Báo cáo y học: " Evidence for common short natural trans sense-antisense pairing between transcripts from protein coding genes" docx

... hypoth- esis of common sense-antisense pairing between transcripts derived from protein coding genes is viable, it is by no means proven. For one thing, there remains a conceptual difficulty. If ... regulatory RNA. Is it likely that coding RNAs might regulate each other by sense-antisense pairing? More gener- ally, if we are blind to whether an RNA is protein coding...

Ngày tải lên: 14/08/2014, 21:20

... USA, and G. Dreyfuss, University of Pennsylvania School of Medicine, Philadelphia, PA, USA, respectively. Transfections and reporter assays for mRNA stability Transient transfections by the calcium ... l M ) as indicated. Cytoplasmic lysates were assayed for GFP-MKK6 2E protein by Western blot, and for activation of the p38 MAP kinase/MK2 pathway by in vitro kinase assays with recombi...

Ngày tải lên: 31/03/2014, 08:20

... DMARD such as hydroxychloroquine in limiting the X-ray progres- sion in early RA [6]. Benefits from early DMARD therapy extend over 5 years. Borg and colleagues [7] compared early treatment with ... frequent with combination therapy at 2 years. Subsequent analyses found that delaying DMARD therapy by only a few months decreased the ability of the monotherapy to induce remis- sion [20]. The...

Ngày tải lên: 09/08/2014, 01:23

... Roubichaud GA, Roy J, Cantin R, Tremblay MJ, Tess- ier PA: HIV-1 Transcription and virus production are both accentuated by the proinflammatory myeloid-related proteins in human CD4 + T lymphocytes. J ... 95°C step for 10 minutes (for Taq activation), followed by 40 cycles of 95°C for 15 seconds, 60°C for 1 minute, and then a final melting curve analysis with a ramp from 60°C to 95°...

Ngày tải lên: 09/08/2014, 08:22

... natural antisense transcripts, most of which appear to be non -protein- coding RNAs (ncRNAs). We have recently identified and characterized a highly conserved non -coding antisense transcript for ... bioinformatics analyses revealed a theoretical basis for many other potential interactions between natural antisense transcripts and miRNAs at the binding sites of the latter. B...

Ngày tải lên: 09/08/2014, 20:22

... Biology, Indiana University, Bloomington, IN, USA, 3 Department of Psychiatry, SUNY Upstate Medical University, Syracuse, NY, USA, 4 Laboratory of Neurophenomics, Department of Psychiatry, Indiana ... Fur- ther evidence for family-genetic risk factors in attention def- icit hyperactivity disorder. Patterns of comorbidity in probands and relatives in psychiatrically and pediatrically r...

Ngày tải lên: 11/08/2014, 17:20

... transport of RNA transcribed in the same locus of chromosome from the nucleus to their destination in the cellular cytoplasm. In the cytoplasm, RNA could be quickly bound by viral proteins before two ... cytoplasm. For example, HIV-1 encodes the virus-specific protein Rev which selectively transports the unspliced viral RNAs from the nucleus to cytoplasm [25]. Moreover, unspliced HIV-...

Ngày tải lên: 13/08/2014, 13:20

... may further elevate the risk for subsequent acute illnesses, thereby initiating a spiral of events that eventually causes death [23]. Therefore, a reasonable hypothesis is that early mortality ... manuscript. Key messages • We developed a model for predicting short- term (14 days) mortality after each episode of severe sepsis, using readily available variables. The model proved very a...

Ngày tải lên: 13/08/2014, 16:20

... secondary metabolism gene cluster of M. grisea comprises 15 genes: ACE1 and SYN2 are PKS-NRPS hybrid genes; RAP1 and RAP2 code for enoyl reductases; CYP1-CYP4 for cytochrome P450 monoxygenases; ... ORFZ for an α/β- hydrolase; OXR1 and OXR2 for oxidoreductases; MFS1 codes for a transporter in the MFS superfamily; BC2 codes for a binuclear zinc finger transcription factor; OME1 code...

Ngày tải lên: 14/08/2014, 08:20

... chosen based on the proximity that representatives from A. tumefaciens have to genes. serC U Y UUUU GUUUCCCCCYYCURAU Y GC R R G Y Y 5′ YG YY Y YY Y Terminator A AG C RY RY GC GC AC GC C CA C A 5′ RU UA GC AU GU GGG ... mix- tures by the reaction of S-adenosylhomocysteine derivatives [46,47] and excess methyl iodide [48]. Equilibrium dialysis Assays were performed by adding 100 nM...

Ngày tải lên: 14/08/2014, 14:22