Báo cáo y học: "Gene expression response in target organ and whole blood varies as a function of target organ injury phenotype" ppsx

... aminotransferase, alkaline phosphatase, aspartate aminotransferase, lactate dehydroge- nase, and sorbitol dehydrogenase. Additional data file 1 details the clinical chemistry value for each of these ... regarding the utility and applicability of the microarray technology in biological research and in particular with respect to understanding and classifying injury processes t...

Ngày tải lên: 14/08/2014, 20:22

... 5'-ggtcatggagatgtc- cgtaa-3' and 3'-accaatagagagaccaggaagaa-5'; GTSE1: 5'- acgtgaacatggatgacccta-3' and 3'-gttcgggaaccggattattta-3'; CDC2: 5'-ggtcaagtggtagccatgaaa-3' ... 5'-ggtcaagtggtagccatgaaa-3' and 3'-ccaggagggata- gaatccaag-5'; and MKI67: 5'-ccccaaccaaaagaaagtctc-3' and 3'-gactaggagctggagggctt...

Ngày tải lên: 09/08/2014, 14:20

... 5'-GGGTTCCATGGTGAAGTCAAC 5'-6-FAM-TGTCCCGACCATTGCTGTTTCCTAGG-TAMRA IL-6 5'-CAAGACCATCCAACTCATCTTG 5'-CACAGTGAGGAATGTCCACAAAC 5'-6-FAM-TCGGCAAACCTAGTGTGCTATGCCTAAGCA-TAMRA TNF-α 5'-CCAGGTTCTCTTCAAGGGACAA ... 5'-CCAGGTTCTCTTCAAGGGACAA 5'-CTCCTGGTATGAAATGGCAAATC 5'-6-FAM-CCCGACTATGTGCTCCTCACCCACA-TAMRA GRO1 5'-TGTGTTGAAGCTTCCCTTGGA 5'-TGAGAC...

Ngày tải lên: 09/08/2014, 06:22

... pre-arthritic paws (PA) and diseased paws (AA and CA) (Table 1). In addition, RNA was isolated from normal paws of naive BALB/c SCID mice and served as a baseline non-arthritic control condition (AN). ... the activation of an anti- inflammatory cascade [51]. Thus, gene expression related to pro-inflammatory and anti-inflammatory events can be detected even before the migr...

Ngày tải lên: 09/08/2014, 06:22

... func- tion and promoting Bax and Bak activation. This in turn activates the downstream caspases 3, 6, and 7, leading to increased apoptosis. Reduced expression of SPP1 corre- lated with increased apoptosis ... shown as fold-change in mRNA transcripts. Genes indicated with a red star are in the apoptosis pathway; genes indicated with a blue star are in the transforming...

Ngày tải lên: 10/08/2014, 21:23

... marker of vascular damage in a variety of disorders, including malignancy, cardiovascular diseases and autoimmune disorders such as systemic sclerosis (SSc) and vasculitides [3-9]. In healthy subjects, ... genes related t o apoptosis, for instance, anti- apoptotic genes such as RAS p21 protein activator 1 (RASA1), protein-kinases, AMP-activated alpha1 (PRKAA1) and BCL2 int...

Ngày tải lên: 12/08/2014, 14:22

... increases above about 32, the standard error also increases, indicating a loss of precision of the replicate measurements. Data analysis A common threshold value was chosen for all genes and the baseline ... 3Summary table for each gene and each brain tissue analyzedProvided is a summary table listing the following information for each gene and each brain tissue analyzed: gene...

Ngày tải lên: 14/08/2014, 07:21

... from WS. All authors have read and approved the final manuscript. Additional data files The following additional data are available. Additional data file 1 is a table listing all probes that were ... pairwise comparison included a dye swap. This culminated in a total of 30 hybridizations within each population and 20 hybridiza- tions between populations. RNA extraction and hyb...

Ngày tải lên: 14/08/2014, 08:20

... Handel MA, Nachman MW: Asymmetry and polymor- phism of hybrid male sterility during the early stages of spe- ciation in house mice. Evolution 2008, 62:50-65. 31. Oka A, Mita A, Sakurai-Yamatani ... and interpretation, and contributed to the writing and editing of the manuscript. All authors read and approved the final manuscript. Additional data files The following additio...

Ngày tải lên: 14/08/2014, 20:22

... outside of the parental range that may have been missed when applying the stringent statistical criteria. The number of DE genes was substantially increased when applying an ANOVA FDR < 0.15 (as ... genotype. Total RNA samples were isolated using TRIzol (Invitrogen, Carlsbad, CA) and purified using the RNeasy system (QIAGEN, Valencia, CA). RNA quantity and quality were assessed us...

Ngày tải lên: 12/08/2014, 05:20