Báo cáo y học: " Protein function annotation by homology-based inference" pps

... interactions can be inferred: by identifying domain pairs that are significantly overrepresented in inter- acting proteins [72]; by coevolutionary analysis; by identi- fying correlated mutations ... (that is, not based on annotation transfer) usually provides very limited, if any, clues. Evolutionary relationships, though complicated by the ortholog/paralog dichotomy, are by far t...

Ngày tải lên: 14/08/2014, 21:20

... sizes - a very similar behavior to that observed by Yu et al. [11] for protein production. This similarity immediately leads to a possible alternative interpretation of the results by Yu et al. ... experiment, given the advances described by Yu et al. [11] and the availability of a wide range of fluorescent protein colors [18]. This type of system, in which mRNA and protein produc...

Ngày tải lên: 14/08/2014, 16:21

... rapamycin) and of down- stream effectors such as ribosomal protein S6 kinase, thus suppressing cell growth. Transcription of the Scylla and Charybdis genes is induced by hypoxia via the hypoxia-inducible ... inhibitors discovered by these screens, the paralogous pro- teins Scylla and Charybdis. These two function in the insulin pathway downstream of PI 3-kinase and the protein kina...

Ngày tải lên: 14/08/2014, 14:21

... or definite RA (range of ages 27–71 years, with a mean age (± SEM) of 51.9 ± 16.4 years) undergoing surgical syn- ovectomy, by dissociating the minced tissue enzymati- cally with HBSS (Hank’s buffered ... Extraction Kit; Qiagen, Hilden, Germany) followed by transcription in vitro by the SP6 promoter with the Roche transcription system. The recombinant RNA was quantified by the measure...

Ngày tải lên: 09/08/2014, 01:22

... stim- Figure 1 Arthritis induction by high mobility group box chromosomal protein 1 in TNFα -/- mice and controlsArthritis induction by high mobility group box chromosomal protein 1 in TNFα -/- mice ... of cytokines and endog- enous proinflammatory molecules. High mobility group box chromosomal protein 1 (HMGB1), a nuclear DNA-binding protein, proved recently to be a potent proi...

Ngày tải lên: 09/08/2014, 10:23

... Chem Neuroanat 2005, 29:127-136. 34. Hayashi Y, Nishio M, Naito Y, Yokokura H, Nimura Y, Hidaka H, Watanabe Y: Regulation of neuronal nitric-oxide synthase by calmodulin kinases. J Biol Chem 1999, ... adrenomedullin-like immunoreactivity in the rat central nervous system by light and electron microscopy. Brain Res 2000, 853:245-268. 7. Takahashi H, Watanabe TX, Nishimura M, Nakanishi...

Ngày tải lên: 10/08/2014, 05:21

... down- regulation of PLC activity [4] and intracellular metabo- lism of IP 3 by phosphomonoesterases [5-8]. Although PLC activity is modulated by depletion of enzyme substrate [4], and decay of receptor-mediated ... removed by sequen- tial sedimentation with 3% gelatin in order to remove most of the erythrocytes. Following centrifugation (280 × g at 10°C for 10 min), residual erythrocyte...

Ngày tải lên: 11/08/2014, 08:22

... pediatric cardiovascular surgery with or without cardiopulmonary bypass. Anaesthesiology 2002, 96:1078-1085. 10. Cheung YF, Leung MP, Yuen KY: Legionella pneumonia and bacteraemia in association with protein- losing ... contributed equally to this manuscript. This work was sup- ported by the Maximilian research award 1997 and by a research grant (DL) of the German Society of Paediatric C...

Ngày tải lên: 12/08/2014, 19:22

... analysis of CDK9 dephosphorylation by PP1 and PP2A in vitro. Autophos- phorylated CDK9/cyclin T1 was subjected to dephospho- rylation by PP2A and PP1. Also we analyzed the effect of dephosphorylation ... phosphorylation of CDK9. Dephosphorylation of CDK9 by PP2A prevents formation of P-TEFb/Tat/TAR RNA complex in vitro We next analyzed whether dephosphorylation of CDK9 by PP2A or by...

Ngày tải lên: 13/08/2014, 09:21

... emergency system (HEMS) in Norway, have used anaesthesiologists as pre- hospital emergency physicians for many decades [7-9]. However, anaesthesiologists active as pre-hospital emer- gency physicians ... [16] Score Definition 0 No injury or disease 1 Injuries/diseases without any need for acute physician care 2 Injuries/diseases requiring examination and therapy by a physician but hospital...

Ngày tải lên: 25/10/2012, 09:56