Báo cáo y học: "Boolean implication networks derived from large scale, whole genome microarray datasets" docx

... CNS-specific. Genome Biology 2008, 9:R157 Open Access 2008Sahooet al.Volume 9, Issue 10, Article R157 Method Boolean implication networks derived from large scale, whole genome microarray datasets Debashis ... Affymetrix U133Plus 2.0 human microar- rays, 2,154 Affymetrix 430 2.0 mouse arrays, and 450 Affymetrix Genome 1.0 Drosophila arrays were downloaded from NCBI&apo...

Ngày tải lên: 14/08/2014, 21:20

... that is primarily recognized in polyarticular JIA patients. Finally, the epitope derived from MMP-3 elicited immune responses in both subtypes of JIA and in healthy controls. Cytokine production ... following cytokines were measured: IL-1α, IL-1β, IL-5, IL-10, IL-12, IL-18, tumor necrosis factor-α (TNF-α), and IFN-γ. Cytokine analysis by lymphocyte intracellular staining and flow cytomet...

Ngày tải lên: 09/08/2014, 08:23

... paralleled by decreased joint inflammation and destruction. Conclusion The present study shows that unique ASV derived from receptors that play key roles in angiogenesis – namely, VEGF receptor type ... human tissue types from healthy or diseased tissues and from cell lines were purchased from Clontech (Mountain View, CA, USA) and from Strategene (La Jolla, CA, USA), and were pool...

Ngày tải lên: 09/08/2014, 10:23

... human exome: a comparison of whole genome and whole transcriptome sequencing Genome Biology 2010, 11:R57 Cirulli et al. Genome Biology 2010, 11:R57 http://genomebiology.com/2010/11/5/R57 Page 6 ... overall sensitivity rose from 0.39 to 0.42 and the specificity rose from 0.47 to 0.54. If restricted to PBMC-expressed genes without paralogs, the sensitivity actually dropped sligh...

Ngày tải lên: 09/08/2014, 20:22

... patient’s largest coronary vessel by 1.5 to 2 times. Coronary artery aneurysms are typically diagnosed by coronary angiography. The prognosis of coronary artery aneurysm is not well known and ... Coronary angiography showing a large coronary artery aneurysm. Right anterior oblique view of the right coronary artery with the presence of a large aneurysm involving the distal part of the arte...

Ngày tải lên: 11/08/2014, 07:20

... were matured over two days, harvested and analyzed for cell yield and mature DC phenotype by flow cytometry, and then functionally analyzed for their ability to activate allogeneic T-cell or recall antigen ... phenotypically mature mDC from adherent monocytes using ITIP in roller bottle cultures in comparison to static flask culturesFigure 1 Generation of phenotypically mature mDC from ad...

Ngày tải lên: 11/08/2014, 10:23

... pathways by capillary electro- phoresis electrospray Ionization mass spectrometry. Analyti- cal Chemistry 2002, 74:2233-2239. 27. Ishii N, Robert M, Nakayama Y, Kanai A, Tomita M: Toward large- scale ... Biosciences, Keio University, Fujisawa, Kanagawa, 252–8520, Japan. Email: Katsuyuki Yugi - chaos@sfc.keio.ac.jp; Yoichi Nakayama* - ynakayam@sfc.keio.ac.jp; Ayako Kinoshita - ayakosan@sfc....

Ngày tải lên: 13/08/2014, 23:20

... into any non-yeast micro- array platform. Results and discussion The development of a mouse whole- genome microarray in our laboratory has been an ongoing effort, and each new design has been derived ... designing a mouse whole- genome microarray, we began by examining existing designs - the NIA Mouse 22K Microarray v1.1 (Development 60-mer Oligo) [9], which became commercially a...

Ngày tải lên: 14/08/2014, 14:21

... quantification of DNA and glycosaminogly- cans (GAG) content using the pico green assay (Invitrogen, Paisley, UK) and the dimethylmethylene blue (DMMB) assay. The beads were solubilised by incubation for ... collagenase type 1 (Gibco, Paisley, UK) for four hours at 37°C. Alginate culture It is well recognised that cells derived from IVDs change their morphology and phenotype in monolayer...

Ngày tải lên: 09/08/2014, 14:22

... characteristics. Methods Study design and study population We performed an observational study using a conveni- ence sample of primary care practices. Our study was linked to an evaluation of a quality improvement ... O ‘Malley D, Shaw E, Crabtree B: Social network analysis as an analytical tool for interaction patterns in primary care practices. Ann Fam Med 2005, 3:443-448. 12. Keating NL, A...

Ngày tải lên: 11/08/2014, 05:21