Báo cáo y học: "Global analysis of alternative splicing regulation by insulin and wingless signaling in Drosophila cells" ppt

... in alternative splicing induced by activation of two distinct signaling pathways, insulin and wingless, in Drosophila cells in culture. Results: Alternative splicing changes induced by insulin ... 1 Activation of insulin and wingless signaling pathways in Drosophila S2 cells. (a) Schematic representation of the insulin and wingless signal t...

Ngày tải lên: 14/08/2014, 21:20

... (F57B9.3), ins-7 (insulin/ IGF-1-like peptide), and genes with unknown functions. Interestingly, inhibition of many novel genes by RNAi resulted in hypersensitivity to cadmium, suggesting these ... 15,15'-dioxygenase like protein, was highly cadmium-inducible and inhibition of this gene by RNAi resulted in hypersensitivity to cadmium (Table 1, Fig- ure 4). Two proteins th...

Ngày tải lên: 14/08/2014, 07:21

... prevents entry of the probe. In contrast, genes that are expressed in a very small subset of embryonic cells are more likely to be detected by in situ hybridization than by microarray analysis (data ... Nucleolus Chromatin binding Chromosome Helicase RNA binding Cytosol Nuclease activity Ubiquitin conj. enzy Protein biosynthesis Mitochondrion Methyltransferase Spindle GPCR Vi...

Ngày tải lên: 14/08/2014, 07:22

... 0.7. Transcription inhibition by actinomycin D, and sampling Actinomycin D, dissolved in DMSO, was added to a final con- centration of 15 μg/ml and samples (25 ml) were collected for half-life analysis at ... cellular target(s) of the VapC toxins is not yet clear, but in eukaryotes PIN domain proteins are ribonucleases involved in nonsense- mediated decay and RNA interfer...

Ngày tải lên: 14/08/2014, 17:22

... allo-HSCT. In conclusion, analysis of the frequency of TRBV sub- family sjTRECs further support and coincide with quanti- tative detection of total sjTRECs, and whether low frequency of BV22-BD1 and ... Ger- man y) . The quality of DNA was anal yze d in 1% agarose gels stained with ethidium bromide, and the concentra- tion was determin ed by spectrophotometric anal...

Ngày tải lên: 10/08/2014, 21:23

... 2008, 17(13):1890-1903. 91. Yamagata S, Tomita K, Sato R, Niwa A, Higashino H, Tohda Y: Interleukin- 18-deficient mice exhibit diminished chronic inflammation and airway remodelling in ovalbumin-induced asthma ... W, Bodzenta-Lukaszyk A: Plasmin system regulation in an ovalbumin-induced rat model of asthma. Int Arch Allergy Immunol 2008, 147(3):190-196. 56. Lee CC, Lin WY, Wan L, T...

Ngày tải lên: 12/08/2014, 13:22

... reported findings sim- ilar to those of the study conducted by Wang and colleagues [7], in which 46 SMC(15)s were analyzed using a combination of fluorescent in situ hybridization and microsatellite ... breakpoints for Prader-Willi, Angelman, and inv dup(15) syndromes Andrew J Makoff and Rachel H Flomen Address: Department of Psychological Medicine, King's College Lo...

Ngày tải lên: 14/08/2014, 07:21

... (ESTs), and the isoform in which the intron is retained is supported by four mRNA/ESTs. (b) Testing the splicing pathway of this exon between human and mouse. Polymerase chain reaction (PCR) analysis ... identified constraints on insertion of transposed elements within introns, and they showed that gene function and expression influence insertion and fixation of dist...

Ngày tải lên: 14/08/2014, 07:21

... product. Ingenuity pathway analysis of a network of genes expressed in EB and BC data sets associated with GATA2Figure 6 Ingenuity pathway analysis of a network of genes expressed in EB and BC ... this analysis would indicate the presence of progenitors (indicated by the number of developmental genes) of erythro- cytes, leukocytes, neurons/neuronal-muscular junc...

Ngày tải lên: 14/08/2014, 08:20

... by alternative use of coding exons during splicing, resulting in proteins with quite different C-termini (Fig. 2). The final 80 amino acids of human Mnk2 (which are very similar to the C-terminus ... pathway (see accompanying review by Proud [1]). Rapamycin thus blocks the release of 4E-BP1 from eIF4E and prevents formation of complexes contain- ing eIF4E and eIF4G. ROLES...

Ngày tải lên: 23/03/2014, 21:20