an en-zymatic hydrolysis by plasma/serum and erythrocyte esterases (6). The circulating aspirin esterase ... activities are mainly due to butyrylcholinesterase (BChE, pseudocholinesterase) and, in part, to albumin (6). Because of higher aspirin esterase activity lead-ing to faster aspirin hydrolysis, the...

Báo cáo y học: "Retinoid X receptor and peroxisome proliferator-activated receptor-gamma agonists cooperate to inhibit matrix metalloproteinase gene expression" potx

... eachinhibit proinflammatory cytokine induction of MMP-1 andMMP-13 gene expression. In this study, we address the inhib-itory effects of adding both ligands on MMP-1 and MMP-13 expression in ... MMP-13 with a polyclonal MMP-13 antibody generously provided byPeter Mitchell (Pfizer Inc, New York, NY, USA). Protein bandswere visualized by incubation with a goat anti-rabbit secondaryantibody ... with an increase in PPAR at the DR-1/AP-1 siteIf LG268 and rosiglitazone increase the affinity of RXR:PPARbinding to the DR-1/AP-1 site, thereby interfering with bindingto that site by the...

Báo cáo y học: " Detecting sequence polymorphisms associated with meiotic recombination hotspots in the human genome" pptx

... largely agree with hotspots inferred byLD-based methods [15].Next, our method looks for single-locus cis-association of the variation in hotspot strength with genetic poly-morphism in relatively ... formation.Chowdhury et al. [34] identified six genetic loci asso-ciated with recombination phenotype, including one in the RNF212 gene, and also found differences in sequence polymorphisms associated with ... correlate with the varia tion of recombination rate. Interestingly,one of these SNPs is located within CCTCCCT, one of the aforementioned motifs [12]. It is known that themouse Prdm9 gene is uniquely...

Báo cáo y học: "IL-2 production correlates with effector cell differentiation in HIV-specific CD8+ T cells" doc

... phenomena using 8-color cytokine flow cytometry, and tested the hypothesis that these two findings were causallyrelated. We analyzed cytokine profiles and memory/effector phenotypes of HIV-specific ... identically to the PBMC samples,except that all stains (including cytokines) were addedduring the "surface staining" step.Sample acquisition and analysis Flow cytometry was performed using ... cells.ResultsStability of effector/memory markers in short-term stimulationWe simultaneously analyzed cytokine production andeffector/memory markers of CMV- and HIV-responsive Tcells using the gating strategy...

Báo cáo y học: "Preoperative statin is associated with decreased operative mortality in high risk coronary artery bypass patients" pot

... of statins to operat ive risk. However the absence of statin treatment was associated with an increase in mortality in the subset of high risk patients with an esti-mated risk of mortality of ... datab ase analysis wasused to examine the role of preoperative statin use in hospital mortality, for patients undergoing isolated coronaryartery bypass grafting (CABG.)Methods: The study population ... associated with lower operative mortality in high-risk patients.IntroductionThe use of 3-hydroxy-3-methyl-glutaryl coenzyme Areductase inhibitors (statins) has been shown to reducedeath, myocardial...

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